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  4. Rat Tryptophan Hydroxylase (TPH) ELISA Kit

Rat Tryptophan Hydroxylase (TPH) ELISA Kit

  • 中文名稱：
    大鼠色氨酸羥化酶(TPH)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E13984r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    The Rat Tryptophan Hydroxylase (TPH) ELISA Kit is a powerful tool for quantitative analysis of the TPH protein in rat samples.

    TPH is an important enzyme in the synthesis of serotonin, a neurotransmitter involved in various physiological and pathophysiological processes. This ELISA kit is specifically designed for the measurement of TPH protein levels in a variety of sample types, including serum, plasma, tissue homogenates, and cell lysates from Rattus norvegicus (Rat) species.

    With a detection range of 37.5 pg/mL to 2400 pg/mL and a sensitivity of 9.4 pg/mL, this TPH ELISA kit can accurately detect even low levels of TPH protein. The assay principle is based on a sandwich immunoassay, which involves the use of two specific antibodies that bind to different epitopes of the target protein. This approach ensures high specificity and sensitivity in the measurement of TPH levels. The assay can be completed in just 1-5 hours, with a sample volume of 50-100ul, making it a fast and convenient option for research.

    This ELISA kit provides a reliable and accurate method for the quantitative analysis of TPH protein levels in rat samples, making it an essential tool for any researcher studying the role of serotonin in various physiological and pathophysiological processes.

  • 別名：
    Tph1 ELISA Kit; Tph ELISA Kit; Tryptophan 5-hydroxylase 1 ELISA Kit; EC 1.14.16.4 ELISA Kit; Tryptophan 5-monooxygenase 1 ELISA Kit
  • 縮寫：
    TPH1
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    37.5 pg/mL-2400 pg/mL
  • 靈敏度：
    9.4 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Neuroscience
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat TPH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 97
    Range % 94-100
    1:2 Average % 88
    Range % 83-96
    1:4 Average % 92
    Range % 87-97
    1:8 Average % 111
    Range % 106-115
  • 回收率：
    The recovery of rat TPH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 89 82-96
    EDTA plasma (n=4) 105 103-108
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    2400 1.821 1.831 1.826 1.692
    1200 1.488 1.452 1.470 1.336
    600 1.151 1.218 1.185 1.051
    300 0.854 0.897 0.876 0.742
    150 0.511 0.540 0.526 0.392
    75 0.310 0.311 0.311 0.177
    37.5 0.266 0.254 0.260 0.126
    0 0.138 0.129 0.134 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Oxidizes L-tryptophan to 5-hydroxy-l-tryptophan in the rate-determining step of serotonin biosynthesis.
  • 基因功能參考文獻(xiàn)：
    1. Ursolic acid derivatives targeted to tryptophan hydroxylase 1 prevented bone loss by suppressing serotonin biosynthesis without estrogenic side-effects in ovariectomized rats. PMID: 25930119
    2. there exists possible interrelation between tyrosine hydroxylase and TPH gene expression and epigenetic histone acetylation in chronic unpredictable stress-induced depressive rats, which at least partly contributes to the etiology of depression. PMID: 24495952
    3. An increased expression of TPH1 in the dorsal raphe nucleus may be the primary mechanism to explain the chronic increase in the brain synthesis of 5-HT in these in utero malnourished rats. PMID: 22819790
    4. Transfection of VMAT-1 and TPH-1 induced vesicular storage of serotonin which is triggered release upon cooling and has protective effects against hypothermia. PMID: 22253933
    5. An adjacent GC-rich cis -acting sequence cooperates with the inverted CCAAT box for full activation of basal transcription; both elements are essential for full cAMP response. In pinealocytes, NF-Y and Sp1 bind the inverted CCAAT box and GC-rich-region PMID: 12065627
    6. regulation of rat tryptophan hydroxylase gene expression by stress involves 5' untranslated region PMID: 12153488
    7. TPH1 mRNA expression was 105-fold more abundant in rat pineal than TPH2; TPH2 expression within the gland showed no significant variation with time of day and was very low. PMID: 12911638
    8. This study reveals adrenal glucocorticoid-independent induction of tryptophan hydroxylase gene expression in raphe nuclei in response to immobilization stress. PMID: 14744469
    9. TPH2 mRNA is expressed in raphe, in a pattern overlapping the histologically defined raphe nuclei. In sharp contrast, TPH1 (the previously known TPH) is expressed predominantly in pineal gland. PMID: 14960297
    10. Tph1 and Tph2 transcripts showed robust diurnal rhythms of abundance, with highest levels at night. PMID: 15194882
    11. Results show that the synthesis of 5-hydroxytryptamine and the expression of tryptophan hydroxylase in the dorsal raphe of rat offspring were suppressed by maternal ethanol intake. PMID: 16198203
    12. TPH1 mRNA is 2.5-fold upregulated by the stress in dorsal raphe nucleus but not in pineal gland. PMID: 18197473
    13. Data showed that calcium influx blockaded by nifedipine caused a decrease in tryptophan hydroxylase activity. PMID: 18221757
    14. mRNA for TPH-1 was present in peripheral arteries, with low levels of TPH protein and TPH activity PMID: 18414394
    15. data support the model that increased nocturnal 5-HT synthesis in the pineal gland is mediated by the phosphorylation of TPH1 at the serine 58, which elevates the TPH1 protein content and activity at night PMID: 18705647

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  • 蛋白家族：
    Biopterin-dependent aromatic amino acid hydroxylase family
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