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  4. Rat Thyroid Stimulating Hormone,TSH ELISA Kit

Rat Thyroid Stimulating Hormone,TSH ELISA Kit

  • 中文名稱：
    大鼠促甲狀腺素(TSH)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E05115r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
        TSH (促甲狀腺激素，thyroid-stimulating hormone) 是垂體腺細胞產(chǎn)生的一種促進甲狀腺合成和釋放甲狀腺素的激素。在人體內(nèi)，TSH的含量是由甲狀腺激素水平負(fù)反饋控制的。當(dāng)甲狀腺激素水平下降時，下丘腦和垂體腺釋放TRH和TSH來促進甲狀腺合成和釋放甲狀腺素。
        華美生物所提供的Rat Thyroid Stimulating Hormone,TSH ELISA Kit屬于ELISA檢測試劑盒，采用雙抗夾心法定量檢測大鼠血清、血漿、組織勻漿、細胞裂解液樣本中的TSH，其靈敏度為0.3 μIU/mL，檢測范圍為0.6 μIU/mL-24 μIU/mL。
     
  • 別名：
    TshbThyrotropin subunit beta ELISA kit; Thyroid-stimulating hormone subunit beta ELISA kit; TSH-B ELISA kit; TSH-beta ELISA kit; Thyrotropin beta chain ELISA kit
  • 縮寫：
    TSH
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    0.6 μIU/mL-24 μIU/mL
  • 靈敏度：
    0.3 μIU/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat TSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 93  
    Range % 87-98  
    1:2 Average % 87  
    Range % 84-90  
    1:4 Average % 91  
    Range % 85-96  
    1:8 Average % 95  
    Range % 90-99  
  • 回收率：
    The recovery of rat TSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 91-100  
    EDTA plasma (n=4) 93 88-99  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μIU/ml OD1 OD2 Average    
    24 2.756 2.781 2.769    
    12 1.997 1.988 1.993    
    6 1.142 1.124 1.133    
    3 0.634 0.613 0.624    
    1.2 0.244 0.253 0.249    
    0.6 0.115 0.117 0.116    
  • 本試劑盒所含材料：
      • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-rat TSH antibody.
      • Standard 7 x 0.5 ml--- Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
      • HRP-conjugated TSH antibody 1 x 6 ml --- Bind to the TSH in the sample or in the standard and react with the TMB substrate to make the solution chromogenic.
      • Wash Buffer (20 x concentrate) 1 x 15 ml --- Wash away unbound or free substances.
      • Substrate A1 x 7 ml --- Mix with Substrate B, and the mixture reacts with HRP to make the solution appear blue.
      • Substrate B 1 x 7 ml --- Mix with Substrate A, and the mixture reacts with HRP to make the solution appear blue.
      • Stop Solution 1 x 7 ml --- Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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  • 本試劑盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻

產(chǎn)品評價

平均分:
5.0分 - 2 個評價

樣品類型：血清

樣品信息：大鼠

稀釋比：其他 1：10

產(chǎn)品評價： 我用CSB-E05115r檢測自身免疫性甲狀腺炎大鼠，稀釋比例為1：10。甲功的elisa盒子操作步驟一樣，同一批血清可以同時做，大大縮短實驗時間，非常方便，結(jié)果準(zhǔn)確。

By 丁老師

樣品類型：血清

樣品信息：大鼠

稀釋比：沒有稀釋

產(chǎn)品評價： 我用CSB-E05115r檢測大鼠血清樣本，其OD值為0.048-0.069，產(chǎn)品使用過程簡便，說明書清晰，售后技術(shù)回答問題及時，實驗結(jié)果穩(wěn)定可用，可選擇指標(biāo)齊全。

By 陳老師

問答及客戶評論

■ 常見問題解答
Q:

Please let us know delivery time and the sulfuric acid content of [stop solution] .

A:
Thanks for your inquiry. The sulfuric acid content of [stop solution] is 4N. Delivery time is usually around 3-5 business days.

靶點詳情

  • 最新研究進展：
    TSH是一種由垂體前葉分泌的激素，它通過刺激甲狀腺合成和釋放甲狀腺激素來調(diào)節(jié)代謝。最新的研究表明，TSH不僅影響代謝，還可能在其他生理和病理過程中發(fā)揮重要作用。例如，TSH受體在某些腫瘤中表達增加，TSH可能通過調(diào)節(jié)腫瘤細胞增殖和生存來影響這些腫瘤的發(fā)展。此外，還有研究表明TSH可能與心血管疾病、精神疾病和骨代謝等方面有關(guān)。
  • 功能：
    Indispensable for the control of thyroid structure and metabolism.
  • 基因功能參考文獻：
    1. These data add new insight to an important role of T3 in rapidly regulating TSH beta gene expression at posttranscriptional level. PMID: 21078364
    2. TSH action stimulates the phosphorylation of hsp-90 in FRTL-5 thyroid cells PMID: 16910874
    3. Results clearly show that there are diurnal changes in TSH expression and accumulation in rat PT-TSH cells and suggest that these fluctuations are regulated by melatonin. PMID: 17927662
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Glycoprotein hormones subunit beta family
  • 數(shù)據(jù)庫鏈接：

    KEGG: rno:25653

    STRING: 10116.ENSRNOP00000022575

    UniGene: Rn.9966