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Recombinant Human Nectin-4 (NECTIN4), partial (Active)

In Stock
  • 中文名稱：
    人NECTIN4重組蛋白
  • 貨號(hào)：
    CSB-MP822274HU
  • 規(guī)格：
    ￥1368
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Activity
      Measured by its binding ability in a functional ELISA. Immobilized NECTIN4 at 2 μg/ml can bind anti-NECTIN4 antibody（CSB-RA822274A0HU）（enfortumab vedotin-like）, the EC50 is 0.6029-0.7837 ng/mL. Biological Activity Assay
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 95% as determined by SDS-PAGE.
  • 內(nèi)毒素：
    Less than 1.0 EU/ug as determined by LAL method.
  • 生物活性：
    Measured by its binding ability in a functional ELISA. Immobilized NECTIN4 at 2 μg/mL can bind anti-NECTIN4 antibody（CSB-RA822274A0HU）（enfortumab?vedotin-like）, the EC50 is 0.6029-0.7837 ng/mL.
  • 基因名：
  • Uniprot No.：
  • 別名：
    (Ig superfamily receptor LNIR)(Nectin cell adhesion molecule 4)(Poliovirus receptor-related protein 4)
  • 分子結(jié)構(gòu)：
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長(zhǎng)度：
    Partial
  • 來(lái)源：
    Mammalian cell
  • 分子量：
    36.9 kDa
  • 表達(dá)區(qū)域：
    32-349aa
  • 氨基酸序列
    GELETSDVVTVVLGQDAKLPCFYRGDSGEQVGQVAWARVDAGEGAQELALLHSKYGLHVSPAYEGRVEQPPPPRNPLDGSVLLRNAVQADEGEYECRVSTFPAGSFQARLRLRVLVPPLPSLNPGPALEEGQGLTLAASCTAEGSPAPSVTWDTEVKGTTSSRSFKHSRSAAVTSEFHLVPSRSMNGQPLTCVVSHPGLLQDQRITHILHVSFLAEASVRGLEDQNLWHIGREGAMLKCLSEGQPPPSYNWTRLDGPLPSGVRVDGDTLGFPPLTTEHSGIYVCHVSNEFSSRDSQVTVDVLDPQEDSGKQVDLVSAS
  • 蛋白標(biāo)簽：
    C-terminal 10xHis-tagged
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產(chǎn)品描述：

    Recombinant human NECTIN4 protein is a mammalian cell-expressed partial length protein. Molecularly, this NECTIN4 protein is characterized by an N-terminal linker, internal human NECTIN4 DNA fragment (Gly32-Ser349), and a 10xHis tag at the C-terminus. Its purity is greater than 95% measured by SDS-PAGE. It has an apparent molecular weight of approximately 40 kDa on the gel. This slightly higher molecular mass results from glycosylation. This NECTIN4 protein contains low endotoxin, less than 1.0 EU/ug of the protein determined by the LAL method. Through a functional ELISA, this NECTIN4 protein has been validated to be active. It can bind to the anti-NECTIN4 antibody with an EC50 of 0.6029-0.7837 ng/mL.

    NECTIN4 participates in the intercellular connection and controls cell activities including cellular polarization, differentiation, motility, proliferation, and survival. Evidence has shown that NECTIN4 is overexpressed in multiple tumor cells but with a low expression in adult healthy tissues. NECTIN4, therefore, becomes a promising target for the diagnosis, prognosis, and treatment of many cancers. So far, a drug that combined the NECTIN4 antibody with MMAE has been developed and approved by FDA in December 2019 for the treatment of urothelial carcinoma.

  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

平均分:
5.0分 - 2 個(gè)評(píng)價(jià)

實(shí)驗(yàn)應(yīng)用：藥物相關(guān)研究

產(chǎn)品評(píng)價(jià)： NECTIN4在多種腫瘤細(xì)胞中過(guò)表達(dá)，但在成人健康組織中低表達(dá)，是我們研究的靶點(diǎn)之一。收到產(chǎn)品后，我們進(jìn)行SDS-PAGE檢測(cè)，條帶單一，無(wú)雜帶，蛋白純度高于標(biāo)準(zhǔn)！華美產(chǎn)品挺好用的。

By 周老師

實(shí)驗(yàn)應(yīng)用：藥物相關(guān)研究

產(chǎn)品評(píng)價(jià)： 這個(gè)蛋白與抗體進(jìn)行ELISA實(shí)驗(yàn)，EC50為0.7371-1.336ng/ml，顯示有較好的結(jié)合活性，后續(xù)用于抗體篩選，很順利！華美的蛋白做的很精細(xì)，技術(shù)質(zhì)控好，我們用量大，還會(huì)繼續(xù)大量采購(gòu)！

By 沈老師

靶點(diǎn)詳情

  • 功能：
    Seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.; (Microbial infection) Acts as a receptor for measles virus.
  • 基因功能參考文獻(xiàn)：
    1. We suggest that Nectin-4 is a relevant prognostic factor and a therapeutic target in luminalB (HER2 negative) breast cancer. PMID: 28778498
    2. Nectin-4 promotes cell-cell adhesion/aggregation, migration, and proliferation of ovarian tumor cells. PMID: 28038455
    3. Nectin-4 is not only a BCSC marker but also a breast cancer metastasis marker PMID: 28600142
    4. This study suggests that PVRL4 is post-transcriptionally regulated by miR-128 and miR-31. PMID: 27507538
    5. High Nectin-4 expression is associated with neoplasms. PMID: 27013195
    6. We show that Measles virus gains entry into MCF7, DLD-1, and HTB-20 cancer cells through a PVRL4-mediated macropinocytosis pathway and identified the typical cellular GTPase and kinase involved. PMID: 28250131
    7. nectin-4 serves as a stimulatory co-receptor for the prolactin receptor by regulating the feedback inhibition of SOCS1 in the JAK2-STAT5a signaling pathway PMID: 28258213
    8. Nectin-4 is both a new promising prognostic biomarker and specific therapeutic target for Triple-negative breast cancers PMID: 27998973
    9. ADAM17 and ADAM10 cleave Nectin-4 and release soluble Nectin-4 (sN4). PMID: 28232483
    10. The data presented in this study suggested that nectin-4 may be a therapeutic target for systemic lupus erythematous through affecting the cell apoptosis PMID: 26617807
    11. Nectin-4 is critical for gallbladder cancer cell progression via PI3K/AKT pathway activation of Rac1. PMID: 26949052
    12. Nectin-4 expression, when compared with control group, was higher in endometriotic lesions of patients having ovarian endometriosis and peritoneal endometriosis. This difference was significant in the endometrium of patients having endometriosis. PMID: 27328518
    13. Transformation of breast cancer cells is dependent on PVRL4. PMID: 23682311
    14. Nectin-4 is a significant prognostic predictor, and may play a critical role in pancreatic cancer. Nectin-4 may be novel therapeutic target for pancreatic cancer. PMID: 25888293
    15. In airway epithelial cells, measles virus spread requires the nectin-4/afadin complex and is based on cytoplasm transfer between columnar cells. PMID: 25926640
    16. The present study described clinical investigation of the EDSS1 identified in a large consanguineous family; DNA sequence analysis revealed a novel homozygous nonsense mutation (181C>T, p.Asp61*) in the PVRL4 gene. PMID: 25529316
    17. Ovarian tissue expression and serum nectin 4 appear to be potential markers in ovarian cancer. PMID: 25019423
    18. Authors show that the same key residues in the BC and FG loops of nectin-4 govern binding to the measles virus attachment protein hemagglutinin (H) and cell entry, nectin-4 homodimerization, and heterodimerization with nectin-1. PMID: 25275122
    19. data outline a synergistic action of nectin-1 and -4 in the early steps of AJ formation and implicate this interaction in modulating the Rac1 signaling pathway PMID: 24577405
    20. Ablation of nectin4 binding compromises CD46 usage by a hybrid vesicular stomatitis virus/measles virus. PMID: 24335299
    21. Thus, while nectin-4 and CD46 interact functionally with the measles virus H protein beta4-beta5 hydrophobic groove, SLAM merely covers it. PMID: 23760251
    22. Data suggest that expression of nectin-4 is up-regulated in eutopic endometrium of patients with endometriosis compared with control subjects. PMID: 22926846
    23. We show that human nectin4 is fully functional as a canince distemper virus receptor. PMID: 23174504
    24. structure of the membrane-distal domain of nectin-4 in complex with measles virus hemagglutinin (MV-H; structure shows nectin-4 binds the MV-H beta4-beta5 groove exclusively via its N-terminal IgV domain; the contact interface is dominated by hydrophobic interactions PMID: 23202587
    25. crystals of nectin-4 diffracted to 1.8 A resolution and belonged to space group P2(1), with unit-cell parameters a = 33.1, b = 51.7, c = 56.9 A, beta = 94.7 degrees PMID: 22869128
    26. measles virus targets nectin-4 to emerge in the airways PMID: 22048310
    27. Measles virus infection reduced PVRL4 surface expression in MCF7 cells, a property that is characteristic of receptor-associated viral infections. PMID: 21901103
    28. The findings of study suggest that increased expression of Nectin-4 may indicate a worse prognosis in breast cancer patients. PMID: 21526486
    29. Sequence analysis revealed a homozygous missense mutation (c.635C>G; p.Pro212Arg) in the recently reported PVRL4 gene causing EDSS1 PMID: 21346770
    30. Ectoderma dysplasia-syndactyly syndrome. Second example of "nectinopathy". PMID: 21333831
    31. nectin 4 may serve as a potential biomarker that helps discriminate benign gynecologic diseases from ovarian cancer in a panel with CA125. PMID: 20959669
    32. Ectodermal dysplasia-syndactyly syndrome is the second known "nectinopathy" caused by mutations in a nectin adhesion molecule. PMID: 20691405
    33. the active form of TACE is overexpressed in breast tumors and may indicate that TACE is responsible for Nectin-4 shedding not only in vitro but also in vivo PMID: 15784625
    34. Nectin-4 was not detected in normal breast epithelium; by contrast Nectin-4 was expressed in ductal breast carcinoma. PMID: 17474988
    35. Nectin-4 might play a significant role in lung carcinogenesis, and it should be a new candidate serum and tissue biomarker, as well as a therapeutic target. PMID: 19679554

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  • 相關(guān)疾?。?/div>
    Ectodermal dysplasia-syndactyly syndrome 1 (EDSS1)
  • 亞細(xì)胞定位：
    Cell membrane; Single-pass type I membrane protein. Cell junction, adherens junction. Note=Colocalizes with AFDN at cadherin-based adherens junctions (PubMed:11544254).; [Processed poliovirus receptor-related protein 4]: Secreted. Note=The secreted form is found in breast tumor patients (PubMed:15784625).
  • 蛋白家族：
    Nectin family
  • 組織特異性：
    Predominantly expressed in placenta. Not detected in normal breast epithelium but expressed in breast carcinoma.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 19688

    OMIM: 609607

    KEGG: hsa:81607

    STRING: 9606.ENSP00000356991

    UniGene: Hs.492490