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Recombinant Human Claudin-9 (CLDN9)-VLPs (Active)

In Stock
  • 中文名稱：
  • 貨號(hào)：
    CSB-MP005511HU
  • 規(guī)格：
  • 圖片：
    • CSB-MP005511HU is detected by Mouse anti-6*His monoclonal antibody.
    • Activity
      Measured by its binding ability in a functional ELISA. Immobilized Human CLDN9 at 10 μg/ml can bind Anti-CLDN6/9 recombinant antibody (CSB-RA005508MA1HU), the EC50 is 24.93-35.47 ng/mL. Biological Activity Assay
  • 其他：

產(chǎn)品詳情

  • 內(nèi)毒素：
    Less than 1.0 EU/ug as determined by LAL method.
  • 生物活性：
    Measured by its binding ability in a functional ELISA. Immobilized Human CLDN9 at 10 μg/mL can bind Anti-CLDN6/9 recombinant antibody (CSB-RA005508MA1HU), the EC50 is 24.93-35.47 ng/mL.
  • 基因名：
  • Uniprot No.：
  • 分子結(jié)構(gòu)：
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長(zhǎng)度：
    Full Length
  • 來源：
    Mammalian cell
  • 分子量：
    24.2 kDa
  • 表達(dá)區(qū)域：
    1-217aa
  • 氨基酸序列
    MASTGLELLGMTLAVLGWLGTLVSCALPLWKVTAFIGNSIVVAQVVWEGLWMSCVVQSTGQMQCKVYDSLLALPQDLQAARALCVIALLLALLGLLVAITGAQCTTCVEDEGAKARIVLTAGVILLLAGILVLIPVCWTAHAIIQDFYNPLVAEALKRELGASLYLGWAAAALLMLGGGLLCCTCPPPQVERPRGPRLGYSIPSRSGASGLDKRDYV
  • 蛋白標(biāo)簽：
    C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產(chǎn)品描述：

    This Human Claudin-9 (CLDN9) recombinant protein was produced in Mammalian cell, where the gene sequence encoding Human CLDN9 (1-217aa) was expressed with the C-terminal 10xHis tag. The activity was validated.
    In addition, this Human CLDN9 recombinant protein was developed through the Virus-Like Particles (VLPs) Platform. It is a four-pass transmembrane protein.

    CLDN9 (Claudin-9) is located on human chromosome 16p13.3, with a full length of 651 bases. The CLDN9 gene is overexpressed in the blood, pituitary and brain, and is highly expressed in endometrial cancer, liver cancer and gastric cancer cell lines. CLDN9 protein is mainly Localized at cell tight junctions, cell membranes and channel proteins.
    CLDN9 is a tight junction protein. Abnormal expression of CLDN9 will lead to impaired tight junctions function and reduced barrier function, resulting in increased tissue permeability, which will eventually lead to many diseases including hereditary, allergic diseases, infectious diseases of various systems and even tumors. Therefore, CLDN9 is regarded as a potential target for the treatment of cancer or other diseases.

  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

平均分:
5.0分 - 1 個(gè)評(píng)價(jià)

實(shí)驗(yàn)應(yīng)用：藥物相關(guān)研究

產(chǎn)品評(píng)價(jià)： CLDN9被認(rèn)為是治療癌癥或其他疾病的潛在靶點(diǎn)。CLDN9是一個(gè)4次跨膜的蛋白，表達(dá)全長(zhǎng)難度很大。CUSA Bio通過其Vlp平臺(tái)表達(dá)的重組人CLDN9蛋白，具有完整的生物活性，我使用CSB-MP005511HU產(chǎn)品及抗CLDN9抗體(CSB-RA005508MA1HU)進(jìn)行ELISA實(shí)驗(yàn)，EC50為0.8032-2.566 ng/ml 。我司購(gòu)買了CUSABIO的很多VLP產(chǎn)品，都是常規(guī)很難表達(dá)的，我們測(cè)試活性都很好，對(duì)我司的藥物及研究貢獻(xiàn)很大。確實(shí)是很有實(shí)力的一家公司！尤其是VLP產(chǎn)品很有特色！

By 翟老師

靶點(diǎn)詳情

  • 功能：
    Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.; (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) entry into hepatic cells.
  • 基因功能參考文獻(xiàn)：
    1. CLDN9 may be an important biomarker for invasive pituitary oncocytomas. PMID: 25281028
    2. Claudins 6, 7, and 9 expressions are closely related to gastric carcinogenesis. PMID: 19960275
    3. Mouse studies suggest that claudin-9 deficiency may cause hearing loss not only in mice but also in humans. PMID: 19696885
    4. Residues N38 and V45 in the first extracellular loop (EL1) of CLDN9 are necessary for HCV entry. PMID: 17804490
    5. CLDN9, clustered with CLDN6 at human chromosome 16p13.3, is a four-transmembrane protein with WWCC motif, defined by W-X(17-22)-W-X(2)-C-X(8-10)-C. PMID: 12736707

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  • 亞細(xì)胞定位：
    Cell junction, tight junction. Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Claudin family
  • 組織特異性：
    Expressed in the liver, in peripheral blood mononuclear cells and hepatocarcinoma cell lines.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 2051

    OMIM: 615799

    KEGG: hsa:9080

    STRING: 9606.ENSP00000398017

    UniGene: Hs.296949