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  4. Rat Apoptosis regulator BAX(BAX) ELISA kit

Rat Apoptosis regulator BAX(BAX) ELISA kit

  • 中文名稱：
    大鼠細(xì)胞凋亡調(diào)節(jié)因子BAX(BAX)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL002573RA
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
        BAX (Bcl-2-associated X protein)是一種編碼細(xì)胞凋亡的蛋白質(zhì)，是Bcl-2家族的一個(gè)成員。它通過釋放細(xì)胞質(zhì)色素C促進(jìn)凋亡。BAX是細(xì)胞凋亡通路中的關(guān)鍵分子，參與調(diào)控線粒體的透性，從而導(dǎo)致細(xì)胞凋亡。BAX在正常生理過程中也具有重要作用。它在正常細(xì)胞的有絲分裂中起到促進(jìn)凋亡的作用，使得異常細(xì)胞更容易被身體識(shí)別和清除。
        華美生物所提供的Rat Apoptosis regulator BAX(BAX) ELISA kit屬于ELISA檢測(cè)試劑盒，采用雙抗夾心法定量檢測(cè)大鼠血清、血漿、組織勻漿、細(xì)胞裂解液樣本中的BAX，其靈敏度為15.6 pg/mL，檢測(cè)范圍為62.5 pg/mL-4000 pg/mL。
     
  • 別名：
    Bax; Apoptosis regulator BAX
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測(cè)范圍：
    62.5 pg/mL-4000 pg/mL
  • 靈敏度：
    15.6 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長：
    450 nm
  • 研究領(lǐng)域：
    Cell Biology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat BAX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 89  
    Range % 85-92  
    1:2 Average % 96  
    Range % 91-105  
    1:4 Average % 90  
    Range % 87-99  
    1:8 Average % 93  
    Range % 82-98  
  • 回收率：
    The recovery of rat BAX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 88-97  
    EDTA plasma (n=4) 97 93-105  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    4000 2.091 1.995 2.043 1.844  
    2000 1.751 1.776 1.764 1.565  
    1000 1.368 1.432 1.400 1.201  
    500 0.985 0.972 0.979 0.780  
    250 0.535 0.544 0.540 0.341  
    125 0.348 0.352 0.350 0.151  
    62.5 0.244 0.237 0.241 0.042  
    0 0.201 0.197 0.199    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 最新研究進(jìn)展：
    BAX是一種參與調(diào)控細(xì)胞凋亡的蛋白質(zhì)，屬于Bcl-2家族。最近的研究表明，BAX不僅在細(xì)胞凋亡中發(fā)揮重要作用，而且在多種疾病的發(fā)生中也具有重要作用。例如，BAX在癌癥、神經(jīng)系統(tǒng)疾病、肝臟疾病等的發(fā)生中都發(fā)揮了重要作用。此外，一些研究還發(fā)現(xiàn)BAX在代謝調(diào)節(jié)、免疫系統(tǒng)調(diào)節(jié)等方面也具有重要作用。
  • 功能：
    Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
  • 亞細(xì)胞定位：
    [Isoform Alpha]: Mitochondrion outer membrane; Single-pass membrane protein. Cytoplasm.; [Isoform Beta]: Cytoplasm.; [Isoform Gamma]: Cytoplasm.
  • 蛋白家族：
    Bcl-2 family
  • 組織特異性：
    Expressed in a wide variety of tissues, with highest levels in the testis and ovary.
  • 數(shù)據(jù)庫鏈接：