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Recombinant Human NKG2-D type II integral membrane protein (KLRK1), partial (Active)

In Stock
  • 中文名稱：
    人KLRK1重組蛋白
  • 貨號：
    CSB-MP012474HU1
  • 規(guī)格：
    ￥1368
  • 圖片：

    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

    • Activity
      Measured by its binding ability in a functional ELISA. Immobilized KLRK1 at 10 μg/ml can bind human ULBP1 (CSB-MP887177HU), the EC50 of human KLRK1 protein is 222.4-276.0 ng/ml.

      Biological Activity Assay

    • Human KLRK1 protein Fc tag (CSB-MP012474HU1) captured on COOH chip can bind Human ULBP1 protein Fc/myc tag (CSB-MP887177HU) with an affinity constant of 2.27 nM as detected by LSPR Assay. Biological Activity Assay

    • Activity
      Measured by its binding ability in a functional ELISA. Immobilized KLRK1 at 10 μg/ml can bind human Biotinylated ULBP1(CSB-MP887177HUj1-B), the EC50 is 4.254-7.295 ng/ml. Biological Activity Assay
    • The purity of KLRK1 was greater than 90% as determined by SEC-HPLC.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 90% as determined by SDS-PAGE.
    Greater than 90% as determined by SEC-HPLC.
  • 內(nèi)毒素：
    Less than 1.0 EU/ug as determined by LAL method.
  • 生物活性：
    ①Measured by its binding ability in a functional ELISA. Immobilized KLRK1 at 10 μg/ml can bind human ULBP1 (CSB-MP887177HU), the EC50 of human KLRK1 protein is 222.4-276.0 ng/ml.②Human KLRK1 protein Fc tag (CSB-MP012474HU1) captured on COOH chip can bind Human ULBP1 protein Fc/myc tag (CSB-MP887177HU) with an affinity constant of 2.27 nM as detected by LSPR Assay.③Measured by its binding ability in a functional ELISA. Immobilized KLRK1 at 10 μg/ml can bind human Biotinylated ULBP1(CSB-MP887177HUj1-B), the EC50 is 4.254-7.295 ng/ml.
  • 基因名：
  • Uniprot No.：
  • 別名：
    KLRK1; D12S2489E; NKG2D; NKG2-D type II integral membrane protein; Killer cell lectin-like receptor subfamily K member 1; NK cell receptor D; NKG2-D-activating NK receptor; CD antigen CD314
  • 分子結(jié)構(gòu)：
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Partial
  • 來源：
    Mammalian cell
  • 分子量：
    43.6 kDa
  • 表達區(qū)域：
    78-216aa
  • 氨基酸序列
    FLNSLFNQEVQIPLTESYCGPCPKNWICYKNNCYQFFDESKNWYESQASCMSQNASLLKVYSKEDQDLLKLVKSYHWMGLVHIPTNGSWQWEDGSILSPNLLTIIEMQKGDCALYASSFKGYIENCSTPNTYICMQRTV
  • 蛋白標簽：
    C-terminal hFc-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產(chǎn)品描述：

    The human NKG2-D type II integral membrane protein or KLRK1 is a costimulatory receptor expressed in Natural Killers and CD8+ T-cells. KLRK1 participates in the immunosurveillance of tumor and virus-infected cells and mediates the activation of T-cell receptors in CD8+ cells, causing the amplification of the T-cell-mediated adaptative immune response. This target protein KLRK1 also modulates natural immunity by activating the NK cell cytotoxic activity. It’s activated by the interaction of stress-induced ligands presented on the surface by the MHC class I binding proteins. This recombinant product is the 78-216aa region of the KLRK1 protein, which is expressed in mammalian cells and fused on the C-terminus with an Fc tag. It has a final molecular weight of 43.6 kDa. This product has a purity higher than 93%, as measure by SDS-PAGE. The affinity constant of this protein against ULBP1 was 2.27 nM as detected by LSPR, and the EC50 was 222.4-276.0 ng/ml, as determined by ELISA binding assay. The protein can be used in immunity modulation and immunosuppression studies on cancer and viral diseases. The final product has low levels of endotoxin, with less than 1.0 EU/µg as determined by the LAL method.

  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價

平均分:
5.0分 - 1 個評價

實驗應用：結(jié)合實驗/蛋白間互作

產(chǎn)品評價： 我們同時購買了2個產(chǎn)品，KLRK1產(chǎn)品和KLRK1 (CSB-MP012474HU1)進行ELISA實驗驗證，EC50為228.5-427.6 ng/ml，二者有較好的結(jié)合活性，蛋白純度也不錯，可重復性好，可以進一步用于后續(xù)蛋白互作研究實驗。

By 林老師

靶點詳情

  • 功能：
    Functions as an activating and costimulatory receptor involved in immunosurveillance upon binding to various cellular stress-inducible ligands displayed at the surface of autologous tumor cells and virus-infected cells. Provides both stimulatory and costimulatory innate immune responses on activated killer (NK) cells, leading to cytotoxic activity. Acts as a costimulatory receptor for T-cell receptor (TCR) in CD8(+) T-cell-mediated adaptive immune responses by amplifying T-cell activation. Stimulates perforin-mediated elimination of ligand-expressing tumor cells. Signaling involves calcium influx, culminating in the expression of TNF-alpha. Participates in NK cell-mediated bone marrow graft rejection. May play a regulatory role in differentiation and survival of NK cells. Binds to ligands belonging to various subfamilies of MHC class I-related glycoproteins including MICA, MICB, RAET1E, RAET1G, RAET1L/ULBP6, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4.
  • 亞細胞定位：
    Cell membrane; Single-pass type II membrane protein. Note=Colocalized with HCST on the cell surface.
  • 組織特異性：
    Expressed in natural killer (NK) cells, CD8(+) alpha-beta and gamma-delta T-cells. Expressed on essentially all CD56+CD3- NK cells from freshly isolated PBMC. Expressed in interferon-producing killer dendritic cells (IKDCs).
  • 數(shù)據(jù)庫鏈接：

    HGNC: 18788

    OMIM: 611817

    KEGG: hsa:100528032

    STRING: 9606.ENSP00000240618

    UniGene: Hs.387787