Rat Leukotriene B4,LT-B4 ELISA Kit
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中文名稱:大鼠白三烯B4(LTB4)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E08035r
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規(guī)格:96T/48T
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價(jià)格:¥3900/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:
This Rat LT-B4 ELISA Kit was designed for the quantitative measurement of Rat LT-B4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.
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別名:N/A
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縮寫:LT-B4
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種屬:Rattus norvegicus (Rat)
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樣本類型:serum, plasma, tissue homogenates
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檢測(cè)范圍:15.6 pg/mL-1000 pg/mL
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靈敏度:3.9 pg/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Immunology
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat LT-B4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 94 Range % 89-99 1:2 Average % 97 Range % 91-103 1:4 Average % 97 Range % 92-104 1:8 Average % 93 Range % 88-97 -
回收率:
The recovery of rat LT-B4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 92 87-96 EDTA plasma (n=4) 97 93-106 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 1000 2.442 2.541 2.492 2.320 500 1.887 1.986 1.937 1.765 250 1.282 1.263 1.273 1.101 125 0.830 0.821 0.826 0.654 62.5 0.511 0.502 0.507 0.335 31.2 0.370 0.361 0.366 0.194 15.6 0.264 0.259 0.262 0.090 0 0.171 0.172 0.172 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Cannabigerol as an anti-inflammatory agent altering the level of arachidonic acid derivatives in the colon tissue of rats subjected to a high-fat high-sucrose diet K Sztolsztener, E Harasim-Symbor,Biomedicine & Pharmacotherapy,2024
- N-acetylcysteine acts as a potent anti-inflammatory agent altering the eicosanoid profile in the development of simple steatosis and its progression to hepatitis K Sztolsztener,/,2023
- Palmatine treats urticaria by reducing inflammation and increasing autophagy T Xiao,Frontiers in immunology,2023
- α-Lipoic acid–A promising agent for attenuating inflammation and preventing steatohepatitis in rats fed a high-fat diet M Zwierz,Archives of biochemistry and biophysics,2023
- Quinoa and Chia Modulate AMPK/PPAR-? Signaling in High-Fat Diet–Induced Obesity Rat Model NH Omran,Revista Brasileira de Farmacognosia,2023
- Orally administered solasodine, a steroidal glycoalkaloid, suppresses ovalbumin-induced exaggerated Th2-immune response in rat model of bronchial asthma P Arora,Chemico-biological interactions,2022
- Yan-Hou-Qing formula attenuates ammonia-induced acute pharyngitis in rats via inhibition of NF-κB and COX-2 M Xu,BMC Complementary Medicine and Therapies,2020
- Enhanced lipoxygenase/LTD4 signaling accounts for the exaggerated hypertensive and nephrotoxic effects of cyclosporine plus indomethacin in rats Helmy MW.et al,Biomedicine & Pharmacotherapy,2018
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