Rat ghrelin (GHRL) ELISA kit
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中文名稱:大鼠饑餓激素(GHRL)酶聯(lián)免疫試劑盒
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貨號:CSB-E09816r
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規(guī)格:96T/48T
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價格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:
This Rat GHRL ELISA Kit was designed for the quantitative measurement of Rat GHRL protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL .
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別名:Ghrl ELISA Kit; Appetite-regulating hormone ELISA Kit; Growth hormone secretagogue ELISA Kit; Growth hormone-releasing peptide ELISA Kit; Motilin-related peptide) [Cleaved into: Ghrelin; Obestatin-23; Obestatin-13] ELISA Kit
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縮寫:
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Uniprot No.:
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種屬:Rattus norvegicus (Rat)
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樣本類型:serum, plasma, cell culture supernates
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檢測范圍:7.8 pg/mL-500 pg/mL
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靈敏度:1.95 pg/mL
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反應(yīng)時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Cardiovascular
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat GHRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:5 Average % 97 Range % 92-102 1:10 Average % 98 Range % 95-103 1:20 Average % 101 Range % 95-106 1:40 Average % 94 Range % 85-99 -
回收率:
The recovery of rat GHRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 96 90-102 EDTA plasma (n=4) 93 89-97 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 500 2.637 2.691 2.664 2.469 250 2.217 2.258 2.238 2.043 125 1.523 1.589 1.556 1.361 62.5 0.965 0.974 0.970 0.775 31.2 0.546 0.591 0.569 0.374 15.6 0.357 0.369 0.363 0.168 7.8 0.298 0.274 0.286 0.091 0 0.194 0.196 0.195 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Liujunzi Decoction ameliorated cisplatin-induced anorexia by inhibiting the JAK-STAT signaling pathway and coordinating anorexigenic and orexigenic neuropeptides in rats Y Dai,Journal of ethnopharmacology,2023
- Layer‐Specific BTX‐A Delivery to the Gastric Muscularis Achieves Effective Weight Control and Metabolic Improvement S Wang,Advanced science,2023
- Maternal acrylamide exposure changes intestinal epithelium, immunolocalization of leptin and ghrelin and their receptors, and gut barrier in weaned offspring S Muszyński,Scientific reports,2023
- Yijung-tang improves thermogenesis and reduces inflammation associated with gut microbiota in hypothyroid rats S Khakisahneh,NPJ biofilms and microbiomes,2023
- The effect of Intermittent fasting on the growth and ghrelin hormone in rats feeding on a standard diet N Günbatar,Journal of Contemporary Medicine,2022
- Sleeve Gastrectomy with Bypass of Proximal Small Intestine Provides Better Diabetes Control than Sleeve Gastrectomy Alone Under Postoperative High-Fat Diet Cheng Y.et al,Obesity Surgery,2018
- Difference of liver and kidney metabolic profiling in chronic atrophic gastritis rats between acupuncture and moxibustion treatment Qi-da He.et al,/,/
- Comparative metabolomics study on therapeutic mechanism of electro-acupuncture and moxibustion on rats with chronic atrophic gastritis (CAG) Liu CC.et al,Sci Rep,2017
相關(guān)產(chǎn)品
靶點詳情
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功能:Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation.; Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility.
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基因功能參考文獻(xiàn):
- Obestatin regulates cardiovascular function and promotes cardioprotection through the nitric oxide pathway. PMID: 28744974
- Targeted inhibition of ghrelin gene in pancreatic acinar cells of acute pancreatitis can upregulate the expression of the intracellular inflammatory factors and alleviate the intracellular calcium overload. PMID: 28984792
- Study concludes that the ghrelin receptors of the lumbosacral defecation centers have a physiological role in the control of defecation, but that their role is not dependent on ghrelin. PMID: 28801520
- Study shows that ghrelin appears to act as a modulating factor for binge-like eating behaviour by shifting food preference towards a more nutritious choice (from high fat diet to chow), with these effects being somewhat divergent from fasting. PMID: 28219000
- results reveal that ghrelin can regulate negative emotionality in unstressed animals without altering appetite. PMID: 28010876
- Findings provide evidence that ghrelin and glucagon-like peptide-1 signaling interact in the hypothalamic control of metabolic function. PMID: 27454242
- ghrelin may provide neuroprotective effect in COH retinas through activating ghrelin/GHSR-1a system, which was mediated by inhibiting retinal autophagy, ganglion cell apoptosis, and Muller cell gliosis. PMID: 29222553
- These results provide new insights into the mechanism by which ghrelin regulates voluntary activity independent of arcuate nucleus neurons. PMID: 27825986
- In order to elucidate the possible role of HO-1 in mediating the protective effects of ghrelin, tin protoporphyrin (SnPP) HO-1 blocker was administrated; it significantly attenuated the gastroprotective effect of ghrelin. In conclusion HO-1 activity significantly contributes toward ghrelin-mediated gastroprotection. PMID: 28635609
- Expression of ghrelin and ghrelin receptor and glucose stimulated insulin secretion (GSIS) of pancreatic islets. PMID: 28410130
- l-Ornithine stimulates growth hormone secretion through the sympathetic nervous and ghrelin systems. PMID: 28513740
- These findings have implications for how females may respond to stress throughout life, suggesting the way ghrelin modifies the stress response at the level of the pituitary may be less efficient in the neonatally overfed. PMID: 28282447
- Data suggest that changes in obestatin, GPR39, and ghrelin may contribute to metabolic abnormalities in T1DM, T2DM, and obesity. PMID: 27106635
- Up-regulation of miR-21 by ghrelin ameliorates ischemia/reperfusion-induced acute kidney injury by inhibiting inflammation and cell apoptosis. PMID: 27152763
- First demonstration that the stomach-produced hormone ghrelin increases impulsivity and also indicates that ghrelin can change two major components of impulsivity-motor and choice impulsivity. PMID: 26424164
- Findings show that obestatin is expressed in adult rat hippocampal progenitor cells promoting cell proliferation and preventing apoptosis. PMID: 26586206
- Ghrelin positively regulates GI motility by exciting both central and enteric neurons, including those of paraventricular nucleus by activating growth hormone secretagogue receptor & neuropeptide Y pathways & muscarinic acetylcholine receptors PMID: 26431788
- Results from this study indicate that exogenous ghrelin induces expression and activity of cardiac iNOS via Akt phosphorylation followed by NFkappaB activation in HF rats. PMID: 26600052
- These results suggest that administration of ghrelin ameliorates impaired angiogenesis in diabetic MI rats. And these beneficial effects derive from regulating GHSR1a-mediated AMPK/eNOS signal pathway PMID: 26364514
- The expression of hypothalamic ghrelin was suppressed at puberty onset. PMID: 24668712
- Central acylated ghrelin improves memory function and hippocampal AMPK activation and partly reverses the impairment of energy and glucose metabolism in rats infused with beta-amyloid. PMID: 26188171
- Because ghrelin is related to food anticipatory activity, it is plausible that ghrelin participates in the circadian rhythm of daily activity including eating behavior. PMID: 26122892
- Administration of either obestatin or ghrelin exerts similar protective effects against I/R-induced ileal and pulmonary injury, thus warranting further investigation for their possible use against ischemic intestinal injury. PMID: 26032330
- The results of the present study suggest that ghrelin secretion from isolated islets is not glucose-dependent. PMID: 26612921
- may act primarily via a central pathway within the brain to suppress sympathetic nerve activity after myocardial infarction PMID: 26121343
- Gastric ghrelin levels increased in two- and three-month diabetic rats compared to the control rats. PMID: 26467127
- The ghrelin system may play an important role regulating cardiac remodeling after MI and present as a potential significant target for pharmacological modulation and treating cardiac remodeling. PMID: 25058156
- Results suggest that ghrelin mediates a novel branch of the stress response and highlight a previously unrecognized role for ghrelin and growth hormone in maladaptive changes following prolonged stress PMID: 24126924
- Total serum ghrelin levels were significantly increased in rats with human neuroblastoma transplants and altered for those with hepatoblastoma. PMID: 25111274
- The data showed that intraportal infusion of ghrelin exerted an inhibitory effect on GLP-1 secretion through growth hormone secretagogue receptor 1alpha (GHS1alpha receptor). PMID: 25247193
- Ghrelin may exert its effects by influencing the functional status of different cell types in the gastric muscle layer to subsequently enhance the contractive effect of cholinergic neurotransmitters and enhance gastric motility. PMID: 25230765
- Acute orexigenic and hyperglycemic effects of T3 are associated with ghrelin secretion and activity. PMID: 24357139
- these results suggest that ghrelin markedly decreases IBa via the activation of GHS-R1a, which is coupled sequentially to the activities of Go-protein betagamma subunits and the downstream PKA pathway PMID: 25049077
- These results indicate the importance of peripheral obestatin in the cholinergic control of intestinal contractility in both neonatal and adult rats. PMID: 25193331
- our results demonstrate that protein kinase C delta phosphorylation at Ser/Tyr plays a critical role in the modulation of gastric mucosal inflammatory responses to H. pylori LPS by ghrelin PMID: 24840386
- ghrelin enhances the proliferation of murine CD4+ primary T cells and a CD4+ T-cell line PMID: 25447526
- Ghrelin expression was detected in the ciliary processes and GHSR-1 expression was detected in the trabecular meshwork and ciliary body. PMID: 24780389
- Ghrelin has a role in modulating fMRI BOLD response of homeostatic and hedonic brain centers regulating energy balance in male but not female rats PMID: 24830778
- Peripherally circulating ghrelin was found to not mediate alcohol-induced reward. PMID: 24428428
- Total ghrelin secretion during the 24-h period increased in the restricted feeding schedule as a result of elevation of the unacylated form PMID: 24617825
- Serum levels of ghrelin are significantly positively correlated with Ang II in chronic heart failure (CHF) patients and that ghrelin can inhibit Ang II-induced cardiomyocyte apoptosis by down-regulating AT1R, thereby playing a role in preventing HF. PMID: 24465706
- Bilateral ovariectomization leads to histological changes in the uterine tissue and decreased endometrium ghrelin immunoreactivity. PMID: 24979506
- It is mainly produced in the stomach and inhibits the neural activity of the gastric branch of the afferent vagus nerve PMID: 24366191
- Ghrelin plays an important role in promoting gastric motility. PMID: 24036593
- Ghrelin stimulates food intake and weight gain and may also induce mechanisms of cell protection that help to detour or delay systemic inflammatory responses and hypothalamic gliosis due to excess weight gain. PMID: 24848869
- Acylated ghrelin leads to earlier network formation and activation in cultured cortical neuronal networks, the latter being a possibly consequence of accelerated synaptogenesis. PMID: 24742241
- Ghrelin accelerates the healing of oral ulcers. PMID: 24304579
- data identify the VTA to NAc dopaminergic projections, along with D1-like and D2 receptors in the NAc, as essential elements of the ghrelin responsive circuits controlling food reward behavior. PMID: 23770258
- Ghrelin administration reduced ventricular arrhythmias concomitant with prevention of the loss of connexin-43 during acute myocardial infarction. PMID: 23494606
- After 2-weeks of hypoxia, erythropoietin transcripts and erythropoietin plasma levels were significantly increased in hypoxic animals compared with control animals. PMID: 24524370
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亞細(xì)胞定位:Secreted.
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蛋白家族:Motilin family
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組織特異性:Ghrelin is broadly expressed with higher expression in the stomach. Very low levels are detected in the hypothalamus, heart, lung, pancreas, intestine and adipose tissue. Obestatin is most highly expressed in jejunum, and also found in duodenum, stomach,
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