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  4. Rat neutrophil gelatinase-associated lipocalin,NGAL ELISA Kit

Rat neutrophil gelatinase-associated lipocalin,NGAL ELISA Kit

  • 中文名稱：
    大鼠中性粒細胞明膠酶相關脂質(zhì)運載蛋白(NGAL)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E09409r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
        LCN2 (Lipocalin-2), 又稱NGAL (Neutrophil Gelatinase-Associated Lipocalin)，是一種由各種細胞產(chǎn)生的蛋白質(zhì)，如肝細胞、腎小管上皮細胞、巨噬細胞和一些腫瘤細胞。LCN2可作為促炎性細胞因子，具有在免疫系統(tǒng)中發(fā)揮重要作用的抗菌和抗病毒活性。在腎臟損傷、心血管疾病和某些腫瘤中，LCN2的水平會顯著升高，因此它也被用作這些疾病的生物標志物。
        華美生物所提供的Rat neutrophil gelatinase-associated lipocalin,NGAL ELISA Kit屬于ELISA檢測試劑盒，采用雙抗夾心法定量檢測大鼠血清、血漿、組織勻漿樣本中的LCN2，其靈敏度為0.078 ng/ml，檢測范圍為0.3125 ng/ml- 20ng/ml。
     
  • 別名：
    Lcn2 ELISA Kit; Neutrophil gelatinase-associated lipocalin ELISA Kit; NGAL ELISA Kit; Alpha-2-microglobulin-related protein ELISA Kit; Alpha-2U globulin-related protein ELISA Kit; Lipocalin 24p3 ELISA Kit; Lipocalin-2 ELISA Kit; Siderocalin LCN2 ELISA Kit; p25 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.3125 ng/ml- 20ng/ml
  • 靈敏度：
    0.078 ng/ml
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 本試劑盒所含材料：
      • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-rat NGAL. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial Biotin-labeled NGAL antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
      • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
      • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
      • One vial Sample Diluent(50 ml/bottle)---Dilute the sample to an appropriate concentration.
      • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
      • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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  • 本試劑盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻

產(chǎn)品評價

靶點詳情

  • 最新研究進展：
    LCN2（鯨藍蛋白2）是一種鐵載體蛋白，廣泛存在于哺乳動物的體液和細胞中。最近的研究表明，LCN2在多種疾病的發(fā)生發(fā)展中發(fā)揮重要作用，如腫瘤、心血管疾病、炎癥性疾病等。此外，LCN2還可能參與多種生理過程，如細胞凋亡、鐵代謝等。
  • 功能：
    Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,3-dihydroxybenzoic acid (2,3-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity; limits bacterial proliferation by sequestering iron bound to microbial siderophores, such as enterobactin. Can also bind siderophores from M.tuberculosis.
  • 基因功能參考文獻：
    1. NGAL might be related to attenuated tubular epithelial cell damage via adjusting autophagy. PMID: 29367586
    2. The results showed that remote limb preconditioning could decrease the expression of LCN2 protein, but having no obvious effects on its receptor except the time point of 72h after cerebral ischemia. PMID: 29122651
    3. High LCN2 expression is associated with stress-induced mitochondrial dysfunction and heart failure. PMID: 29378951
    4. This study is to investigate the level of Neutrophil gelatinase-associated lipocalin (NGAL) and autophagy status during renal Ischemia/reperfusion injury. PMID: 29704511
    5. This study demonstrated that Neutrophil gelatinase-associated lipocalin and microglial activity are associated with distinct postoperative behavioral changes in rats. PMID: 27865916
    6. LCN-2 protein levels were markedly increased in the ipsilateral cortex and hippocampus after traumatic brain injury. Most LCN-2-positive cells appeared to be astrocytes. PMID: 26463963
    7. Nitric oxide level affects LCN-2 expression via regulation of LCN-2 protein stability under inflammatory condition and LCN-2 may reduce beta-cell viability by promoting apoptosis. PMID: 27233602
    8. The NGAL molecule showed upregulation in the degeneration process after nerve injury, so it may play an important role in nerve repair PMID: 27087673
    9. The LCN2 may be a potentially useful biomarker for lung hypoplasia in a rat and human Congenital diaphragmatic hernia (CDH). PMID: 27592368
    10. Hypertension and hyperglycemia synergize to cause incipient renal tubular alterations resulting in increased NGAL urinary excretion.NGAL urinary excretion is also increased in hypertensive-diabetic rats. PMID: 25148248
    11. This is the first report on Pla2g4c gene-deficient rats and their low susceptibility to mammary carcinogenesis by enhancing NF-kappaB/Lcn2-induced apoptosis. PMID: 26013918
    12. Epidural anesthesia was associated with an increase in the expression lipocalin-2 and a decrease in the expression of E coli DNA at pseudosurgical sites in sick but not healthy rats. PMID: 25782996
    13. Repeated administration of gentamicin to male SD rats for 1, 3, or 7 days resulted in a dose- and time-dependent increase in the expression of KIM-1 and NGAL. PMID: 25087119
    14. dietary fructose leads to an overexpression of lipocalin-2 in rat fatty liver PMID: 24587658
    15. Lcn2 is an important regulator in small-for-size fatty liver graft injury and targeting Lcn2 may be feasible for preventing marginal graft failure in LDLT. PMID: 24374540
    16. NGF-treated tissue showed increased expression of LCN2 which was inhibited by the presence of Ro 08-2750 in intervertebral disk cells. PMID: 25062286
    17. lipocalin-2 is released by injured neurons as a help me distress signal that activates microglia and astrocytes into potentially prorecovery phenotypes. PMID: 24916903
    18. r24p3-R/hNGAL-R may represent a high-affinity multi-ligand receptor for apical internalization and transcytosis of intact proteins/peptides by the lower intestine. PMID: 23940770
    19. Urinary KIM-1, NGAL and NAG were compared against serum creatinine and renal histological score in the initiation, maintenance, and recovery phases of cisplatin (CP)-induced acute kidney injury. PMID: 23360846
    20. We studied Lcn2 gene expression during liver regeneration after partial hepatectomy(PH). Lcn2 gene expression showed upward curves from 4 to 72 h after PH in both 70% and 40% PH rats and peaked at 12 h (8 times vs 0 h). PMID: 23481292
    21. The expression of NGAL significantly increases in renal interstitial fibrosis, and is closely correlated with MMP-9, TNF-alpha and TGF-beta1. PMID: 23158800
    22. lcn2 is induced in activated astrocytes in response to neurodegeneration, but its induction is independent of TDP-43 or FUS expression in astrocytes PMID: 23431168
    23. High NGAL is associated with proteinuria. PMID: 21551085
    24. Acute myocardial infarct in the chronic kidney disease setting is associated with accelerated renal fibrosis and long-term elevated urine Ngal values. PMID: 22889806
    25. Exposure of Wistar rats to methyl tertiary-butyl ether in drinking water resulted in limited renal changes in male rats suggestive of alpha(2u)-globulin nephropathy. PMID: 22833177
    26. Adoptive transfer of IL-10 macrophages to ischemic kidneys blunts acute kidney injury through induction of lipocalin-2. PMID: 22278021
    27. LIPOCALIN-2 is a major acute-phase protein in a rat and mouse model of sterile abscess PMID: 22249220
    28. Lcn2-induced cardiomyocyte apoptosis is of physiological significance and occurs via a mechanism involving elevated intracellular iron levels and Bax translocation. PMID: 22117066
    29. An evaluation of the SIP24 and MPO distribution and levels of expression in the rat uterus is consistent with the hypothesis that SIP24 modulates the neutrophil population by activities such as inducing their apoptosis. PMID: 16546827
    30. SMC-produced NGAL interacts with MMP-9, a mechanism by which NGAL may modulate MMP-9 proteolytic activity in the vascular repair process.[Neutrophil gelatinase-associated lipocalin, NGAL] PMID: 17148685
    31. LCN2 expression shows clear correlation to liver damage and resulting inflammatory responses, rather than to the degree of liver fibrosis, which in fact may imply a distinct diagnostic value as an early biomarker of liver inflammation. PMID: 21457438
    32. Cardiomyocytes, vascular wall cells and fibroblasts in myocarditis strongly express Lcn2/NGAL via proinflammatory cytokines PMID: 20057160
    33. Acute phase response to acute endotoxemia involves induction of Lcn2 in both the lung and liver. PMID: 17490638

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  • 亞細胞定位：
    Secreted. Cytoplasmic granule lumen. Cytoplasmic vesicle lumen.
  • 蛋白家族：
    Calycin superfamily, Lipocalin family
  • 組織特異性：
    Detected in the ureteric bud in embryonic kidney (at protein level).
  • 數(shù)據(jù)庫鏈接：