Rat Interleukin 18,IL-18 Elisa Kit
-
中文名稱:大鼠白介素18(IL-18)酶聯(lián)免疫試劑盒
-
貨號:CSB-E04610r
-
規(guī)格:96T/48T
-
價格:¥3200/¥2500
-
其他:
產(chǎn)品詳情
-
產(chǎn)品描述:IL18(Interleukin-18,白細(xì)胞介素18)是一種由多種免疫細(xì)胞產(chǎn)生的細(xì)胞因子,屬于IL-1家族成員。IL-18主要由巨噬細(xì)胞、樹突細(xì)胞和上皮細(xì)胞等細(xì)胞合成,并參與了免疫細(xì)胞之間的相互作用和免疫炎癥的調(diào)節(jié)。IL-18在免疫細(xì)胞的激活、增殖和分化等生物學(xué)過程中發(fā)揮著重要作用,對免疫應(yīng)答和炎癥反應(yīng)起著調(diào)節(jié)作用。此外,IL-18還與多種疾病的發(fā)生和發(fā)展相關(guān),如炎癥性腸病、自身免疫病和腫瘤等。華美生物所提供的Rat Interleukin 18,IL-18 Elisa Kit屬于ELISA檢測試劑盒,采用雙抗夾心法定量檢測大鼠血清、血漿、細(xì)胞培養(yǎng)上清樣本中的IL18,其靈敏度為0.039 ng/mL,檢測范圍為0.156 ng/mL-10 ng/mL。
-
別名:Il18 ELISA Kit; Igif ELISA Kit; Interleukin-18 ELISA Kit; IL-18 ELISA Kit; Interferon gamma-inducing factor ELISA Kit; IFN-gamma-inducing factor ELISA Kit; Interleukin-1 gamma ELISA Kit; IL-1 gamma ELISA Kit
-
縮寫:
-
Uniprot No.:
-
種屬:Rattus norvegicus (Rat)
-
樣本類型:serum, plasma, cell culture supernates
-
檢測范圍:0.156 ng/mL-10 ng/mL
-
靈敏度:0.039 ng/mL
-
反應(yīng)時間:1-5h
-
樣本體積:50-100ul
-
檢測波長:450 nm
-
研究領(lǐng)域:Immunology
-
測定原理:quantitative
-
測定方法:Sandwich
-
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 96 Range % 90-102 1:2 Average % 89 Range % 84-95 1:4 Average % 100 Range % 95-106 1:8 Average % 97 Range % 92-104 -
回收率:
The recovery of rat IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 89 93-96 EDTA plasma (n=4) 102 98-107 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 10 2.357 2.387 2.372 2.218 5 1.686 1.699 1.693 1.539 2.5 1.064 1.015 1.040 0.886 1.25 0.687 0.654 0.671 0.517 0.625 0.382 0.398 0.390 0.236 0.312 0.289 0.270 0.280 0.126 0.156 0.221 0.235 0.228 0.074 0 0.151 0.157 0.154 -
數(shù)據(jù)處理:
-
貨期:3-5 working days
引用文獻(xiàn)
- 達(dá)格列凈對野百合堿誘導(dǎo)肺動脈高壓大鼠的作用及機(jī)制研究 孫倩, 王健, 劉芬, 顏素嵐, 彭建強(qiáng), 鄒瓊超,/,/
- Bushen Huoxue formula protects against renal fibrosis and pyroptosis in chronic kidney disease by inhibiting ROS/NLRP3-mediated inflammasome activation L Liao,Renal failure,2024
- Neuropeptide Y‐Mediated Gut Microbiota Alterations Aggravate Postmenopausal Osteoporosis Z Chen,Advanced science,2023
- Network Pharmacology Analysis and Machine-Learning Models Confirmed the Ability of YiShen HuoXue Decoction to Alleviate Renal Fibrosis by Inhibiting Pyroptosis MC Feng,Drug design, development and therapy,2023
- Quercetin of huoxuehuayu tongluo decoction and azithromycin combination therapy effectively improves rat tubal factor infertility by inhibiting inflammation L Shao,Iranian journal of basic medical sciences,2024
- Conditioned medium from human dental pulp stem cells treats spinal cord injury by inhibiting microglial pyroptosis T Liu,Neural regeneration research,2024
- Upregulation of circ0000381 attenuates microglial/macrophage pyroptosis after spinal cord injury Y Zhang,Neural regeneration research,2023
- Neuritin has a neuroprotective role in the rat model of acute ischemia stroke by inhibiting neuronal apoptosis and NLRP3 inflammasome H Xu,Journal of stroke and cerebrovascular diseases,2023
- Exposure to bromoxynil octanoate herbicide induces oxidative stress, inflammation, and apoptosis in testicular tissue via modulating NF-кB pathway MMF El-Nagar,Food and chemical toxicology,2023
- Metformin regulates the effects of IR and IGF-1R methylation on mast cell activation and airway reactivity in diabetic rats with asthma through miR-152-3p/DNMT1 axis D Fu,Cell Biology and Toxicology,2023
- miR-212-3p attenuates neuroinflammation of rats with Alzheimer's disease via regulating the SP1/BACE1/NLRP3/Caspase-1 signaling pathway W Nong,Bosnian journal of basic medical sciences,2023
- Human Umbilical Cord Mesenchymal Stem Cells Inhibit Pyroptosis of Renal Tubular Epithelial Cells through miR-342-3p/Caspase1 Signaling Pathway in Diabetic Nephropathy S Zheng,Stem cells international,2023
- hASCs-derived exosomal miR-155-5p targeting TGFβR2 promotes autophagy and reduces pyroptosis to alleviate intervertebral disc degeneration D Chen,Journal of orthopaedic translation,2023
- Puerarin-V Improve Mitochondrial Respiration and Cardiac Function in a Rat Model of Diabetic Cardiomyopathy via Inhibiting Pyroptosis Pathway through P2X7 Receptors S Sun,International journal of molecular sciences,2022
- Topiramate Reprofiling for the Attenuation of Cadmium-Induced Testicular Impairment in Rats: Role of NLRP3 Inflammasome and AMPK/mTOR-Linked Autophagy HH Arab,Pharmaceuticals,2022
- Dapagliflozin, sildenafil and their combination in monocrotaline-induced pulmonary arterial hypertension Y Tang,BMC pulmonary medicine,2022
- Nrf2 Inhibits the Progression of Parkinson's Disease by Upregulating AABR07032261. 5 to Repress Pyroptosis Y Zhong,Journal of Inflammation Research,2022
- Berberine ameliorates aGVHD by gut microbiota remodelling, TLR4 signalling suppression and colonic barrier repairment for NLRP3 inflammasome inhibition Yanna Zhao,Journal of cellular and molecular medicine,2022
- Curative role of mesenchymal stromal cells in chronic pancreatitis: Modulation of MAPK and TGF-β1/SMAD factors HS Taha,International journal of immunopathology and pharmacology,2021
- Effects of oleanolic acid on inflammation and metabolism in diabetic rats H Iskender,Biotechnic & Histochemistry,2021
- Forsythiae Fructus aqueous extract attenuates cisplatin-induced kaolin consumption (pica) by inhibiting NLRP3 inflammasome activation in rats Q Meng,Bioscience, Biotechnology, and Biochemistry,2021
- Oridonin ameliorates insulin resistance partially through inhibition of inflammatory response in rats subjected to chronic unpredictable mild stress L Liang,International Immunopharmacology,2021
- Astragaloside IV Protects Against Diabetic Nephropathy by Inhibiting Pyroptosis Based on NOX4/TXNIP/NLRP3 Pathway Y Zhang,Authorea,2020
- Zhen-Wu-Tang Protects IgA Nephropathy in Rats by Regulating Exosomes to Inhibit NF-κB/NLRP3 Pathway H Li,Frontiers in Pharmacology,2020
- MicroRNA-24 attenuates diabetic vascular remodeling by suppressing the NLRP3/caspase-1/IL-1β signaling pathway Z Fan,international journal of molecular medicine,2020
- Potential anti-inflammatory and immunomodulatory effects of carvacrol against ovalbumin-induced asthma in rats El-Din Y M E,Life Sciences,2019
- Unique synergistic antiviral effects of Shufeng Jiedu Capsule and oseltamivir in influenza A viral-induced acute exacerbation of chronic obstructive pulmonary disease Guang-He Fei, et al,Biomedicine & Pharmacotherapy,2019
- Suhuang antitussive capsule inhibits NLRP3 inflammasome activation and ameliorates pulmonary dysfunction via suppression of endoplasmic reticulum stress in cough variant asthma Qin W, et al,Livestock Science,2019
- Combined ischemic postconditioning and ozone postconditioning provides synergistic protection against renal ischemia and reperfusion injury through inhibiting pyroptosis Wang L, et al,Urology,2018
- Salvianolate reduces atrial fibrillation through suppressing atrial interstitial fibrosis by inhibiting TGF-β1/Smad2/3 and TXNIP/NLRP3 inflammasome signaling pathways in post-MI rats HuiliangQiu.et al,Phytomedicine,2018
- Brusatol ameliorates 2, 4, 6-trinitrobenzenesulfonic acid-induced experimental colitis in rats: Involvement of NF-κB pathway and NLRP3 inflammasome JiangtaoZhou.et al,International Immunopharmacology,2018
- Correlation between the expression of IL Guangdi Li.et al,International Journal of Molecular Medicine,2018
- Propofol Does Not Reduce Pyroptosis of Enterocytes and Intestinal Epithelial Injury After Lipopolysaccharide Challenge Xu-Yu Zhang.et al,Digestive Diseases and Sciences,2017
- Involvement of S100A8/A9-TLR4-NLRP3 Inflammasome Pathway in Contrast-Induced Acute Kidney Injury Tan X.et al,Cell Physiol Biochem.,2017
- Dietary Fructose Activates Insulin Signaling and Inflammation in Adipose Tissue: Modulatory Role of Resveratrol Mehmet Bilgehan Pektas.et al,BioMed Research International,2016
- Protective effect of adrenomedullin on contrast induced nephropathy in rats Salih Inal et al,Nefrología (Madrid),2014
相關(guān)產(chǎn)品
靶點詳情
-
最新研究進(jìn)展:IL18是一種免疫調(diào)節(jié)因子,已知可以通過激活免疫系統(tǒng)參與多種疾病的發(fā)生和發(fā)展。最新研究表明,IL18還可以通過其他途徑參與多種生理和病理過程。例如,IL18可以作為一種重要的腫瘤抑制因子,在肝癌等多種腫瘤的治療中具有潛在應(yīng)用價值。此外,IL18還被發(fā)現(xiàn)可以通過調(diào)節(jié)神經(jīng)元的代謝和凋亡參與神經(jīng)退行性疾病的發(fā)生和發(fā)展。
-
功能:A proinflammatory cytokine primarily involved in polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses. Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells.
-
基因功能參考文獻(xiàn):
- IL18 and the related markers were closely associated with the occurrence and development of deep venous thrombosis. PMID: 29786104
- These observations suggest that IL-18 exerts direct effects upon the GnRH neuron via IL-18Ralpha and acts on GnRH neurons through an autocrine or paracrine pathway. PMID: 28373090
- These findings also indicate that IL-18 is an important regulator leading to MMP-13 expression and cell migration in astrocytes. PMID: 26558633
- These results suggest that the expression of interferon-gamma via IL-18 in the lenses of OVX rats is induced by ovariectomy PMID: 26725437
- Excessive IL-18 Relate the Aggravation of Indomethacin-Induced Intestinal Ulcerogenic Lesions in Adjuvant-Induced Arthritis Rat PMID: 26424019
- results provided evidence that delivery of IL-18 and IFN-beta by BMSCs may be an excellent and promising approach to develop an effective treatment protocol for glioma therapy. PMID: 26252165
- PMA treatment during a vulnerable period can alter brain development. IL-18 and IRAK-4 appear to be important for the development of PMA induced injury. PMID: 25918710
- Remifentanil protects the liver against I/R injury by modulating hepatic IL-18/IL-18BP balance and inhibiting IL-18 signaling PMID: 25919765
- The results in vitro showed the protective effect of IL-18 on cortical neurons. PMID: 25022959
- Using Dynamic Bayesian Network inference, we identified interleukin-18 as a central node associated with neuropathic pain in chronic sciatic constriction injury in rats. PMID: 26004155
- IL-18 can augment the hepatocyte growth ability via NF-kappaB and p38/ATF2 by targeting cyclin B1, cyclin B2, cyclin A2 and Bcl-2 in BRL-3A rat liver cells. PMID: 25752290
- IL-18 serves as a positive regulator of hepatocyte proliferation. PMID: 24412291
- IL18 was significantly increased in rat benign prostatic hyperplasia tissue. PMID: 24615654
- During learning, changes in IL-18 are restricted to the dorsal hippocampus. PMID: 23747799
- Changes in macrophage IL-18 and vascular fibrinogen deopsition following injury in aging rats contribute to local inflammation and postinjury neointima formation. PMID: 24414074
- interferon-gamma increases NF-kappaB and cytokine IL-18, but decreases IL-27 in acute pancreatitis PMID: 23725508
- IFN-gamma production via IL-18 in the retinas of 60-week-old OLETF rats is caused by hyperglycemia, and plays a role in the inflammation of the OLETF rat retinas. PMID: 23823918
- Chronic exposure of Sprague-Dawley rats to second hand smoke results in a significant increase of proinflammatory cytokine IL-18 in the bronchoalveolar lavage fluid. PMID: 23392573
- IL-18 expression increases in brain following chronic neuroinflammation elicited by systemic inflammation. PMID: 22642744
- Il-18-mediated up-regulation plays a role in maintenance of blood brain barrier function following status elepticus. PMID: 22338606
- Our findings are the first report that injury of trigeminal nerve induced IL-18 upregulation in activated microglia in the Vc, suggesting a possible role of IL-18 in orofacial neuropathic pain. PMID: 23000553
- Serum IL-18 level increased in the process of deep vein thrombosis. PMID: 22318348
- Suggest that the angiotensin II/AT1/Nox1/IL-18 pathway is a critical factor in hyperplastic vascular diseases. PMID: 22636674
- The treatment of rats with anti-IL-18 antibody at the time of burn injury prevented intestine apoptosis and normalized tight junction proteins following EtOH and burn injury. PMID: 22001439
- differential expression in abortive rats as compared to of pregnant and non-pregnant rats PMID: 21920610
- These results indicate that IL-18 secreted by microglia, which was activated by C6 glioma-derived ECM, enhanced migration of C6 glioma through NO/cGMP pathway. PMID: 21442623
- IL-18 delays neutrophil apoptosis following EtOH and burn injury by modulating the pro- and antiapoptotic proteins. PMID: 20844839
- Expression of IL-18, IL-6 and oxidative stress in rat peritoneal mesothelial cells stimulated by lipopolysaccharide PMID: 21882482
- The expression of IL-18 was increased in the lenses of OLETF rat. It is possible that activated IL-18 in the lenses of OLETF rat may be related to the lens epithelial cell apotosis and lens opacification. PMID: 21591858
- IL-18 may serve as an additional marker to monitor the severity of inflammation during pancreatitis. PMID: 21273803
- Transcripts of core components of NOD-like receptor Nlrp6 inflammasome (Nlrp6, Pycard, Caspase-1) and one of its substrates, IL-18, were increased at E20 compared with E16 in fetal intestine and not lung. PMID: 21088234
- chronic elevated IL-18 levels at a supraphsiological concentration aggravated insulin resistance, enhanced vascular inflammation and remodeling, probably by increasing the level of IRAK1 and the activity of NF-kappaB PMID: 19717152
- Role in acute lung inflammation PMID: 11739527
- Up-regulation of IL-18 and IL-12 in the ileum of neonatal rats with necrotizing enterocolitis. PMID: 12032269
- IL-18 occured in the medial habenula (MHbS), the interpenducular nucleus, and in the ependymal cells surrounding the third and the lateral ventricles. Restraint stress induced a strong elevation of IL-18 in the MHbS but not in ependymal cells. PMID: 12468024
- The frequent expression of IL-18 in thyrocytes suggests that IL-18 itself might be a secreted immunomodulator in autoimmune thyroid disease PMID: 12490070
- Translational control of IL-18 expression by its 5'-UTR limits production of IL-18, resulting in restricted expression of mRNA and protein IFN-gamma in this model of crescentic glomerulonephritis(GN). Might amplify CD8+-mediated macrophage-dependent GN. PMID: 12787406
- IL-18 induces CXCL16 expression via a MyD88, IRAK1-IRAK4-TRAF6, c-Src, PI3K, Akt, JNK, AP-1 pathway PMID: 15890643
- IL-18 may have host-protective and growth-promoting functions in the testis. PMID: 16002206
- The combined insult of EtOH and burn injury results in increased CORT levels, which in turn up-regulates intestinal IL-18 levels and thereby causes altered intestinal barrier function following a combined insult of EtOH intoxication and burn injury. PMID: 16707557
- These results identify a critical role for IL-18 in neointimal formation in a rat model of vascular injury and suggest a potential role for IL-18 neutralization in the reduction of neointimal development. PMID: 16864728
- mRNA and the protein level were attenuated by post hypoxia-ischemia hypothermia and that post hypoxia-ischemia hypothermia may decrease microglia activation in the developing brain. PMID: 17010950
- Presence of neutrophils appears to be critical for IL-18-meditaed increased lung tissue edema following a combined insult of ethanol and burn injury. PMID: 17220368
- IL-18 plays a role in enhancing the lipopolysaccharide-induced neutrophilic inflammation of the lung, but does not affect the resolution of inflammation. PMID: 17352829
- IL-18 is regulated by parathyroid hormone and is required for its bone anabolic actions PMID: 18165223
- Augmented IL-18 in serum and cardiac tissue in metabolic syndrome may contribute to the coronary perivascular fibrosis. PMID: 18504504
- Rapamycin and Sanglifehrin A, but not Cyclosporin A, block IL-18 production and this novel Rapamycin blockade effect on IL-18 may contribute to the ability of Rapamycin to inhibit chronic allograft nephropathy and restenosis. PMID: 18662782
- Our results indicate that IL-18-mediated microglia/astrocyte interactions in the spinal cord have a substantial role in the generation of tactile allodynia. PMID: 19036970
- Neutrophil chemokines CINC-1/3 may have a role in IL-18-mediated increase in neutrophil O2- production and intestinal edema following alcohol intoxication and burn injury. PMID: 19497959
顯示更多
收起更多
-
亞細(xì)胞定位:Cytoplasm. Secreted.
-
蛋白家族:IL-1 family
-
數(shù)據(jù)庫鏈接:
Most popular with customers
-
Human Transforming Growth factor β1,TGF-β1 ELISA kit
Detect Range: 23.5 pg/ml-1500 pg/ml
Sensitivity: 5.8 pg/ml
-
-
-
Mouse Tumor necrosis factor α,TNF-α ELISA Kit
Detect Range: 7.8 pg/ml-500 pg/ml
Sensitivity: 1.95 pg/ml
-
-
-
-