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中文名稱:大鼠血清素受體2A/5-羥色胺受體2A(5-HT-2A)酶聯(lián)免疫試劑盒
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貨號:CSB-E14956r
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規(guī)格:96T/48T
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價格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:
This Rat HTR2A ELISA Kit was designed for the quantitative measurement of Rat HTR2A protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.
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別名:Htr2a; Htr2; 5-hydroxytryptamine receptor 2A; 5-HT-2; 5-HT-2A; Serotonin receptor 2A
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縮寫:
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Uniprot No.:
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種屬:Rattus norvegicus (Rat)
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樣本類型:serum, plasma, tissue homogenates, cell lysates
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檢測范圍:31.25 pg/mL-2000 pg/mL
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靈敏度:7.8 pg/mL
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反應(yīng)時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Neuroscience
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat 5-HT-2A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 87 Range % 82-91 1:2 Average % 104 Range % 98-110 1:4 Average % 90 Range % 85-94 1:8 Average % 84 Range % 80-88 -
回收率:
The recovery of rat 5-HT-2A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 89 84-96 EDTA plasma (n=4) 95 90-103 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 2000 2.121 2.095 2.108 2.004 1000 1.874 1.858 1.866 1.762 500 1.546 1.533 1.540 1.436 250 1.120 1.102 1.111 1.007 125 0.707 0.718 0.713 0.609 62.5 0.498 0.516 0.507 0.403 31.25 0.295 0.280 0.288 0.184 0 0.104 0.103 0.104 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻
- Neuroprotective effect of Lannea egregia Alkaloid-rich Leaf Extracts in Streptozotocin-induced Diabetic Rats BO Ajiboye, BE Ekundayo, AW Salami, AO Osukoya,Toxicology Reports,2024
- Effect of sumatriptan on acetic acid-induced experimental colitis in rats: a possible role for the 5‐HT1B/1D receptors R Hosseini,Naunyn-Schmiedeberg's archives of pharmacology,2023
- Prokinetic effects of Citrus reticulata and Citrus aurantium extract with/without Bupleurum chinense using multistress-induced delayed gastric emptying models Y Gong,Pharmaceutical biology,2023
- Gut microbiota-SCFAs-brain axis associated with the antidepressant activity of berberine in CUMS rats MN Huang,Journal of affective disorders,2023
- GLP-1 plays a protective role in hippocampal neuronal cells by activating cAMP-CREB-BDNFsignaling pathway against CORT+ HG-induced toxicity Q Ma,researchsquare,2022
- Casticin promotes osteogenic differentiation of bone marrow stromal cells and improves osteoporosis in rats by regulating nuclear factor-κB/mitogen-activated protein kinase D Zhang,International Journal of Rheumatic Diseases,2022
- Effect of chronic unpredicted mild stress-induced depression on clopidogrel pharmacokinetics in rats X Jiang,PeerJ,2022
- Anti-aging trait of whey protein against brain damage of senile rats ME El-Beeh,Anti-aging trait of whey protein against brain damage of senile rats,2022
- Ameliorative Effects of Pueraria lobata Extract on Postmenopausal Symptoms through Promoting Estrogenic Activity and Bone Markers in Ovariectomized Rats MR Lee,Evidence-Based Complementary and Alternative Medicine,2021
- Effect of Solanum macrocarpon Linn leaf aqueous extract on the brain of an alloxan-induced rat model of diabetes MA Okesola,J Int Med Res,2020
- An orally administered magnoloside A ameliorates functional dyspepsia by modulating brain-gut peptides and gut microbiota Xue Z, et al,Life Sciences,2019
- Metabolomic signatures and microbial community profiling of depressive rat model induced by adrenocorticotrophic hormone Song J, et al,Journal of Translational Medicine,2019
- Composite carbohydrate interpenetrating polyelectrolyte nano-complexes (IPNC) as a controlled oral delivery system of citalopram HCl for pediatric use Kamel R.et al,Drug Deliv Transl Res,2018
- Immediate-type allergic and protease-mediated reactions are involved in scratching behavior induced by topical application of Dermatophagoides farinae extract in NC/Nga mice Yoshihito Yamad.et al,Exp Dermatol.,2017
- Effect of L-carnitine and Hoodia gordonii Supplementation on Metabolic Markers and Physical Performance under Short term calorie restriction in rats Jain S. et al,Indian J Clin Biochem.,2015
相關(guān)產(chǎn)品
問答及客戶評論
Please tell me is your kit Rat Serotonin Receptor 2A/5-hydroxytryptamine Receptor 2A(5-HT-2A) recommended to use with fat tissue?
Tissue Homogenates 100mg tissue was rinsed with 1X PBS, homogenized in 1 ml of 1X PBS and stored overnight at -20°C. After two freeze-thaw cycles were performed to break the cell membranes, the homogenates were centrifuged for 5 minutes at 5000 x g, 2 - 8°C.
The supernate was removed and assayed immediately. Alternatively, aliquot and store samples at -20°C or -80°C. Centrifuge the sample again after thawing before the assay.
Avoid repeated freeze-thaw cycles.
In combination with our customer's feedback before, lipase can be added or repeatedly frozen and thawed, but the detailed method needs your pre-test to confirm.Pls let me know if you have any further questions. Thank you.
靶點詳情
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功能:G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances, including mescaline, psilocybin, 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates phospholipase C and a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and promotes the release of Ca(2+) ions from intracellular stores. Affects neural activity, perception, cognition and mood. Plays a role in the regulation of behavior, including responses to anxiogenic situations and psychoactive substances. Plays a role in intestinal smooth muscle contraction, and may play a role in arterial vasoconstriction.
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基因功能參考文獻:
- Our results indicate that chlorpromazine ameliorates visceral hypersensitivity and that the 5-HT2A receptor is a potential therapeutic target for treating abdominal pain and discomfort in irritable bowel syndrome. PMID: 28750135
- Bilateral diaphragm electromyography (EMG) was performed in vagal-intact and spontaneously breathing rats before and after intravenous administration of the 5-HT2A receptor antagonist Ketanserin (1mg/kg). Intravenous ketanserin significantly attenuated ipsilateral diaphragm EMG activity in C2 spinal cord hemisection animals but had no impact on diaphragm output in uninjured animals. PMID: 28711602
- Together, these data provide evidence of a direct effect of 5-HT on adipose cells, where 5-HT suppresses lipolysis and glucose uptake, which could contribute to altered systemic lipid- and glucose metabolism. PMID: 27109474
- that 5HT2A receptors mediate 3,4-methylenedioxymethamphetamine-induced PGE2 signaling and subsequent increases in glutamate PMID: 26670377
- Study aimed at further characterizing the effects of prenatal immune activation on 5-HT2A and mGlu2/3 receptor function in male and female rat offspring PMID: 26051401
- This study showed that Serotonin-2C and -2a receptor co-expression on cells in the rat medial prefrontal cortex. PMID: 25818050
- Studied the effect of a 5-HT2A receptor antagonist on pain-related behavior, endogenous 5-hydroxytryptamine (5-HT) plasma levels, and expression of 5-HT2A receptors in dorsal root ganglia (DRGs) in a rat lumbar disc herniation model. PMID: 25584948
- Following nicotine withdrawal, 5-HT2A receptor transcript level is reduced in the ventral tegmental area. PMID: 26031442
- rats were trained in an operant conditioning task while receiving fluoxetine), tianeptine (serotonin reuptake enhancer, 10mg/kg), buspirone, risperidone (5-HT2A antagonist, 1mg/kg), ondansetron (5-HT3 antagonist, 2mg/kg) or vehicle PMID: 24949809
- 5-HT can directly activate PAFs through 5-HT2A receptor and promote fibroblasts phenotypic alterations and adventitia fibrosis depending on the signaling of the TGF-beta1/Smad3 pathway. PMID: 25185755
- Findings reveal the "therapeutic" activation of serotonin 5-HT2A in the inhibition of the compulsive drinking behaviour in HD rats. PMID: 25155310
- our findings demonstrate that low levels of serotonin, interacting with 5-HT2A receptor, are able to activate proliferative signals in the thyroid epithelial cells. PMID: 24997405
- findings do not support the hypothesis that changes in 5-HT2A R function are a vital mechanism through which uncontrollable stress contributes to stress-related pathologies such as depression; it remains to be determined whether effects of stress on 5HT2A R binding depend on nature of the stressor or on characteristics of the rat strain PMID: 24823545
- The results indicate that 5-HT1A and 5-HT2A receptors in the dorsomedial nucleus of the hypothalamus play a phasic inhibitory role upon escape expression, as previously reported in the dorsal periaqueductal gray matter PMID: 23787365
- Stress and d-amphetamine withdrawal affect prefrontal 5-HT2AR mRNA expression to a similar degree. PMID: 24287377
- The data presented here suggest that the mechanisms mediating the cannabinoid-induced upregulation of 5-HT2A receptors would be brain-region specific. PMID: 23721787
- Endogenously released 5-HT inhibits A and C fiber-evoked synaptic transmission in the rat spinal cord by the facilitation of GABA/glycine and 5-HT release via 5-HT(2A) and 5-HT(3) receptors. PMID: 23399761
- Together our data suggest that 5-HT(2A) receptors activation is involved in the regulation of distribution of cytoskeleton in the dendritic spines. PMID: 23684573
- 5-HT2A receptor antagonists can reduce the activation of hepatic stellate cells in liver cirrhotic. PMID: 23362947
- This study demonistrated taht that during their adult lives the animals with hippocampal lesions exhibit different alterations, mainly at behavioural level and in the gene expression of 5-HT(2A) receptor. PMID: 23291154
- 5-HT decreasing apoptosis through 5-HT2A receptor is involved, at least in part, in pulmonary artery remolding. PMID: 23301505
- sustained activation of CB2 receptors, which up-regulates 5-HT2A receptor signaling, enhances GRK5 expression; the phosphorylation of CB2 receptors; and the beta-arrestin 2/ERK interactions. PMID: 23592773
- Results suggest that ligands acting on HTR2A likely modulate cocaine-induced locomotion via a common mechanism to influence dopamine-dependent circuitry. PMID: 23201361
- 5-HT2A receptors seem to have a pathophysiological role in the middle cerebral artery smooth muscle. PMID: 22659115
- the increased contractile responses to 5-HT in partial bladder outlet obstruction rat bladder may be partly caused by 5-HT(2A) receptor upregulation in the detrusor smooth muscles. PMID: 23344575
- Activation of 5-HT2A receptors upregulates the function of the neuronal K-Cl cotransporter KCC2. PMID: 23248270
- Secondhand smoke exposure induces transcriptional upregulation of the 5-HT(2A), ET(B) and ET(A) receptors in rat bronchial smooth muscle cells. PMID: 22952915
- Data suggest that chronic activation of cannabinoid receptor 2 in neurons of the prefrontal cortex enhances expression of serotonin 2A receptor (5-HT2A) and dopamine D2 receptor (D2 receptor) and formation of 5-HT2A/D2 receptor complexes. PMID: 22791651
- The enhanced striatal 5HT(2A) receptors modulate the ventilatory response to hypoxia. Immediate glucose resuscitation was found to ameliorate the receptor and transporter alterations. PMID: 21683764
- 5-HT2R mediates maintaining and modulatory effect of the nociceptive response. PMID: 21186576
- This study demonstrated that a complete transection at the T10 spinal level the serotonin 2A receptor mrna was up-regulation. PMID: 22056918
- Region-specific changes in 5-HT(2A) receptor mRNA expression occur in limbic system and associated areas following chronic D-amphetamine treatment. PMID: 21420940
- Upregulation of serotonin 2A receptor underlies the development of serotonin denervation supersensitivity in spinal motoneurons following spinal injury, implicating their involvement in the pathogenesis of subsequent spasticity development. PMID: 21211552
- The 5-HT2A receptor plays a complex role in the amygdaloid complex, since it can regulate amygdaloid activity by acting on different neuronal populations. PMID: 21126512
- The mRNA and protein expressions of 5-HT2A receptor increased in excessive fatigue rats and restraint stress rats. PMID: 21600121
- A pronociceptive role is supported following 5-HT2 receptor activation, most likely through modulation of 5-HT2A receptor activity on spinal nociceptive transmission under normal conditions. PMID: 21276431
- Effects of 5-HT1 and 5-HT2 serotonin receptors on anxiety- and depression-like behavior in female rats PMID: 21598629
- Data describe the changes in 5HT(2A) receptors and its transporter in the cerebral cortex of hypoxic neonatal rats and hypoxic rats resuscitated with glucose, oxygen, and epinephrine. PMID: 20857344
- 5-HT receptors and Ca(2)+ transporting/chaperone ER proteins may participate in the activating process of hepatic stellate cells PMID: 21245988
- While normal rats can compensate for loss of 5-HT(2) receptor activity, after severe spinal cord injury rats require constitutive activity in these 5-HT(2) receptors to produce locomotion. PMID: 20861436
- Changes of exploratory activity in a novel environment induced by social isolation are associated with a decrease in mRNA expression of the Htr2a serotonin receptor genes in the midbrain and in the striatum. PMID: 20662937
- Serotonin 5-HT(2)-receptor blockade, though, does not appear to contribute to the therapeutic effect of the antipsychotic clozapine. PMID: 20811878
- Data suggest that pyridoxine in combination with insulin and A. marmelose has a role in regulation of insulin synthesis and release, through hippocampal 5-HT2A and insulin receptor function. PMID: 20868513
- Hypoxic stress increased the number of total 5HT(2A) receptors along with an up-regulation of 5HT(2A) receptor gene in cerebellum PMID: 20471502
- Serotonin 2 receptor modulation of hyperthermia, corticosterone, and hippocampal serotonin depletions following serial exposure to chronic stress and methamphetamine. PMID: 19879056
- results indicate that the upregulation of 5-HT2A receptors at least partly underlies the 5-HT denervation supersensitivity of spinal motoneurons after a complete spinal transection PMID: 20085755
- Modulation of gamma rhythms is implemented through distinct subpopulations of FSi expressing 5-HT1ARs and 5-HT2ARs. PMID: 20147548
- The effect of lysergic acid diethylamide on prepulse inhibition (PPI) is mediated by the 5-HT(2A) receptor, whereas activation of the 5-HT(2A) receptor does not appear to contribute to the effect of lisuride on PPI. PMID: 19937319
- However, activation of 5HT2 receptors diminishes the subsequent inspiratory-related excitatory neurotransmission to CVNs that is recruited during the recovery from hypoxia likely exerting an inhibitory action on inspiratory-related purinergic signaling. PMID: 19772899
- effect of thyroidectomy on the expression of mRNA in the frontal cortex PMID: 12058363
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亞細胞定位:Cell membrane; Multi-pass membrane protein. Cell projection, axon. Cytoplasmic vesicle. Membrane, caveola. Cell projection, dendrite. Cell junction, synapse, presynapse.
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蛋白家族:G-protein coupled receptor 1 family
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組織特異性:Detected in adult intestine, especially in mucosal epithelium, longitudinal and circular layers of muscularis externa and myenteric plexuses. Highly expressed in Paneth cells, and detected at lower levels in enterocytes (at protein level). Detected in bra
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