Rat Brain derived neurotrophic facor,BDNF ELISA Kit
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中文名稱:大鼠腦源性神經(jīng)營養(yǎng)因子(BDNF)酶聯(lián)免疫試劑盒
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貨號:CSB-E04504r
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規(guī)格:96T/48T
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價格:¥3900/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:
The Rat Brain-derived neurotrophic factor (BDNF) ELISA Kit is a reliable and accurate tool for the quantification of BDNF in rat serum, plasma, and tissue homogenates. BDNF is a protein that plays a crucial role in the development and maintenance of the nervous system, particularly in the brain. This ELISA kit detects BDNF with high sensitivity, with a detection range of 0.312 ng/mL to 20 ng/mL and a sensitivity of 0.078 ng/mL.
The assay principle is a sandwich measurement, providing quantitative results that can be obtained in as little as 1-5 hours. The kit is optimized for use with a sample volume of 50-100ul, making it a cost-effective and efficient solution for researchers.
This ELISA kit is ideal for researchers investigating cardiovascular diseases, as BDNF has been implicated in the pathophysiology of these conditions. Additionally, our kit has been widely cited in scientific literature, with over 19 publications referencing its use.
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別名:BdnfBrain-derived neurotrophic factor ELISA kit; BDNF) [Cleaved into: BDNF precursor form ELISA kit; ProBDNF)] ELISA kit
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縮寫:
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Uniprot No.:
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種屬:Rattus norvegicus (Rat)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:0.312 ng/mL-20 ng/mL
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靈敏度:0.078 ng/mL
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反應(yīng)時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Cardiovascular
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat BDNF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 105 Range % 100-110 1:2 Average % 96 Range % 90-102 1:4 Average % 92 Range % 87-99 1:8 Average % 96 Range % 91-104 -
回收率:
The recovery of rat BDNF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 96 90-102 EDTA plasma (n=4) 98 93-104 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 20 2.256 2.299 2.278 2.164 10 2.021 2.013 2.017 1.903 5 1.657 1.674 1.666 1.552 2.5 1.243 1.267 1.255 1.141 1.25 0.751 0.764 0.758 0.644 0.625 0.502 0.529 0.516 0.402 0.312 0.312 0.321 0.317 0.203 0 0.113 0.115 0.114 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- An Analysis of the Intestinal Microbiome Combined with Metabolomics to Explore the Mechanism of How Jasmine Tea Improves Depression in CUMS-Treated Rats Y Zhang, Y Lin, Y Xiong, J Huang, Z Liu,Foods,2024
- Environmental enrichment and the combined interventions of EE and metformin enhance hippocampal neuron survival and hippocampal-dependent memory in type 2 diabetic rats under stress through the BDNF-TrkB signaling pathways TR Ismail,Biomedicine & pharmacotherapy,2024
- Curcumin attenuates brain aging by reducing apoptosis and oxidative stress M Cheriki,/,2024
- Comparison of the Effects of Botulinum Toxin Doses on Nerve Regeneration in Rats with Experimentally Induced Sciatic Nerve Injury S Hwang,Toxins,2023
- In search of stress: analysis of stress-related markers in mice after hindlimb unloading and social isolation AA Naumova,Neurological research,2023
- Neuroprotective Effect of Curcumin on Factors Involved in Neurogenesis and Angiogenesis in Aged Brain M Habibian,/,2023
- Glycyrrhiza uralensis Fisch. and its active components mitigate Semen Strychni-induced neurotoxicity through regulating high mobility group box 1 (HMGB1) translocation X Duan,Biomedicine & pharmacotherapy,2023
- Intravenous infusion of small umbilical cord mesenchymal stem cells could enhance safety and delay retinal degeneration in RCS rats Q Liang,BMC ophthalmology,2023
- Peripheral nerve fibroblasts secrete neurotrophic factors to promote axon growth of motoneurons QR He,Neural regeneration research,2023
- Effect of the Biofield Energy Treated Proprietary Test Formulation for Sleep Biomarkers in the Unpredictable Chronic Stress (UCS) Animal Model UCSUCSA Model - jndc-chemistryarticles.info,/,2023
- Environmental Enrichment and Metformin Improve Metabolic Functions, Hippocampal Neuron Survival, and Hippocampal-Dependent Memory in High-Fat/High-Sucrose Diet-Induced Type 2 Diabetic Rats TR Ismail,Biology,2023
- Gut microbiota-SCFAs-brain axis associated with the antidepressant activity of berberine in CUMS rats MN Huang,Journal of affective disorders,2023
- Inosine attenuates 3-nitropropionic acid-induced Huntington's disease-like symptoms in rats via the activation of the A2AR/BDNF/TrKB/ERK/CREB signaling pathway MES El-Shamarka,Life sciences,2022
- Di(2-ethylhexyl) adipate plasticizer triggers hepatic, brain, and cardiac injury in rats: Mitigating effect of Peganum harmala oil A Behairy,Ecotoxicology and Environmental Safety,2020
- Alterations of Brain-Derived Neurotrophic Factor and Creatinine During Ramadan Fasting: A Prospective, Controlled Clinical Trial S Zakerin,IRANIAN RED CRESCENT MEDICAL JOURNAL Monthly Publication of Iranian Hospital, Dubai,2020
- Red Beetroot Extract Abrogates Chlorpyrifos-Induced Cortical Damage in Rats G Albasher,Oxid Med Cell Longev,2020
- Antidepressant Effects of a Reformulated Traditional Tablet SA Mortazavi,IRANIAN RED CRESCENT MEDICAL JOURNAL,2020
- Sevoflurane anesthesia-mediated oxidative stress and cognitive impairment in hippocampal neurons of old rats can be ameliorated by expression of brain derived … Z Xu,Neuroscience Letters,2020
- Vitamin D3 attenuates anxiety-like behavior in long-term ovariectomized rats with unpredictable mild stress BARANENKO D,BIOCELL,2019
- Synergistic effects of combined vaccination with BCG and influenza vaccines on spatial cognition and hippocampal plasticity in rats Li Q,Brain Research Bulletin,2019
- The effect of silymarin supplementation on cognitive impairment induced by diabetes in rats Yon B,Journal of Basic and Clinical Physiology and Pharmacology,2019
- Antidepressant-like effect of lipid extract of Channa striatus in postpartum model of depression in rats Abdul Shukkoor MS.et al,Evid Based Complement Alternat Med,2016
- Comparing sprint and endurance training on anxiety, depression and its relation with brain-derived neurotrophic factor in rats TaheriChadorneshin H.et al,Behav Brain Res.,2017
- effect of Intense Exercise Training on Hydrogen Peroxide, Tumor Necrosis Factor-Alpha and the Selected Neurotrophins in Rat’s Brain Taheri Chadorneshin H.et al, The Horizon of Medical Sciences,2015
- Resistance training inhibits the elevation of skeletal muscle derived-BDNF level concomitant with improvement of muscle strength in zucker diabetic rat Hee-Jae Kim.et al,Journal of Exercise Nutrition & Biochemistry,2015
- A Comparison of Resveratrol and Vitamin C Therapy on Expression of BDNF in Stressed Rat Brain Homogenate Ashwin R Rai et al,Journal Of Pharmacy,2013
- Comparing interval and continuous exercise training regimens on neurotrophic factors in rat brain Afzalpour ME et al,Physiol Behav,2015
- Enhancement of BDNF Concentration and Restoration of the Hypothalamic-Pituitary-Adrenal Axis Accompany Reduced Depressive-Like Behaviour in Stressed Al-Rahbi B et al,ScientificWorldJournal,2014
- Light-induced retinal injury enhanced neurotrophins secretion and neurotrophic effect of mesenchymal stem cells in vitro Xu W et al,Arq Bras Oftalmol,2013
- Resveratrol improves postnatal hippocampal neurogenesis and brain derived neurotrophic factor in prenatally stressed rats Madhyastha S et al,Int J Dev Neurosci,2013
相關(guān)產(chǎn)品
靶點詳情
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功能:Important signaling molecule that activates signaling cascades downstream of NTRK2. During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.; Important signaling molecule that activates signaling cascades downstream of NTRK2. Activates signaling cascades via the heterodimeric receptor formed by NGFR and SORCS2. Signaling via NGFR and SORCS2 plays a role in synaptic plasticity and long-term depression (LTD). Binding to NGFR and SORCS2 promotes neuronal apoptosis. Promotes neuronal growth cone collapse.
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基因功能參考文獻(xiàn):
- data highlight the role of miR-206 as a post-transcriptional inhibitor of BDNF at E13 in pregnant hypothyroid rats. PMID: 30119135
- BDNF inhibits the apoptosis of cardiomyocytes induced by Ang II, and it plays the role by inhibiting CaMK2 and CaN signaling pathways. PMID: 29773102
- The above results suggest that rutin preconditioning ameliorates cerebral I/R injury in OVX rats through ER-mediated BDNF-TrkB and NGF-TrkA signaling. PMID: 29420916
- BDNF levels were much higher in both the right and left motor cortices of the mild traumatic brain injury group compared to medial prefrontal cortex, which is relatively remote from the impact site PMID: 29648487
- TRPA1-mediated spontaneous [Ca(2+)]i increase modulates the spontaneous release of peptide hormones from astrocytes. PMID: 29777700
- Results indicate the BDNF system (precursor proBDNF, BDNF pro-peptide, BDNF mRNA levels, as well as TrkB and p75(NTR) receptors) in the brain of rats selectively bred for more than 85 generations for either the high level or the lack of fear-induced aggressive is extremely different at all levels, from transcription to reception, suggesting role of BDNF system in the development of highly aggressive phenotype. PMID: 29425916
- Exercise training with concomitant nitric oxide synthase inhibition improved anxiogenic behavior and spatial cognition by enhancement of BDNF and P70S6K activity in the hippocampus and cortex. PMID: 28854337
- The environmental enrichment protected against the effects of maternal separation as indicated by the lower anxiety levels and the reversal of spatial memory deficits compared to animals housed in standard conditions. These changes were associated with increased BDNF expression in the hippocampus PMID: 29545098
- that BDNF activates mTOR to up-regulate NR2B expression, which is required for inflammatory pain-related aversion in the rostral anterior cingulate cortex of rats PMID: 29353374
- Data indicate that brain-derived neurotrophic factor confers neuroprotective effects against irradiation by modulating the NFATc4/3 pathway. PMID: 29288061
- McGill APP transgenic rats exhibit differential dysregulation in NGF and BDNF neurotrophins. At early stages, intraneuronal Abeta leads to significant reductions in BDNF mRNA expression, which correlate with learning and memory deficits. At later stages, deficits in the NGF metabolic pathway could explain the paradoxical upregulation of proNGF in the absence of changes in NGF mRNA. PMID: 28865749
- findings suggested that BDNF contributed to neuronal growth and proliferation and differentiation of Neural Sstem Cellss in vitro by stimulating PI3K/AKT/GSK3beta/beta-catenin pathways. PMID: 28524001
- Results suggest that tongue motor loss impairs hippocampus-dependent cognitive function, and decreased BDNF expression in the hippocampus may be implicated in its underlying molecular mechanism. PMID: 28767193
- Cerebral hemorrhage significantly inhibited the spatial learning and memory ability of rats; the mechanism may be related to decreased cerebral expression of BDNF and NGB PMID: 28829495
- Reduced Bdnf/trkB signaling in the hippocampus of vulnerability (Roman low-avoidance) and resistance (Roman high-avoidance) rats may contribute to their more pronounced vulnerability to Stress-induced Depression. PMID: 29075579
- BDNF-induced phrenic motor facilitation requires downstream signaling via PKCtheta; but not MEK/ERK or PI3K/Akt signaling. These data are essential to understand the sequence of the cellular cascade leading to BDNF-dependent phrenic motor plasticity. PMID: 28855298
- Results indicate that the hippocampal brain-derived neurotrophic factor/TrkB signaling pathway is activated by multiple electroconvulsive seizure treatments despite the ligand-induced down-regulation of the full-length TrkB receptor. PMID: 28890399
- These findings will contribute to the development of a novel therapeutic strategy for peripheral nerve injury, which overcomes the limitations of direct administration of exogenous BDNF by using miR-1 to regulate endogenous BDNF expression. PMID: 27381812
- Data suggest that DNA methylation of BDNF exon IX is significantly increased in hippocampal neurons in zinc deficient animals as compared to control animals; expression of BDNF mRNA is decreased. PMID: 27329329
- This study demonstrated that a long-term impact of prenatal stress on brain BDNF DNA methylation and telomere biology with relevance for behavioral and health outcomes. PMID: 28330827
- Results suggest that the DNA methyltransferase-mediated DNA methylation at the BDNF promoters IVp and IXp might be involved in the regulation of BDNF gene expression in the amygdala. Further, it could also be related to minimal traumatic brain injury-induced anxiety-like behaviors via the regulation of synaptic plasticity. PMID: 28663119
- This study demonstrated that Aberrant gene expression of brain derived neurotrophic factor (Bdnf) in the hippocampus (HPC)-prefrontal cortex (PFC) network was observed in binge eating prone rats. PMID: 28821448
- these data suggest at CpG sites of the bdnf promoter, SNL-enhanced Tet1 expression promotes DNA demethylation both by converting 5 mC to 5 hmC and inhibiting DNMT binding to regulate spinal BDNF expression, hence contributing to behavioral allodynia development. PMID: 27857218
- Treadmill running protected against IFN-alpha induced depression decreases in expression of BDNF in the hippocampus and prefrontal cortex. Aerobic exercise protects against IFN-alpha induced affective and cognitive dysfunction, which is associated with increased BDNF. PMID: 28711394
- BDNF regulates the unfolded protein response via TrkB-linked JNK inactivation in the dorsal striatum after repeated cocaine administration, thus contributing to the restoration of the endoplasmic reticulum functions. PMID: 28238708
- BDNF protects the heart against septic cardiac dysfunction by reducing oxidative stress and apoptosis via Trk-B, and it does so through activation of eNOS/NO pathway. These findings provide a new treatment strategy for sepsis-induced myocardial dysfunction. PMID: 28770018
- Study found that the BDNF propeptide promoted C6 glioma cell apoptosis and decreased in-vitro cell growth. Study also found using western blot that cleaved caspase3 and B cell lymphoma 2 (Bcl2)-associated X protein abundances increased, whereas Bcl2 abundance decreased. The data suggest that the BDNF propeptide may have an inhibitory effect on glioma through activation of the caspase3 pathway. PMID: 28640008
- BDNF participates in the regulation of the dopamine system by acting on DRD3 and DAT. PMID: 28598964
- The results showed that LIPUS enhanced the Expression of Brain-Derived Neurotrophic Factor in Astrocyte Through Activation of TrkB-Akt and Calcium-CaMK signaling pathway. PMID: 27252349
- This study demonstrated that endurance training increased the BDNF in brain. PMID: 28445707
- These studies demonstrate the long-lasting negative impact of developmental alcohol exposure on hippocampal dendritic morphology and Bdnf gene expression support the implementation of exercise and complex environments PMID: 27597545
- Studies findings reveal that brain-derived neurotrophic factor has direct GABAA receptor independent effects on hypothalamic paraventricular nucleus neurones, which are modulated by local glucose concentrations. PMID: 28258626
- the decrease in BDNF secretion and the BDNF expression might depend on the disruption of microtubule structure caused by tau hyperphosphorylation PMID: 27914953
- These data suggest that methylation change in BDNF exon is involved in the regulation of BDNF expression by Abeta or SAM, and further support the view of specific epigenetic modifications of a certain BDNF gene transcript. PMID: 29305263
- Enhanced neuroprotective efficacy of bone marrow mesenchymal stem cells co-overexpressing BDNF and VEGF in a rat model of cardiac arrest-induced global cerebral ischemia. PMID: 28492549
- Regarding BDNF, the isolation-reared rats demonstrated increased BDNF mRNA levels, H3 acetylation at the BDNF gene and BDNF protein expression in the medial prefrontal cortex PMID: 27435421
- in exposure to a stressor, sex differences in BDNF methylation may be root cause of decreased BDNF levels in females and may underlie susceptibility to pathology development. PMID: 26189832
- Study found that spinal microglia were activated and that P2X4R was primarily expressed in spinal activated microglia during nicotine withdrawal (NTW). In addition, the P2X4R and BDNF expression levels in the spinal cord were upregulated during NTW. Furthermore, inhibition of microglial activation prevented thermal hyperalgesia and increased P2X4R and BDNF expression induced by NTW. PMID: 28306606
- Brain-derived neurotrophic factor (BDNF) immunocontent was increased in the hippocampus of male rats receiving tactile stimulation (TS) from postnatal day (PND) 8-14, while glucocorticoid receptors immunocontent was decreased in both TS1-7 and TS15-21 , but not TS8-14; TS is more efficient if applied over a focused period of neonatal development (PND8-14) and can reduce emotionality and increase stress coping in adulthood PMID: 27874237
- Following IL-1beta (50mug/kg ip.) administration, a higher serum IL-1beta level was detected in females than in males. In male rats, IL-1beta decreased BDNF mRNA in all the brain structures, except for the pituitary, and VEGF mRNA in the amygdala. PMID: 27744091
- BDNF heterozygosity results in marked deficits in object and fear memory, as well as male-specific alterations in cortical and hippocampal EEG activity. These changes in EEG spectral power may indicate a differential mechanism of learning and memory deficits between male and female BDNF knock-out rats. PMID: 28576309
- exposure to an acute stress upregulates Bdnf expression in the prefrontal cortex which is selectively impaired in prenatal stress female rats, an effect that was associated with changes in the modulation of the DNA demethylase Gadd45beta. PMID: 26676568
- Study microinjected BDNF followed by AMPA (or antagonists) into the commissural nucleus tractus solitarius (cNTS), before application of NaCN into the carotid body receptors (CBR), and measured changes in glucose concentrations in arterial and venous blood. Results show that BDNF prevented the inhibitory effects that AMPA injections have in cNTS on the glycemic response induced by the local application of NaCN in CBR. PMID: 28254195
- these observations demonstrated the detailed functional interaction between BDNF and mGluR II: Activation of mGluR II positively regulates self-induced BDNF expression, and, in turn, BDNF negatively regulates the gene expression of mGluR II in a neuronal activity-independent manner, in cortical neurons, but not in astrocytes. PMID: 28119091
- We find that BDNF mediates NMDAR potentiation through activation of TrkB and phosphorylation of the GluN2B subunit by the Src-family kinase Fyn PMID: 27926876
- Ouabain decreased sensitivity to negative feedback during the initial phase of reversal learning. Expression of BDNF mRNA and protein levels was downregulated in the frontal cortex which also negatively correlated with regressive errors. Findings suggest that the ouabain model of mania may be useful in understanding the neuropathophysiology that contributes to cognitive flexibility deficits and test potential treatments. PMID: 27267245
- Neurogenesis and BDNF levels were reduced in isolated and morphine-treated isolated rats as compared to group-housed rats and morphine-treated group-housed rats, respectively. PMID: 27550421
- PSD-95 mediates the dendrite arborization-promoting effect of BDNF. PMID: 28698933
- BDNF pro-peptide regulates dendritic spines via CASP3. PMID: 27310873
- results indicate that adolescent intermittent ethanol exposure increases hippocampal histone deacetylase and decreases cyclic adenosine monophosphate response element binding protein levels that may be associated with a decrease in histone H3 acetylation in the hippocampus. These epigenetic changes potentially decrease brain-derived neurotrophic factor expression and inhibit neurogenesis in the hippocampus. PMID: 26941165
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亞細(xì)胞定位:Secreted.; [BDNF precursor form]: Secreted.
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蛋白家族:NGF-beta family
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