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CDK5RAP2 Antibody

  • 中文名稱：
    CDK5RAP2兔多克隆抗體
  • 貨號：
    CSB-PA030230
  • 規(guī)格：
    ￥880
  • 其他：

產(chǎn)品詳情

  • Uniprot No.：
  • 基因名：
  • 別名：
    C48 antibody; CDK5 activator-binding protein C48 antibody; CDK5 regulatory subunit associated protein 2 antibody; CDK5 regulatory subunit-associated protein 2 antibody; CDK5RAP2 antibody; centrosomal protein 215 kDa antibody; Centrosome-associated protein 215 antibody; CEP215 antibody; CK5P2_HUMAN antibody; MCPH3 antibody
  • 宿主：
    Rabbit
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    Synthesized peptide derived from the N-terminal region of Human CDK5 Activator-binding C48.
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 抗體亞型：
    IgG
  • 純化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    IHC, ELISA
  • 推薦稀釋比：
    Application Recommended Dilution
    IHC 1:100-1:300
    ELISA 1:20000
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

產(chǎn)品評價

靶點詳情

  • 功能：
    Potential regulator of CDK5 activity via its interaction with CDK5R1. Negative regulator of centriole disengagement (licensing) which maintains centriole engagement and cohesion. Involved in regulation of mitotic spindle orientation. Plays a role in the spindle checkpoint activation by acting as a transcriptional regulator of both BUBR1 and MAD2 promoter. Together with EB1/MAPRE1, may promote microtubule polymerization, bundle formation, growth and dynamics at the plus ends. Regulates centrosomal maturation by recruitment of the gamma-tubulin ring complex (gamma-TuRC) onto centrosomes. In complex with PDE4DIP isoform 13/MMG8/SMYLE, MAPRE1 and AKAP9, contributes to microtubules nucleation and extension from the centrosome to the cell periphery. Required for the recruitment of AKAP9 to centrosomes. Plays a role in neurogenesis.
  • 基因功能參考文獻(xiàn)：
    1. ASPM protein is a spindle pole-focusing factor that functions redundantly with CDK5RAP2. PMID: 28883092
    2. A frameshift mutation in CDK5RAP2 gene is associated with primary microcephaly with speech impairment and sparse eyebrows in a consanguineous Pakistani family. PMID: 28778786
    3. Using homozygosity mapping complemented with whole-exome, gene panel or Sanger sequencing, we identified 12 novel mutations in 3 known MCPH-associated genes - 9 in ASPM, 2 in MCPH1 and 1 in CDK5RAP2. The 2 MCPH1 mutations were homozygous microdeletions of 164,250 and 577,594 bp, respectively, for which we were able to map the exact breakpoints PMID: 28004384
    4. Data suggest that CDK5RAP2 and CEP170 both interact with microtubule nucleation-promoting region of AKAP350A; CEP68 interacts with distal C-terminal region of AKAP350A; AKAP350A spans the bridge between centrioles. (CDK5RAP2 = CDK5 regulatory subunit associated protein 2; CEP170 = centrosomal protein 170kDa; AKAP350A = A kinase (PRKA) anchor protein (yotiao) 9; CEP68 = centrosomal protein 68kDa) PMID: 29054927
    5. results suggest that EB1 cooperates with CDK5RAP2 and perhaps other SXIP-containing +TIPs in tracking growing microtubule tips. PMID: 28320860
    6. CDK5RAP2 may play a role in primary microcephaly and interacts with components of the Hippo signaling pathway PMID: 28004182
    7. Three siblings with isolated agenesis of corpus callosum carry compound heterozygous variants, p.[Gly94Arg];[Asn1232Ser], in CDK5RAP2 gene. PMID: 26197979
    8. stabilization of the centrosome-spindle pole interface by the CEP215-HSET complex could promote survival of cancer cells containing supernumerary centrosomes. PMID: 26987684
    9. mouse expresses only one form of CDK5RAP2 that is equivalent to the human and rat alternatively spliced variant forms PMID: 26550838
    10. These results show that Cep169 targets microtubule tips and regulates stability of microtubules with CDK5RAP2. PMID: 26485573
    11. Cep68 degradation allows Cep215 removal from peripheral pericentriolar material (PCM) preventing centriole separation following disengagement, PCNT cleavage mediates Cep215 removal from core of the PCM to inhibit centriole disengagement and duplication PMID: 25503564
    12. LRRK1 regulates mitotic spindle orientation downstream of PLK1 through CDK5RAP2-dependent centrosome maturation. PMID: 26192437
    13. The CEP215-pericentrin interaction is required for centrosome maturation and subsequent bipolar spindle formation during mitosis. PMID: 24466316
    14. DPP4, CDK5RAP2, and CCR6 are risk loci for rheumatoid arthritis in Han Chinese and congruence with risk variants in Europeans. PMID: 24782177
    15. results reveal a key role of the dynein-dynactin complex in the dynamic recruitment of CDK5RAP2 to centrosomes PMID: 23874654
    16. Human expression pattern of CDK5RAP2 is similar to that seen in mice and is in concordance with pathology suggested by neuroimaging studies in humans and mouse PMID: 22806269
    17. Sequencing of the coding exons and exon/intron splice junctions of the CDK5RAP2 gene identified homozygosity for the novel nonsense mutation, c.4441C > T (p.Arg1481*), in both affected sons PMID: 23587236
    18. We conclude that the common variations we measured in the 4 microcephaly genes, ASPM, MCPH1, CDK5RAP2, and CENPJ, do not affect the risk of Alzheimer disease PMID: 21297427
    19. The deletion of the C-terminal structural maintenance of chromosome (SMC) domain and the p35-binding domain in both patient pedigrees 1 and 2 CDK5RAP2 is sufficient for the development of MCPH3-associated primary microcephaly. PMID: 21512315
    20. A report of an novel alternatively spliced variant form of hCDK5RAP2, hCDK5RAP2 variant 1 (hCDK5RAP2-V1) which lacks the 237 nucleotide residues of hCDK5RAP2 exon 32. PMID: 21346412
    21. CDK5RAP2 may represent a novel mechanism for centrosomal and Golgi localization. PMID: 20466722
    22. CEP 215 regulates a dynein-dependent transport of the pericentriolar matrix proteins during the centrosome maturation. PMID: 20139723
    23. Mutations in CDK5RAP2 gene is associated with autosomal recessive primary microcephaly PMID: 15793586
    24. pattern of molecular evolution seen in CDK5RAP2 appears to parallel, at least approximately, that seen in two other previously identified primary microcephaly genes, microcephalin and ASPM PMID: 16631324
    25. Centrosome malfunction due to the CDK5RAP2 mutations may underlie autosomal recessive primary microcephaly. PMID: 17959831
    26. Cep215 associates with centrosomes throughout the cell cycle and does not appear to interact with Cep68, rootletin or C-Nap1; data suggest Cep215 functionally interacts with pericentrin PMID: 18042621
    27. CDK5RAP2 is required for spindle checkpoint function. PMID: 19282672
    28. Results show that CDK5RAP2 targets growing microtubule tips in association with EB1 to regulate microtubule dynamics. PMID: 19553473

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  • 相關(guān)疾?。?/div>
    Microcephaly 3, primary, autosomal recessive (MCPH3)
  • 亞細(xì)胞定位：
    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Golgi apparatus. Cytoplasm. Cytoplasm, cytoskeleton.
  • 組織特異性：
    Widely expressed. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 18672

    OMIM: 604804

    KEGG: hsa:55755

    STRING: 9606.ENSP00000343818

    UniGene: Hs.269560