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  4. Rabbit Bone morphogenetic protein 2(BMP2) ELISA kit

Rabbit Bone morphogenetic protein 2(BMP2) ELISA kit

  • 中文名稱：
    兔子骨形成蛋白(BMP2)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL002736RB
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    This Rabbit BMP2 ELISA Kit was designed for the quantitative measurement of Rabbit BMP2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 pg/mL-100 pg/mL and the sensitivity is 0.39 pg/mL.

  • 別名：
    BMP2 ELISA Kit; BMP-2Bone morphogenetic protein 2 ELISA Kit; BMP-2 ELISA Kit
  • 縮寫(xiě)：
  • Uniprot No.：
  • 種屬：
    Oryctolagus cuniculus (Rabbit)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    1.56 pg/mL-100 pg/mL
  • 靈敏度：
    0.39 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit BMP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:2 Average % 87
    Range % 80-94
    1:4 Average % 90
    Range % 85-99
    1:8 Average % 95
    Range % 85-103
    1:10 Average % 95
    Range % 84-102
  • 回收率：
    The recovery of rabbit BMP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 93 82-100
    EDTA plasma (n=4) 97 88-105
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    100 2.136 2.177 2.157 2.043
    50 1.612 1.628 1.620 1.506
    25 1.221 1.239 1.230 1.116
    12.5 0.852 0.865 0.859 0.745
    6.25 0.530 0.516 0.523 0.409
    3.12 0.351 0.365 0.358 0.244
    1.56 0.239 0.246 0.243 0.129
    0 0.112 0.115 0.114 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes, including cardiogenesis, neurogenesis, and osteogenesis. Induces cartilage and bone formation. Initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2. Once all three components are bound together in a complex at the cell surface, BMPR2 phosphorylates and activates BMPR1A. In turn, BMPR1A propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes. Can also signal through non-canonical pathways such as ERK/MAP kinase signaling cascade that regulates osteoblast differentiation. Stimulates also the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A-ATF4 pathway by stimulating EIF2A phosphorylation which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation.
  • 基因功能參考文獻(xiàn)：
    1. The transfecting capability of a BMP-2 specific vector is examined and supports the idea that BMP-2 might diminish osseointegration and implant fixation. PMID: 21762501
    2. While BMP2 expression begins at (pregastrulation) stage 1 in the hypoblast, BMP4 expression commences--distinctly delayed compared to the mouse--diffusely at (pregastrulation) stage 2; from stage 3 onwards. PMID: 21881976
    3. Maxillary sinus floor elevation using BMP-2 gene-modified bone marrow stromal cells and TCP in rabbits PMID: 21584647
    4. Data show that BMP-2, BMP-4, and BMP-7, noggin, and chordin were colocalized in rimming osteoblasts, osteoclasts, and chondrocytes. PMID: 21099737
    5. BMP2 is not suitable to regenerate osteochondral lesions completely PMID: 19185914

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    TGF-beta family
  • 數(shù)據(jù)庫(kù)鏈接：