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中文名稱:綿羊白蛋白酶聯(lián)免疫試劑盒
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貨號:CSB-E15839Sh
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規(guī)格:96T/48T
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價格:¥4200/¥3000
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:白蛋白(Albumin)是由肝臟合成并在血液中廣泛存在的蛋白質(zhì)。它的分子量較大,結(jié)構(gòu)穩(wěn)定,具有多種生物學(xué)功能。主要功能是維持血液的滲透壓和血容量,調(diào)節(jié)離子和營養(yǎng)物質(zhì)的運輸,并參與藥物代謝和免疫調(diào)節(jié)。此外,白蛋白還有助于細胞和組織的修復(fù)和再生。由于其豐富的生物學(xué)功能和廣泛的應(yīng)用價值,白蛋白已成為醫(yī)學(xué)和生物科學(xué)研究中不可或缺的重要組成部分。
華美生物所提供的Sheep Albumin (Alb)ELISA Kit屬于ELISA檢測試劑盒,采用競爭法定量檢測綿羊血清、血漿、細胞培養(yǎng)上清,尿液、組織勻漿樣本中的ALB,其靈敏度為5.5 μg/mL,檢測范圍為350 μg/mL-21.88 μg/mL。 -
別名:ALBSerum albumin ELISA Kit
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縮寫:
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Uniprot No.:
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種屬:Ovis aries (Sheep)
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樣本類型:serum, plasma, cell culture supernates, urine, tissue homogenates
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檢測范圍:5.47 μg/ml-350 μg/ml
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靈敏度:5.47 μg/ml
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反應(yīng)時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Metabolism
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測定原理:quantitative
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測定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<12% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess.
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線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of sheep albumin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:100 Average % 92 Range % 97-101 1:200 Average % 95 Range % 88-105 1:400 Average % 95 Range % 90-100 1:800 Average % 95 Range % 92-97 -
回收率:
The recovery of sheep albumin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 87-102 EDTA plasma (n=4) 97 93-105 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. μg/ml OD1 OD2 Average 350 0.162 0.166 0.164 175 0.281 0.274 0.278 87.5 0.436 0.445 0.441 43.75 0.655 0.667 0.661 21.88 0.923 0.907 0.915 0 2.052 1.996 2.024 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻
- Estimating winter energy balance and actual changes in nutrition of Mongolian grazing sheep Y Yoshihara,Applied Animal Science,2021
相關(guān)產(chǎn)品
靶點詳情
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最新研究進展:白蛋白(ALB)是血液中最豐富的蛋白質(zhì)之一,它不僅具有多種重要的生理功能,如維持血容量和血壓、輸送藥物和其他生物分子等,而且還被廣泛應(yīng)用于臨床診斷和治療。最近的研究表明,ALB在多種疾病中具有重要作用。例如,在COVID-19疫情中,患者的ALB水平下降通常與嚴(yán)重程度呈正相關(guān),因此ALB可能是預(yù)測疾病嚴(yán)重程度和預(yù)后的重要指標(biāo)。此外,在腎病和癌癥等疾病中,ALB水平也被證明與疾病的發(fā)生和進展密切相關(guān)。例如,ALB的下降可能導(dǎo)致腎臟損傷和慢性腎臟病的發(fā)展,并被用作評估腎臟健康的重要指標(biāo);同時,ALB的降低也被發(fā)現(xiàn)與癌癥的發(fā)生和惡化有關(guān),因此可能作為腫瘤預(yù)后評估的潛在生物標(biāo)志物之一。此外,ALB水平還可以用于評估營養(yǎng)狀況,其下降通常與營養(yǎng)不良相關(guān)。綜上所述,ALB是一個多功能的蛋白質(zhì),在不同的疾病中發(fā)揮著重要的作用,有望成為評估患者健康狀況和預(yù)測預(yù)后的重要指標(biāo)。
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功能:Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma. Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner. The shared binding site between zinc and calcium at residue Asp-272 suggests a crosstalk between zinc and calcium transport in the blood. The rank order of affinity is zinc > calcium > magnesium. Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli. Does not prevent iron uptake by the bacterial siderophore aerobactin.
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基因功能參考文獻:
- We have purified SSA from sheep kidneys by a simple and efficient two-step purification procedure. Further, we have studied urea-induced denaturation of SSA by monitoring changes in the difference absorption coefficient at 287nm intrinsic fluorescence emission intensity and mean residue ellipticity at 222nm at pH 7.4 and 25 degrees C PMID: 27180298
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亞細胞定位:Secreted.
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蛋白家族:ALB/AFP/VDB family
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組織特異性:Plasma.
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數(shù)據(jù)庫鏈接:
KEGG: oas:443393
UniGene: Oar.449
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