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  4. Rat Prostaglandin E2,PG-E2 ELISA Kit

Rat Prostaglandin E2,PG-E2 ELISA Kit

  • 中文名稱：
    大鼠前列腺素E2(PGE2)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E07967r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
        PGE2（Prostaglandin E2）是一種重要的生理調(diào)節(jié)因子，它可以影響許多生物過程，包括炎癥反應(yīng)、免疫調(diào)節(jié)、血管擴(kuò)張和疼痛傳遞等。PGE2是一種強(qiáng)烈的炎癥介質(zhì)，其含量水平在許多疾病過程中受到調(diào)節(jié)，如炎癥性腸病、類風(fēng)濕性關(guān)節(jié)炎、心血管疾病等。
        華美生物所提供的Rat Prostaglandin E2,PG-E2 ELISA Kit屬于ELISA檢測試劑盒，采用競爭法定量檢測大鼠血清、血漿、組織勻漿、細(xì)胞裂解液樣本中的PG-E2，其靈敏度為0.25 pg/mL，檢測范圍為0.5 pg/mL-100 pg/mL。
     
  • 縮寫：
    PG-E2
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    0.5 pg/mL-100 pg/mL
  • 靈敏度：
    0.25 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat PG-E2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 96  
    Range % 91-99  
    1:2 Average % 92  
    Range % 87-96  
    1:4 Average % 93  
    Range % 89-98  
    1:8 Average % 105  
    Range % 99-109  
  • 回收率：
    The recovery of rat PG-E2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 92-99  
    EDTA plasma (n=4) 101 96-104  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    100 0.228 0.224 0.226    
    25 0.365 0.348 0.357    
    8 0.560 0.539 0.550    
    2 0.813 0.787 0.800    
    0.5 1.258 1.234 1.246    
    0 2.031 2.176 2.104    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評價(jià)

問答及客戶評論

■ 客戶評論
  • Sample type: Tissue homogenate (Brain)
    Sample species: Mouse
    Review: One model group differed from the control group, the other two did not. The operation steps of the product are still very simple, and the standards are also prepared with different concentrations.

靶點(diǎn)詳情

  • 最新研究進(jìn)展：
    PGE2是一種前列腺素，與炎癥和癌癥等多種疾病有關(guān)。近期的研究表明，PGE2可以在體內(nèi)誘導(dǎo)肝癌干細(xì)胞分化為肝癌細(xì)胞，從而促進(jìn)肝癌的發(fā)生和發(fā)展。此外，研究人員還發(fā)現(xiàn)，通過靶向PGE2的合成酶和受體可以減少腫瘤的生長和轉(zhuǎn)移，從而為腫瘤治療提供新的策略。