Rabbit Immunoglobulin E,IgE ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

This Rabbit IgE ELISA Kit was designed for the quantitative measurement of Rabbit IgE protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 7.8 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.
縮寫：

IgE
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma
檢測(cè)范圍：

7.8 pg/mL-2000 pg/mL
靈敏度：

7.8 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Immunology
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IgE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	91
	Range %	84-96
1:2000	Average %	89
	Range %	82-96
1:4000	Average %	93
	Range %	87-100
1:8000	Average %	94
	Range %	86-98

回收率：

The recovery of rabbit IgE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-96
EDTA plasma (n=4)	88	85-91

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.