ABCB1 Monoclonal Antibody
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中文名稱:ABCB1鼠單克隆抗體
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貨號(hào):CSB-MA001046A0m
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規(guī)格:¥1320
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圖片:
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Western Blot
Positive WB detected in: HepG2 whole cell lysate, 293 whole cell lysate, Hela whole cell lysate, SY5Y whole cell lysate, U251 whole cell lysate
All lanes: ABCB1 antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 141 kDa
Observed band size: 141-250 KDa
Exposure time:5min -
Immunofluorescence staining of HepG2 cells with CSB-MA001046A0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
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Immunofluorescence staining of Hela cells with CSB-MA001046A0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
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Overlay Peak curve showing Hela cells stained with CSB-MA001046A0m (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:
The ABCB1 monoclonal antibody is created by immunizing mice with recombinant human ABCB1 protein (350-710aa) and then fusing the mouse B cells with mouse myeloma cells to generate hybridoma cells. ABCB1 antibody-secreting hybridomas are selected to culture in the mouse abdominal cavity. The ABCB1 monoclonal antibody is purified and isolated from the mouse ascites using protein A affinity chromatography to achieve a purity of over 95%. It is an unconjugated IgG2b antibody that can specifically recognize human ABCB1 protein and is well-suited for use in ELISA, WB, IF, and FC applications.
ABCB1 protein, also known as P-glycoprotein (P-gp), is a member of the ATP-binding cassette (ABC) transporter family that plays a critical role in the efflux of a wide range of endogenous and xenobiotic compounds out of cells. It is highly expressed in various tissues including the intestine, liver, and kidney, where it acts as a primary gatekeeper to prevent the entry of harmful substances into the body or promote their removal from the body.
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產(chǎn)品名稱:Mouse anti-Homo sapiens (Human) ABCB1 Monoclonal antibody
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Uniprot No.:
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基因名:
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別名:ABC20 antibody; ABCB1 antibody; ATP binding cassette, sub family B (MDR/TAP), member 1 antibody; ATP-binding cassette sub-family B member 1 antibody; CD243 antibody; CLCS antibody; Colchicin sensitivity antibody; Doxorubicin resistance antibody; GP170 antibody; MDR1 antibody; MDR1_HUMAN antibody; Multidrug resistance 1 antibody; Multidrug resistance protein 1 antibody; P glycoprotein 1 antibody; P gp antibody; P-glycoprotein 1 antibody; PGY1 antibody
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宿主:Mouse
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反應(yīng)種屬:Human
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免疫原:Recombinant Human Calnexin protein (350-710AA)
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免疫原種屬:Homo sapiens (Human)
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標(biāo)記方式:Non-conjugated
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克隆類型:Monoclonal
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抗體亞型:IgG2b
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純化方式:>95%, Protein A purified
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克隆號(hào):5C12D3
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濃度:It differs from different batches. Please contact us to confirm it.
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保存緩沖液:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 -
產(chǎn)品提供形式:Liquid
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應(yīng)用范圍:ELISA, WB, IF, FC
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推薦稀釋比:
Application Recommended Dilution WB WB:1:1000-1:5000 IF 1:50-1:200 FC 1:50-1:200 -
Protocols:
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儲(chǔ)存條件:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期:Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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