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ABCB1 Monoclonal Antibody

  • 中文名稱：
    ABCB1鼠單克隆抗體
  • 貨號(hào)：
    CSB-MA001046A0m
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: HepG2 whole cell lysate, 293 whole cell lysate, Hela whole cell lysate, SY5Y whole cell lysate, U251 whole cell lysate
      All lanes: ABCB1 antibody at 1:1000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 141 kDa
      Observed band size: 141-250 KDa
      Exposure time：5min
    • Immunofluorescence staining of HepG2 cells with CSB-MA001046A0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
    • Immunofluorescence staining of Hela cells with CSB-MA001046A0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
    • Overlay Peak curve showing Hela cells stained with CSB-MA001046A0m (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    The ABCB1 monoclonal antibody is created by immunizing mice with recombinant human ABCB1 protein (350-710aa) and then fusing the mouse B cells with mouse myeloma cells to generate hybridoma cells. ABCB1 antibody-secreting hybridomas are selected to culture in the mouse abdominal cavity. The ABCB1 monoclonal antibody is purified and isolated from the mouse ascites using protein A affinity chromatography to achieve a purity of over 95%. It is an unconjugated IgG2b antibody that can specifically recognize human ABCB1 protein and is well-suited for use in ELISA, WB, IF, and FC applications.

    ABCB1 protein, also known as P-glycoprotein (P-gp), is a member of the ATP-binding cassette (ABC) transporter family that plays a critical role in the efflux of a wide range of endogenous and xenobiotic compounds out of cells. It is highly expressed in various tissues including the intestine, liver, and kidney, where it acts as a primary gatekeeper to prevent the entry of harmful substances into the body or promote their removal from the body.

  • 產(chǎn)品名稱：
    Mouse anti-Homo sapiens (Human) ABCB1 Monoclonal antibody
  • Uniprot No.：
  • 基因名：
  • 別名：
    ABC20 antibody; ABCB1 antibody; ATP binding cassette, sub family B (MDR/TAP), member 1 antibody; ATP-binding cassette sub-family B member 1 antibody; CD243 antibody; CLCS antibody; Colchicin sensitivity antibody; Doxorubicin resistance antibody; GP170 antibody; MDR1 antibody; MDR1_HUMAN antibody; Multidrug resistance 1 antibody; Multidrug resistance protein 1 antibody; P glycoprotein 1 antibody; P gp antibody; P-glycoprotein 1 antibody; PGY1 antibody
  • 宿主：
    Mouse
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    Recombinant Human Calnexin protein (350-710AA)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    IgG2b
  • 純化方式：
    >95%, Protein A purified
  • 克隆號(hào)：
    5C12D3
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IF, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB WB：1:1000-1:5000
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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