Rat hypoxia-inducible factor 1α,HIF-1α ELISA Kit
產(chǎn)品詳情
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產(chǎn)品描述:
This Rat HIF1A ELISA Kit was designed for the quantitative measurement of Rat HIF1A protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL .
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別名:
Hif1aHypoxia-inducible factor 1-alpha ELISA Kit; HIF-1-alpha ELISA Kit; HIF1-alpha ELISA Kit
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縮寫:
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Uniprot No.:
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種屬:
Rattus norvegicus (Rat)
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樣本類型:
serum, plasma, tissue homogenates
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檢測范圍:
3.12 pg/mL-200 pg/mL
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靈敏度:
0.78 pg/mL
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反應(yīng)時間:
1-5h
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樣本體積:
50-100ul
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檢測波長:
450 nm
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研究領(lǐng)域:
Epigenetics and Nuclear Signaling
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測定原理:
quantitative
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測定方法:
Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | | | |
Three samples of known concentration were tested twenty times on one plate to assess. | |
Inter-assay Precision (Precision between assays): CV%<10% | | | |
Three samples of known concentration were tested in twenty assays to assess. | | |
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線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat HIF-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
1:1 | Average % | 90 | |
Range % | 87-94 | |
1:2 | Average % | 96 | |
Range % | 91-100 | |
1:4 | Average % | 93 | |
Range % | 87-97 | |
1:8 | Average % | 100 | |
Range % | 96-103 | |
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回收率:
The recovery of rat HIF-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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Sample Type | Average % Recovery | Range | |
Serum (n=5) | 92 | 87-95 | |
EDTA plasma (n=4) | 95 | 88-98 | |
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標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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pg/ml | OD1 | OD2 | Average | Corrected | | 200 | 2.715 | 2.656 | 2.686 | 2.538 | | 100 | 1.730 | 1.676 | 1.703 | 1.555 | | 50 | 1.196 | 1.179 | 1.188 | 1.040 | | 25 | 0.608 | 0.618 | 0.613 | 0.465 | | 12.5 | 0.404 | 0.412 | 0.408 | 0.260 | | 6.25 | 0.303 | 0.316 | 0.310 | 0.162 | | 3.12 | 0.248 | 0.237 | 0.243 | 0.095 | | 0 | 0.150 | 0.146 | 0.148 | | |
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本試劑盒所含材料:
- A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-Rat HIF-1α antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled HIF-1α antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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本試劑盒不含材料:
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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數(shù)據(jù)處理:
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貨期:
3-5 working days
引用文獻(xiàn)
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Boosting effects of Cranberry and Cinnamaldehyde for pioglitazone amelioration of liver steatosis in rat via suppression of HIF-1α/Smad/β-catenin signaling
MA Ali,Journal of Functional Foods,2023
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Oxygen-Glucose Deprivation in Organotypic Hippocampal Cultures Leads to Cytoskeleton Rearrangement and Immune Activation: Link to the Potential Pathomechanism of Ischaemic Stroke
N Bryniarska-Kubiak,Cells,2023
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Cubic multi-ions-doped Na2TiO3 nanorod-like coatings: Structure-stable, highly efficient platform for ions-exchanged release to immunomodulatory promotion on vascularized bone apposition
Dongmei Yu,Bioactive Materials,2022
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Assessment of the effects of ischaemia/hypoxia on angiogenesis in rat myofascial trigger points using colour Doppler flow imaging
F Jiang,PeerJ,2020
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Short-term exercise training improves cardiac function associated to a better antioxidant response and lower type 3 iodothyronine deiodinase activity after myocardial infarction
Marschner RA, et al,Plos One,2019
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A Promising Approach to Integrally Evaluate the Disease Outcome of Cerebral Ischemic Rats Based on Multiple-Biomarker Crosstalk
Guimei Ran.et al,Disease Markers,2017
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Expression and specific activities of carbamoyl phosphate synthetase 1 in chronic hypoxic rats
Nikmah U A.et al,Medical Journal of Indonesia,2016
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Measurement of levels of lung HIF-1偽 protein in response to tapering for 14- and 21-day with nigella sativa supplementation in maturing rat, with histological study
Ehsan Arabzadeh.et al,Sport Sciences for Health,2015
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Influence of thyroid state on cardiac and renal capillary density and glomerular morphology in rats
Rodríguez-Gómez I et al,J Endocrinol,2013
靶點(diǎn)詳情
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功能:
Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with NCOA1 and/or NCOA2. Interaction with redox regulatory protein APEX1 seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.
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基因功能參考文獻(xiàn):
- Serum level of cardiac biomarkers and the necrotic areas were significantly lower in the Ad-HIF-1a-Trip group compared to the other groups in myocardial infarction. Cardiac function in the Ad-HIF-1alpha-Trip group was decreased less profoundly through day 7 to day 28 compared to the other groups. PMID: 30423574
- the interplay between HIF1alpha and ROCK1 may be a critical factor that regulates cell proliferation and collagen synthesis in rat hepatic stellate cells under hypoxia. PMID: 30132575
- Early changes in impedance in response to I/R is related to histopathological changes, the nuclear stabilization and translocation of HIF-1alpha as well as expression of iNOS. PMID: 29451295
- Stud shows that HIF-1alpha induction during reperfusion is a protector mechanism implicated in a normal renal tissue repair after ischemia/reperfusion. PMID: 28106131
- HRS reduced mechanical hyperalgesia and activation of cell autophagy in neuropathic pain through a HIF1-dependent pathway. PMID: 29888265
- Succinate acts as a signaling molecule in hypoxic stabilization and HIF-1alpha expression induction in the brain cortex. PMID: 29308570
- pathologic hypoxia stimulates neural stem cells proliferation by increasing expression of HIF-1alpha and activating the Wnt/beta-catenin signaling pathway. PMID: 28838333
- These results indicate that HIF-1alpha expression can be induced and activated in rats during the acute lung inflammatory damages triggered by septic lymph injection and that lung inflammatory injuries occur via a HIF-1alpha-dependent pathway. PMID: 28478272
- EC-mediated protection is only possible when there is a bidirectional crosstalk between ECs and CMs even without physical cell-cell contact. In addition, this protective effect is at least partially related to cell-ECM interactions and HIF-1alpha, which is regulated by HIF1A-AS1 under oxidative stress. PMID: 28629892
- Data suggest that overexpression of hypoxia inducible factor 1 alpha subunit (HIF-1alpha) by gene therapy may be a useful method for enhancing alveolar bone defect osteogenesis and angiogenesis. PMID: 26929250
- By using a cell model of desferoxamine-dependent HIF activation, we demonstrated that propofol was able to inhibit apoptosis and mitochondrial depolarization associated to HIF-1a action. In conclusion, hepatic I/R injury induces mitochondrial dysfunction that is not prevented by inhaled sevoflurane. PMID: 27154980
- Hypoxia and hyperoxia differentially control proliferation of rat neural crest stem cells via distinct regulatory pathways of the HIF1alpha-CXCR4 and TP53-TPM1 proteins PMID: 28002632
- HDACs controlled HIF-1alpha stability by regulating HIF-1alpha-PHD2 interaction in NP cells. PMID: 26765925
- The GO-Cu nanocomposites upregulate the expression of Hif-1alpha in BMSCs. PMID: 26945787
- Impaired ischemia-induced angiogenesis in rats with chronic kidney disease can be improved by HIF stabilization, even if started after onset of ischemia. PMID: 27927598
- Warburg effect plays a critical role in PDGF-induced pulmonary arterial smooth muscle cells proliferation and is mediated by activation of the PI3K signaling pathway and HIF-1alpha. PMID: 28738389
- Work identifies HIF-1 as an early chronic kidney disease (CKD)-related pathological event, prospective marker, and potential target against vascular calcification in CKD-relevant conditions. PMID: 27470678
- High HIF1A expression is associated with inflammation and fibrosis in kidney. PMID: 26891083
- Hypoxia-inducible factor 1alpha knockdown could be a potential approach to improve the efficacy of temozolomide therapy for pituitary adenomas. PMID: 26647409
- Report significant impairment of renal oxygenation and mitochondrial function at the early stages of chronic kidney disease and demonstrate the beneficial role of HIF-1alpha activation on renal function and metabolism. PMID: 28331062
- We show that HACT requires transcriptional activation of HIF-1alpha throughout the course of HA and that this activation is accompanied by two metabolic switches. PMID: 28274939
- In response to hypoxia, compared with rats, mice had higher minute ventilation, lower metabolic rate and higher expression of HIF-1alpha in the brainstem. PMID: 27742895
- Can use light microscopy to separate renal cells into those with and without HIF1alpha expression. PMID: 28212880
- HIF-1alpha activation via cytosolic Ca(2+) signaling pathway play a role in the mechanism of isoflurane-induced neurodegeneration in neonatal rodents, suggesting HIF-1alpha as a potential therapeutic target for the prevention of the deleterious effects of prolonged exposures to anesthetics. PMID: 27769790
- Immunohistochemistry staining, mRNA, and protein for hypoxia-inducible factor 1alpha were upregulated within the paraventricular nucleus of left coronary artery-ligated chronic heart failure rats. PMID: 27810863
- The expression of three well-defined downstream targets of hypoxia inducible factor 1 alpha subunit (HIF-1alpha), pyruvate dehydrogenase kinase 1 (PDK1), lactate dehydrogenase A (LDHA) and vascular endothelial growth factor (VEGF), were up-regulated in hippocampus after 28 days of simulated microgravity exposure. PMID: 27988334
- The mechanism of development of liver fibrosis involves activation of matrix metalloproteinase-2 and matrix metalloproteinase-9, up-regulation of HIF-1alpha transcriptional activity and its related factors, NF-kappaB and TGF-beta. PMID: 27260943
- Reduction of endoplasmic reticulum stress by 4-phenylbutyric acid reduces chronic hypoxia induced right ventricular hypertrophy and improves hypoxic tolerance through upregulation of HIF-1alpha. PMID: 27058435
- Nox2 is a modulator of CsA-induced hypoxia upstream of HIF-1alpha PMID: 26950727
- The results demonstrate that inflammation-induced fetal growth restriction is associated with increased placental HIF-1alpha accumulation; however, expression of this transcription factor may not correlate with regions of hypoxia in late-gestation placentas. PMID: 28423052
- this study demonstrates the potent suppressive effects of HIF-1alpha shRNA on hypoxia-induced PH and PASMC hyperplasia, providing evidence for the potential application of HIF-1alpha shRNA in the treatment of hypoxic PH. PMID: 27748831
- The present experiments indicated that IQ enhanced the radiosensitivity of C6 rat glioma cells both in vitro and in vivo. The primary mechanism of this radiosensitizing effect involves the downregulation of HIF-1alpha. F-FDG and F-FMISO PET/CT were sensitive and noninvasive for monitoring the early radiosensitizing effect of IQ. Meanwhile, F-FMISO PET/CT provided more information on the changes in tumor hypoxic status. PMID: 26963468
- Stabilization of HIF-1alpha by inhibiting HIF-1alpha hydroxylation prevented the ischemic decrease in both PMCA1 and PMCA2 mRNAs in CA1 neurons PMID: 26969192
- Data show that cardiac arrest (CA) increased HIF-1alpha and pro-inflammatory cytokines (PICs) including IL-6 and TNF-alpha in the hippocampus CA1 region. PMID: 27383646
- specific signaling pathways engaged in the development of diabetic retinopathy, including the activation of mTOR and HIF-1alpha -VEGF mechanism, were investigated. PMID: 27997905
- Hypoxia could cause HIF-1alpha-dependent increase in the expression of p27 leading to cell cycle arrest in G0/G1 phase PMID: 26920732
- hypoxia or GSN overexpression induces HIF-1alpha expression and reduces the expression of survival markers p-Akt and Bcl-2 in H9c2 cardiomyoblast cells PMID: 27193608
- HIF-1A regulates epithelial-mesenchymal transition via the Snail and beta-catenin pathways in paraquat poisoning-induced early pulmonary fibrosis. PMID: 26781174
- Data show that under hypoxia, sirtuin 1 (SIRT1) expression was raised and matrix metalloproteinase-9 (MMP-9) and hypoxia inducible factor 1alpha (HIF-1alpha) were reduced by Lycium barbarum polysaccharides (LBP) in a dose-dependent manner. PMID: 27363270
- tumor necrosis factor tumor necrosis factor cachectin tumor necrosis factor (TNF superfamily member 2) tumor necrosis factor alpha tumor necrosis factor ligand superfamily member 2 PMID: 26614261
- Chronic intermittent hypobaric hypoxia has renal protective effects in diabetic rats, which might be related with activating HIF1alpha signaling. PMID: 27351799
- shRNA of HIF-1alpha could antagonize the hypoxia-mediated increase in hepatic cancer cell migration and invasion, and synergize with hypoxia to inhibit the cell proliferation in HCC cells PMID: 25388848
- reduced miR-195 expression partially protects rats from spinal cord injury, primarily by targeting HIF-1alpha. PMID: 26927342
- 24 h after hypoxic-ischemic injury, there was a significant increase in PHD3 protein and an increase of HIF-1alpha compared to controls PMID: 26108712
- nano-microcapsule bFGF combined with HIF-1 prevented random skin flap survival in rats through antioxidative, anti-inflammatory and activation of the protein expression levels of COX-2 and VEGF. PMID: 26707180
- Study suggested that neonatal bronchopulmonary dysplasia caused impaired cognitive function and neuron apoptosis in hippocampus via p53 and HIF-1alpha PMID: 26431790
- Coupled with HIF-1alpha and vascular endothelial growth factor (VEGF). PMID: 26537366
- expression of VEGF and HIF-1alpha might be involved with myocardial remodeling and angiogenesis in myocardial infarction PMID: 26813461
- HIF1alphamediated expression of VEGF may be one of the important mechanisms regulating ovarian luteal development in mammals in vivo. PMID: 26323652
- The hypoxia-induced HIF-1alpha could promote glucose metabolism to protect hepatocellular mitochondria from damage. It could be a useful way to protect liver against I/R injuries and inflammatory injury. PMID: 25726501
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亞細(xì)胞定位:
Cytoplasm. Nucleus. Nucleus speckle.
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組織特異性:
Expressed in the kidney, higher expression is seen in the renal medulla than in the cortex. Expressed also in the perivenous zone of the liver.
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