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  4. Sheep Interleukin 10,IL-10 ELISA Kit

Sheep Interleukin 10,IL-10 ELISA Kit

  • 中文名稱：
    綿羊白介素10(IL-10)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E12817Sh
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    This Sheep IL10 ELISA Kit was designed for the quantitative measurement of Sheep IL10 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 5 pg/mL-80 pg/mL and the sensitivity is 5 pg/mL.

  • 別名：
    IL10Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Ovis aries (Sheep)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    5 pg/mL-80 pg/mL
  • 靈敏度：
    5 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 87-98  
    1:2 Average % 98  
    Range % 93-104  
    1:4 Average % 84  
    Range % 82-88  
    1:8 Average % 90  
    Range % 85-97  
  • 回收率：
    The recovery of sheep IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 91-99  
    EDTA plasma (n=4) 92 87-97  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    80 0.202 0.196 0.199    
    40 0.365 0.345 0.355    
    20 0.623 0.641 0.632    
    10 1.147 1.168 1.158    
    5 2.344 2.189 2.267    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation. In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling.
  • 基因功能參考文獻(xiàn)：
    1. The aim of the study was to assess the prognostic significance of Tumor Necrosis Factor-alpha, Interleukin-12(p40), Interleukin-6 and Interleukin 10 levels in cerebrospinal fluid (CSF) in sheep with encephalitic listeriosis. PMID: 25817420
    2. Significant differences in mitogen-specific lymphocyte proliferation in vitro and in expression of TGF-beta1 and IL-10 genes in hepatic lymph nodes were observed at acute and chronic phases of infection, respectively. PMID: 19751473
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    IL-10 family
  • 數(shù)據(jù)庫鏈接：

    KEGG: oas:443342

    UniGene: Oar.528