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CLK1 Antibody

  • 中文名稱：
    CLK1兔多克隆抗體
  • 貨號：
    CSB-PA005557ESR2HU
  • 規(guī)格：
    ￥440
  • 促銷：
    小規(guī)格抗體限時一口價
  • 圖片：
    • Western blot
      All lanes: CLK1antibody at 3.07μg/ml
      Lane 1: HepG2 whole cell lysate
      Lane 2: Jurkat whole cell lysate
      Lane 3: U251 whole cell lysate
      Lane 4: A549 whole cell lysate
      Secondary
      Goat polyclonal to rabbit IgG at 1/10000 dilution
      Predicted band size: 58, 17, 62 kDa
      Observed band size: 58 kDa
    • Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using CSB-PA005557ESR2HU at dilution of 1:100
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品名稱：
    Rabbit anti-Homo sapiens (Human) CLK1 Polyclonal antibody
  • Uniprot No.：
  • 基因名：
    CLK1
  • 別名：
    CDC like kinase 1 antibody; CDC-like kinase 1 antibody; CDC28/CDC2 like kinase antibody; CLK antibody; CLK/STY antibody; Clk1 antibody; CLK1_HUMAN antibody; Dual specificity protein kinase CLK1 antibody; Protein tyrosine kinase STY antibody; STY antibody
  • 宿主：
    Rabbit
  • 反應種屬：
    Human
  • 免疫原：
    Recombinant Human Dual specificity protein kinase CLK1 protein (1-130AA)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    Antigen Affinity Purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • 產(chǎn)品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    IHC 1:20-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

產(chǎn)品評價

靶點詳情

  • 功能：
    Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex and may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates: SRSF1, SRSF3 and PTPN1. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells and adenovirus E1A pre-mRNA.
  • 基因功能參考文獻：
    1. We now show that the ability of SRPK1 to mobilize SRSF1 from speckles to the nucleoplasm is dependent on active CLK1. Diffusion from speckles is promoted by the formation of an SRPK1-CLK1 complex that facilitates dissociation of SRSF1 from CLK1 and enhances the phosphorylation of several serine-proline dipeptides in this SR protein PMID: 29335301
    2. Data suggest that CLK1 activity in both transformed and normal cells is carefully regulated via CLK1 alternative splicing through both exon 4 skipping and intron 4 retention. CLK1 autoregulates by favoring the expression of truncated isoforms, CLK1T1 and CLKT2 in environmental stress. PMID: 29802995
    3. These results thus reveal a large program of CLK1-regulated periodic alternative splicing intimately associated with cell cycle control. PMID: 27015110
    4. Global CLK-dependent exon recognition and conjoined gene formation revealed with a novel small molecule inhibitor has been described. PMID: 28232751
    5. that mitochondrial Clk1 regulated chemoresistance in glioma cells through AMPK/mTOR/HIF-1alpha mediated glycolysis pathway PMID: 28581641
    6. SRPK1 interacts with an RS-like domain in the N terminus of CLK1 to facilitate the release of phosphorylated SR proteins, which then promotes efficient splice-site recognition and subsequent spliceosome assembly. PMID: 27397683
    7. Clk1, Clk2 and Clk4 prevent chromatin breakage by regulating the Aurora B-dependent abscission checkpoint. PMID: 27126587
    8. removal of the N-terminus or dilution of CLK1 induces monomer formation and reverses this specificity. CLK1 self-association also occurs in the nucleus PMID: 26443864
    9. Nuclear CLK-1 mediates a retrograde signalling pathway that is conserved from Caenorhabditis elegans to humans and is responsive to mitochondrial reactive oxygen species, thus acting as a barometer of oxidative metabolism. PMID: 25961505
    10. Data suggest that proline phosphorylation by CLK1/CDC-like kinase 1 (but not by SRPK1/serine/arginine-rich splicing factor kinase 1) regulates conformation and alternative splicing function of SFRS1 (serine/arginine-rich splicing factor 1). PMID: 25529026
    11. findings suggest that CLK1-dependent hyperphosphorylation is the result of a general mechanism in which the N-terminus acts as a bridge connecting the kinase domain and the RS domain of the SR protein. PMID: 24869919
    12. The data establish a new view of SRSF1 protein regulation in which SRPK1 and CLK1 partition activities based on Ser-Pro versus Arg-Ser placement rather than on N- and C-terminal preferences along the RS domain. PMID: 23707382
    13. CLK1 Increases While CLK2 Decreases HIV-1 Gene Expression. PMID: 21682887
    14. Clk/Sty is found in the nucleus of several different cell types and is a factor directly involved in splicing control PMID: 12773558
    15. ASF/SF2 is phosphorylated by SRPK1 and Clk/Sty PMID: 16223727
    16. Cdc2-like kinases and DNA topoisomerase I regulate alternative splicing of tissue factor in human endothelial cells. PMID: 19168442

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  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    Protein kinase superfamily, CMGC Ser/Thr protein kinase family, Lammer subfamily
  • 組織特異性：
    Endothelial cells.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 2068

    OMIM: 601951

    KEGG: hsa:1195

    STRING: 9606.ENSP00000394734

    UniGene: Hs.433732