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Recombinant Mouse Claudin-18 (Cldn18)-VLPs (Active)

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  • 中文名稱(chēng)：
  • 貨號(hào)：
    CSB-MP005498MO(F3)
  • 規(guī)格：
  • 圖片：
    • CSB-MP005498MO(F3) is detected by Mouse anti-6*His monoclonal antibody.
    • Activity
      Measured by its binding ability in a functional ELISA. Immobilized Mouse Cldn18 at 5 μg/ml can bind Anti-CLDN18.2 recombinant antibody (CSB-RA005498A1HU), the EC50 is 6.115-11.01 ng/mL. Biological Activity Assay
  • 其他：

產(chǎn)品詳情

  • 內(nèi)毒素：
    Less than 1.0 EU/ug as determined by LAL method.
  • 生物活性：
    Measured by its binding ability in a functional ELISA. Immobilized Mouse Cldn18 at 5 μg/mL can bind Anti-CLDN18.2 recombinant antibody (CSB-RA005498A1HU), the EC50 is 6.115-11.01 ng/mL.
  • 基因名：
    Cldn18
  • Uniprot No.：
  • 分子結(jié)構(gòu)：
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長(zhǎng)度：
    Full Length of Isoform A2.1
  • 來(lái)源：
    Mammalian cell
  • 分子量：
    29.1 kDa
  • 表達(dá)區(qū)域：
    1-264aa
  • 氨基酸序列
    MSVTACQGLGFVVSLIGFAGIIAATCMDQWSTQDLYNNPVTAVFNYQGLWRSCVRESSGFTECRGYFTLLGLPAMLQAVRALMIVGIVLGVIGILVSIFALKCIRIGSMDDSAKAKMTLTSGILFIISGICAIIGVSVFANMLVTNFWMSTANMYSGMGGMGGMVQTVQTRYTFGAALFVGWVAGGLTLIGGVMMCIACRGLTPDDSNFKAVSYHASGQNVAYRPGGFKASTGFGSNTRNKKIYDGGARTEDDEQSHPTKYDYV
  • 蛋白標(biāo)簽：
    N-terminal 6xHis-tagged (This tag can be tested only under denaturing conditions)
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產(chǎn)品描述：

    This Mouse Claudin-18 (Cldn18) recombinant protein was produced in mammalian cell, where the gene sequence encoding mouse Cldn18 (1-264aa) was expressed with the N-terminal 6xHis tag. The activity was validated. In addition, this mouse Cldn18 recombinant protein was developed through the Virus-Like Particles (VLPs) Platform. It is a four-pass transmembrane protein.

    CLDN18 belongs to one of the tight junction protein family, and its isoform CLDN18.2 is highly expressed in a variety of tumor tissues, such as gastric cancer (60%-80%), pancreatic cancer (50%), esophageal cancer (30%-50%), and lung cancer (40%~60%), etc., but almost no expression in normal tissues, so it has excellent therapeutic potential. Even in the metastatic lesions of gastric cancer, CLDN18.2 has a high expression level, which makes CLDN18.2 a potential target for targeted therapy and immunotherapy.
    CLDN18 was initially identified as a target gene of the transcription factor t/ebp/nkx2.1, consistent with its homology to other Claudin family members, and CLDN18 was later shown to localize at cellular tight junctions in mice and humans. The full-length murine CLDN18 protein is 264 amino acids, and its homology with human CLDN18.2 protein is as high as 90%. Its structure is similar to that of human. Both of them have four transmembrane domains: the intracellular NH2 terminus & COOH-terminus and two extracellular loops ECL1 & ECL2.

     

  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
  • 基因功能參考文獻(xiàn)：
    1. The presented data substantiate the hypothesis that claudin-18 is a central barrier-forming component of tight junctions and show that IL-13 downregulates claudin-18. These data also suggest that the loss of claudin-18 is associated with increased sensitization to aeroantigens and airway responsiveness PMID: 27215490
    2. The claudin-18 knockout mice exhibited decreased P2X7 mRNA transcript abundance as measured by mRNA expression microarray PMID: 27663455
    3. CLDN18 deficiency results in epithelial barrier dysfunction, injury, and impaired alveolarization in mice. PMID: 24787463
    4. Identify a role for claudin 18 in alveolar fluid homeostasis beyond its direct contributions to barrier properties. PMID: 24588076
    5. we conclude that the estrogen effects on osteoclasts may in part be mediated via regulation of Cldn-18 signaling PMID: 23299504
    6. Claudin-18 is expressed at the variety of epithelial tissues in inner ear including Organ of Corti, stria vascularis, Reissner's membrane, spiral limbus, vestibular sensory epithelia, and dark cell area. PMID: 14698084
    7. Cldn-18 is a novel negative regulator of bone resorption and osteoclast differentiation PMID: 22437732
    8. Claudin-18 forms a paracellular barrier against H(+) in the stomach. Deficiency causes paracellular H(+) leak, up-regulation of proinflammatory cytokines, recruitment of neutrophils, and atrophic gastritis. PMID: 22079592
    9. Increased expression of claudin-18 is associated with ulcerative colitis. PMID: 18831034

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  • 亞細(xì)胞定位：
    Cell junction, tight junction. Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Claudin family
  • 組織特異性：
    Isoform A1.1: Expressed in lung. Isoform A1.2: Expressed in lung. Isoform A2.1: Expressed in stomach. Isoform A2.2: Expressed in stomach.
  • 數(shù)據(jù)庫(kù)鏈接：