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BDH1 Antibody

  • 中文名稱：
    BDH1兔多克隆抗體
  • 貨號：
    CSB-PA002648LA01HU
  • 規(guī)格：
    ￥3,900
  • 促銷：
    小規(guī)格抗體限時一口價
  • 圖片：
    • Western Blot
      Positive WB detected in:Mouse Heart tissue lysate, Mouse Liver tissue lysate, Mouse Brain tissue lysate, Rat Heart tissue lysate, Rat Livert tissue lysate, Rat Brain tissue lysate, MCF7 whole cell lysate
      All lanes: BDH1 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 39 kDa
      Observed band size: 31 kDa
    • IHC image of CSB-PA002648LA01HU diluted at 1:150 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
    • IHC image of CSB-PA002648LA01HU diluted at 1:150 and staining in paraffin-embedded mouse liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
    • Immunofluorescence staining of MCF-7 cell with CSB-PA002648LA01HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence staining of MCF-7 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence staining of U251 cell with CSB-PA002648LA01HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence staining of U251 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    The BDH1 polyclonal antibody is a type of antibody generated in rabbits using recombinant human D-beta-hydroxybutyrate dehydrogenase, mitochondrial protein (47-343aa) as an immunogen. This antibody has been purified using Protein G, resulting in a purity level greater than 95%. This ensures that the antibody is of high quality and free from impurities that may interfere with experimental results.

    The BDH1 polyclonal antibody can recognize BDH1 protein in both human and mouse samples. The BDH1 polyclonal antibody has been tested in a range of applications, including ELISA, WB, and IHC, for the quantitative or qualitative detection, determination of the presence and size, detection of the localization and expression levels of BDH1 protein.

  • 產(chǎn)品名稱：
    Rabbit anti-Homo sapiens (Human) BDH1 Polyclonal antibody
  • Uniprot No.：
  • 基因名：
    BDH1
  • 別名：
    3 hydroxybutyrate dehydrogenase (heart; mitochondrial) antibody; 3-hydroxybutyrate dehydrogenase antibody; BDH antibody; BDH_HUMAN antibody; BDH1 antibody; D beta hydroxybutyrate dehydrogenase; mitochondrial antibody; D-beta-hydroxybutyrate dehydrogenase antibody; mitochondrial antibody
  • 宿主：
    Rabbit
  • 反應種屬：
    Human, Mouse
  • 免疫原：
    Recombinant Human D-beta-hydroxybutyrate dehydrogenase, mitochondrial protein (47-343AA)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated

    本頁面中的產(chǎn)品，BDH1 Antibody (CSB-PA002648LA01HU)，的標記方式是Non-conjugated。對于BDH1 Antibody，我們還提供其他標記。見下表：

    可提供標記
    標記方式 貨號 產(chǎn)品名稱 應用
    HRP CSB-PA002648LB01HU BDH1 Antibody, HRP conjugated ELISA
    FITC CSB-PA002648LC01HU BDH1 Antibody, FITC conjugated
    Biotin CSB-PA002648LD01HU BDH1 Antibody, Biotin conjugated ELISA
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    Antigen?affinity?purification
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.02% sodium azide
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC, IF
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:3000
    IHC 1:100-1:300
    IF 1:20-1:100
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

產(chǎn)品評價

靶點詳情

  • 基因功能參考文獻：
    1. Data show that the ketone body metabolizing enzymes BDH1, BDH2, OXCT1 and ACAT1 were expressed at the mRNA and protein level in all glioma cell lines. PMID: 21791085
  • 亞細胞定位：
    Mitochondrion inner membrane. Mitochondrion matrix.
  • 蛋白家族：
    Short-chain dehydrogenases/reductases (SDR) family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 1027

    OMIM: 603063

    KEGG: hsa:622

    STRING: 9606.ENSP00000350914

    UniGene: Hs.274539