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Rat oxyntomodulin ELISA Kit

  • 中文名稱：
    大鼠胃泌酸調(diào)節(jié)素酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E12949r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    This Rat OXM ELISA Kit was designed for the quantitative measurement of Rat OXM protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 125 pg/mL-8000 pg/mL and the sensitivity is 31.25 pg/mL .

  • 縮寫：
    OXM
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    125 pg/mL-8000 pg/mL
  • 靈敏度：
    31.25 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat oxyntomodulin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %86
    Range %81-92
    1:2Average %96
    Range %90-102
    1:4Average %97
    Range %92-104
    1:8Average %93
    Range %90-96
  • 回收率：
    The recovery of rat oxyntomodulin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9387-100
    EDTA plasma (n=4)9995-104
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    80002.356 2.378 2.367 2.252
    40002.121 2.161 2.141 2.026
    20001.757 1.798 1.778 1.663
    10001.343 1.391 1.367 1.252
    5000.851 0.899 0.875 0.760
    2500.502 0.539 0.521 0.406
    1250.272 0.291 0.282 0.167
    00.114 0.116 0.115 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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