Your Good Partner in Biology Research

  1. 首頁
  2. 活性蛋白
  3. 生長因子
  4. Recombinant Human Thrombopoietin (THPO) (Active)

Recombinant Human Thrombopoietin (THPO) (Active)

In Stock
  • 中文名稱：
    人THPO重組蛋白
  • 貨號：
    CSB-AP003971HU
  • 規(guī)格：
    ￥1740
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 95% as determined by SDS-PAGE.
  • 內(nèi)毒素：
    Less than 0.01 EU/µg as determined by LAL method.
  • 生物活性：
    The ED50 as determined in a cell proliferation assay using MO7E human megakaryocytic leukemic cells is 0.55 ng/ml.
  • 基因名：
  • Uniprot No.：
  • 別名：
    C mpl ligand; C-mpl ligand; Megakaryocyte colony stimulating factor; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; Megakaryocyte stimulating factor; MGC163194; MGDF; MKCSF ; ML; MPL ligand; MPLLG; Myeloproliferative leukemia virus oncogene ligand; Prepro thrombopoietin; THCYT1; THPO; Thrombopoietin; Thrombopoietin nirs variant 1; TPO; TPO_HUMAN
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Full Length of Mature Protein
  • 來源：
    Mammalian cell
  • 分子量：
    37.3 kDa
  • 表達(dá)區(qū)域：
    22-353aa
  • 氨基酸序列
    SPAPPACDLRVLSKLLRDSHVLHSRLSQCPEVHPLPTPVLLPAVDFSLGEWKTQMEETKAQDILGAVTLLLEGVMAARGQLGPTCLSSLLGQLSGQVRLLLGALQSLLGTQLPPQGRTTAHKDPNAIFLSFQHLLRGKVRFLMLVGGSTLCVRRAPPTTAVPSRTSLVLTLNELPNRTSGLLETNFTASARTTGSGLLKWQQGFRAKIPGLLNQTSRSLDQIPGYLNRIHELLNGTRGLFPGPSRRTLGAPDISSGTSDTGSLPPNLQPGYSPSPTHPPTGQYTLFPLPPTLPTPVVQLHPLLPDPSAPTPTPTSPLLNTSYTHSQNLSQEG
  • 蛋白標(biāo)簽：
    N-terminal 6xHis-tagged and C-terminal 6xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    Lyophilized from a 0.2 μm Filtered 20 mM Tris-HCl, 150 mM NaCl, pH 8.0
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.
  • 基因功能參考文獻(xiàn)：
    1. The expression of TPO and c-Mpl was significantly decreased in the cITP group compared to the nITP group, suggesting that TPO and its receptor may play important roles in childhood cITP pathogenesis. PMID: 29313460
    2. FISH study showed no cytogenetic abnormalities in any of the analyzed cases. PMID: 16682284
    3. results confirm that TPO acts as an acute phase protein but exclude the possibility that it is uniquely responsible for thrombocytosis of inflammatory disorders, which might recognize in IL-6 a credible candidate as a cooperating factor. PMID: 18041648
    4. evidence for the presence of a low TPO gene expression transcript in B-CLL cells; early B-cell CLL circulating level of TPO does not provide a useful insight into the complex interrelationship of prognostic variables PMID: 18203013
    5. Thrombopoietin levels are increased in patients with severe acute respiratory syndrome; TPO may have a role in thrombocytosis, which frequently develops from thrombocytopenia in SARS patients PMID: 18314161
    6. separate binding sites on the Mpl receptor for TPO and hNUDC identified PMID: 20529857
    7. Tensin2 is an important new mediator in TPO/c-Mpl pathway; Tensin2 becomes phosphorylated in a TPO dependent manner. PMID: 21527831
    8. perioperative TPO dynamics are associated with postoperative LD. Postoperative TPO levels were found to be lowest in high-risk patients (HCC patients undergoing major resection) but showed an independent predictive value. PMID: 25611592
    9. Data indicate thrombopoietin (TPO) as potential early prognostic biomarker in acute pancreatitis (AP) patients. PMID: 28079612
    10. These studies demonstrate that biallelic loss-of-function mutations in THPO cause bone marrow failure, which is unresponsive to transplant due to a hematopoietic cell-extrinsic mechanism. PMID: 28559357
    11. Genetically engineered mesenchymal stromal cells produce IL-3 and TPO to further improve human scaffold-based xenograft models PMID: 28456746
    12. High Thrombopoietin expression is associated with immune thrombocytopenia in pregnancy. PMID: 26840092
    13. Colorectal cancer tumor-initiating cells (TICs) expressing CD110, the thrombopoietin (TPO)-binding receptor, mediate liver metastasis. We show that TPO promotes metastasis of CD110+ TICs to the liver by activating lysine degradation. PMID: 26140605
    14. decreased TPO levels or decreased bone marrow production of platelets may not be a cause of thrombocytopenia in chronic hepatitis C PMID: 25728497
    15. WASP, RUNX1, and ANKRD26 genes are important for normal TPO signaling and the network underlying thrombopoiesis. PMID: 26175287
    16. Data suggest that elevated serum level of thrombopoietin may serve as unfavorable marker of stage of multiple myeloma. PMID: 25323752
    17. The regulation of OCs by TPO highlights a novel therapeutic target for bone loss diseases and may be important to consider in the numerous hematologic disorders associated with alterations in TPO/c-mpl signaling PMID: 25656774
    18. TPO was greatly enhanced in HPT in comparison with ITP patients (958 +/- 659 and 11 +/- 27 pg/ml, p < 0.001). In the ITP group a reverse correlation was detected between TPO and glycocalicin (r = -0.373, p = 0.006). PMID: 25472766
    19. observations suggest that NRP-1 is involved in megakaryocytopoiesis through complex formation with PDGFRs, and that NRP-1-PDGFR-complexes may contribute to effective cellular functions mediated by TPO and PDGF in megakaryocytic cells PMID: 25744030
    20. An Arg->Cys substitution at residue 38 or residue 17 excluding the 21-AA signal peptide of the receptor binding domain was found in a family with aplastic anemia. Adding a 5th cysteine may disrupt normal disulfide bonding & receptor binding. PMID: 24085763
    21. increased plasma thrombopoietin levels were associated with a favorable prognosis of bone marrow failure and could, therefore, represent a reliable marker for a benign subset of myelodysplastic syndrome. PMID: 23403320
    22. Increased TPO levels may increase both platelet count and platelet size, resulting in more hemostatic tendency, which may contribute to the progression of ischemic stroke. PMID: 22327824
    23. Platelet count and serum thrombopoietin level as predictors for morbidity and/or mortality in thrombocytopenic neonates. PMID: 22980223
    24. Data indicate increased levels of serum thrombopoietin (TPO) were found in necrotizing pancreatitis. PMID: 22698803
    25. Mutation in the THPO gene is not associated with aplastic anaemia in Japanese children. PMID: 22686250
    26. Thrombopoietin is a biomarker and mediator of cardiovascular damage in critical diseases [review] PMID: 22577249
    27. Findings establish that Clock regulates Thpo and Mpl expression in vivo, and demonstrate an important link between the body's circadian timing mechanisms and megakaryopoiesis. PMID: 22284746
    28. Overstimulation of the THPO pathway might therefore predispose to clonal hematopoietic disease and to congenital abnormalities. PMID: 22453305
    29. Data show that serum thrombopoietin levels in the affected family members were significantly higher than in the non-affected family members or healthy controls. PMID: 22194398
    30. pattern of megakaryocytopoiesis is associated with up-regulated thrombopoietin (TPO) signaling through mammalian target of rapamycin (mTOR) and elevated levels of full-length GATA-1 and its targets PMID: 21304100
    31. Human thrombopoietin knockin mice efficiently support human hematopoiesis in vivo. PMID: 21262827
    32. Findings thus suggest that decreased thrombopoietin production accompanying liver dysfunction may be related to thrombocytopenia besides myelosuppression in anorexia nervosa with malnutrition. PMID: 19810087
    33. TPO negatively modulates cardiac inotropy in vitro and contributes to the myocardial depressing activity of septic shock serum PMID: 20467749
    34. Overexpreeeion of human thrombopoietin increased the platelet level in the transfected mice. PMID: 11877062
    35. Mutations in the 5' untranslated region of the TPO gene are not the cause of the normal or elevated TPO levels in acquired essential thrombocythemia. PMID: 11860444
    36. REVIEW: central role in the pathogenesis of idiopathic thrombocytopenic purpura (ITP), andother immune-mediated thrombocytopenias. PMID: 11913997
    37. While there is increased platelet turnover in patients with chronic renal failure, the kidney does not seem to play a major role in the overall Tpo production in the body. PMID: 11960394
    38. binding to platelet thrombopoietin receptor is directly involved in human thrombopoietin plasma level regulation PMID: 11961237
    39. Flt3/Flk-2-ligand in synergy with thrombopoietin may slow down megakaryocyte development by causing increased proliferation of megakaryocyte progenitor cells. PMID: 11983110
    40. In the presence of EPO and SCF and/or IL-3, TPO enhances bone marrow erythropoiesis in cell cultures derived from patients with Diamond-Blackfan anemia. PMID: 12041668
    41. Thrombopoietin activates MAPKp42/44, AKT, and STAT proteins in normal human CD34+ cells, megakaryocytes, and platelets. PMID: 12135673
    42. The endogenous levels of TPO, IL-6 and IL-8 are elevated in the thrombocytopenic patients with AML and MDS. PMID: 12187073
    43. induces megakaryocyte-specific glycoprotein VI promoter and its regulation by GATA-1, Fli-1, and Sp1 PMID: 12359731
    44. no difference was found in the cord blood level of thrombopoietin between infants born to mothers with pregnancy-induced hypertension and those without PMID: 12381927
    45. review of TPO role in thrombopoiesis, signal transduction, cellular proliferative and anti-apoptotic mechanisms increasing megakaryocyte numbers PMID: 12430879
    46. levels in children with acute and chronic idiopathic thrombocytopenic purpura and its relationship with mega-dose methylprednisolone therapy PMID: 12468916
    47. Tpo concentrations in plasma samples taken concurrently from the right ventricle, the pulmonary artery, and the left ventricle showed there were positive correlations between the Tpo levels and pulmonary artery systolic pressure. PMID: 12487786
    48. TPO stimulation of a megakaryocyte cell line activated lyn kinase, shown to be involved in the transduction pathway of the TPO proliferative signal. PMID: 12495897
    49. Production of this protein in human hepatic cell cultures is not affected by IFN-alpha, IFN-beta, and IFN-gamma. PMID: 12581491
    50. c-mpl mutations are the cause not only for the hypomegakaryocytic thrombocytopenia, but also for the development of an aplastic anemia (AA) in patients with CAMT PMID: 12799278

    顯示更多

    收起更多

  • 相關(guān)疾病：
    Thrombocythemia 1 (THCYT1)
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    EPO/TPO family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 11795

    OMIM: 187950

    KEGG: hsa:7066

    STRING: 9606.ENSP00000204615

    UniGene: Hs.1166