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DNMT3B Antibody

  • 中文名稱：
    DNMT3B兔多克隆抗體
  • 貨號(hào)：
    CSB-PA002178
  • 規(guī)格：
    ￥880
  • 圖片：
    • Western Blot analysis of HT29 cells using Dnmt3b Polyclonal Antibody
  • 其他：

產(chǎn)品詳情

  • Uniprot No.：
  • 基因名：
    DNMT3B
  • 別名：
    Cytosine 5methyltransferase 3B antibody; DNA antibody; DNA (cytosine 5) methyltransferase 3 beta antibody; DNA (cytosine 5)-methyltransferase 3B antibody; DNA (cytosine-5)-methyltransferase 3B antibody; DNA methyltransferase HsaIIIB antibody; DNA MTase HsaIIIB antibody; DNM3B_HUMAN antibody; Dnmt3b antibody; EC 2.1.1.37 antibody; ICF antibody; ICF1 antibody; M.HsaIIIB antibody; MGC124407 antibody; RP23-89H14.3 antibody
  • 宿主：
    Rabbit
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    Synthesized peptide derived from the N-terminal region of Human Dnmt3b.
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 抗體亞型：
    IgG
  • 純化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    WB, IHC, IF, ELISA
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:100-1:300
    IF 1:200-1:1000
    ELISA 1:20000
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing. In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs. Functions as a transcriptional corepressor by associating with ZHX1. Required for DUX4 silencing in somatic cells.
  • 基因功能參考文獻(xiàn)：
    1. The following meta-analysis involved only Chinese population and further confirmed the significant association of -579G>T with gastric cancer but not lung cancer and suggested no significant association between -149C>T and risk of lung or gastric cancer. Collectively, DNMT3B -579G>T polymorphism is associated with gastric cancer risk in Chinese population PMID: 29332452
    2. Among 9 candidate genes, GNB4 was identified and validated by qRT-PCR as a potential target silenced by DNA methylation via DNA methyltransferase 3B (DNMT3B). PMID: 30103729
    3. Study suggests a role for DNMT3B in leukemogenesis in inv(16) acute myeloid leukemia, through MN1 methylation regulation. PMID: 28892045
    4. Data indicate a role for DNA methyltransferase 3B (DNMT3B) suggesting that it might substitute for the absent accessory protein DNMT3L to recruit DNMT3A in somatic cells. PMID: 27121154
    5. These results provided evidence that the DNMT3B -283T>C polymorphism might significantly contribute to the lung cancer risk in the Asian population, but not the gastric cancer risk in the Chinese population. PMID: 29027179
    6. There was no significant difference in the distribution of DNMT3B -579 G>T genotypes between the cases and controls. PMID: 28220295
    7. The epigenetic targets AURKB, AURKC and DNMT3B, and the global DNA methylation profile are regulated during HIV-1 replication in CD4+ T cells, and this regulation can be influenced by the activation state of the cell at the time of infection. PMID: 30077875
    8. Data suggest that female patients carrying the rs2424932 or rs998382 variants of DNA methyltransferase 3b (DNMT3) were more likely to develop Parkinson's disease (PD) than female controls. PMID: 28990350
    9. Our study expands the mutation spectrum in ICF syndrome and supports that DNMT3B and ZBTB24 are the most common disease genes PMID: 28128455
    10. Alternative splice variant of DNMT3B is associated with leukemia. PMID: 28730333
    11. these results provided a plausible link between the observed reduction of miR-26a and MEG3 in HCCs. Together, the present study added miR-26a/DNMT3B/MEG3 axis to the complex mechanisms of HCC development. PMID: 28440439
    12. Results show that DNMT1 mRNA levels were significantly higher in alveolar rhabdomyosarcoma and embryonal rhabdomyosarcoma tumors compared to normal skeletal muscle. Thse data indicate that altered expression of DNMT3B plays a key role in embryonal rhabdomyosarcoma development since its silencing is able to reverse cell cancer phenotype by rescuing myogenic program. PMID: 27764816
    13. The present study shows that DNMT3B rs2424913 promotor polymorphism represents a genetic risk factor that may play an important role in understanding the pathogenesis of chronic immune thrombocytopenia. PMID: 27590349
    14. LMP1-mediated NF-kappaB can up-regulate DNMT3b transcription, thereby leading to relatively higher methylation intensity at PTEN CpG islands, and ultimately silencing major tumor suppressor PTEN PMID: 27223069
    15. report the first crystal structure of the DNMT3B PWWP domain-H3K36me3 complex. PMID: 26993463
    16. Results continue to establish ANG as an oncoprotein and further reveal that ANG contributes to oncogenesis by the activation of MMP2 through modulation of DNMT3b functions. PMID: 27317771
    17. found association between DNMT3B rs2424913 in T allele carriers with Parkinson's disease PMID: 28041964
    18. H19 might function as ceRNA (competing endogenous RNA) for miR-29b-3p and relieve the suppression for DNMT3B, which led to EMT and metastasis of bladder cancer (BC). Our findings highlight a novel mechanism of H19 in progression of BC and provide H19/miR-29b-3p/DNMT3B axis as a promising therapeutic target for BC. PMID: 28779971
    19. A novel homozygous missense mutation, Ala585Thr, was found in DNMT3B. PMID: 27734333
    20. Definitive diagnosis should be done using metaphase analysis to identify centromeric instability and/or ICF disease gene mutations analysis. Bilateral VUR may occur in ICF patients with homozygous DNMT3B mutations in early childhood. Renal ultrasonography should be included in ICF1 patients for the screening of congenital anomalies. PMID: 27604394
    21. DNMT 3B was upregulated in invasive subclones and exerted on influence of E-cad gene methylation. PMID: 27391030
    22. Transduction of miR-339 and miR-766 expressing viruses into colon cancer cell lines (SW480 and HCT116) decreased DNMT3B expression (1.5, 3-fold) and (3, 4-fold), respectively. In addition, DNA methylation of some tumor suppressor genes decreased. PMID: 27668319
    23. Immunodeficiency, centromere instability and facial anomalies syndrome specific DNMT3B dysfunction interferes with intragenic regulation of mRNA transcription and alternative splicing. PMID: 28334849
    24. nickel exposure results in DNMT3b induction and MEG3 promoter hypermethylation and expression inhibition, further reduces its binding to c-Jun and in turn increasing c-Jun inhibition of PHLPP1 transcription, leading to the Akt/p70S6K/S6 axis activation, and HIF-1alpha protein translation, as well as malignant transformation of human bronchial epithelial cells. PMID: 28263966
    25. Overexpression of MAEL in UCB cells substantially enhanced the enrichment of DNA methyltrans-ferase (DNMT)3B and histone deacetylase (HDAC)1/2 on the promoter of the MTSS1, and thereby epigenetically suppressing the MTSS1 transcription. PMID: 27181205
    26. Data suggest that the overexpression of DNMT3B4 may play an important role in human kidney tumorigenesis through chromosomal instability and methylation of RASSF1A. PMID: 28160561
    27. Dnmt3b plays a tumor suppressive role in MLL-AF9 AML progression PMID: 27133822
    28. data indicated that Kaempferol is a novel DNMT3B inhibitor, which may promote the degradation of DNMT3B in bladder cancer. PMID: 28278502
    29. Findings suggest that PTEN and DNMT3B possess common regulation features as well as certain ethnic differences in expression between Han women and Uygur women. PMID: 28220037
    30. This condensed chromatin structure is associated with binding of DNMT3B and decreased occupancy of OCT1 transcription factor at MAML2 enhancer, suggesting a role of DNMT3B in increasing methylation of MAML2 after stilbenoid treatment. PMID: 27207652
    31. show that heterozygous mutations in DNA methyltransferase 3B (DNMT3B) are a likely cause of D4Z4 derepression associated with low levels of DUX4 expression PMID: 27153398
    32. 149C>T and -2437T>A DNMT3B polymorphisms are not correlated with lung cancer risk among Chinese population nor the haplotype of them. PMID: 27876061
    33. mRNA and protein expression levels of DNMT3b were upregulated in genotype 1b and 3a HCV-infected hepatocellular carcinoma patients as compared to control. DNMT3b mRNA levels did not change in genotypes 2a, 3, and 4, but were upregulated at the protein level by genotype 1b, 2a, and 3a. No differences were seen for genotypes 5 and 7. PMID: 26850594
    34. Haplotype analysis reveals a strong association of the interaction of DNMT3B gene with APOEepsilon4 in a sample of Alzheimer disease patients. PMID: 27188425
    35. DNMT3B Gene Polymorphism is associated with Gastric and Colorectal Cancer. PMID: 27356727
    36. Data show that microRNA miR-221 contributes to breast cancer tumorigenicity by regulating stemness, at least in part through the control of DNA methyltransferase 3B (DNMT3b) expression. PMID: 26556862
    37. Downregulation of DNMT3B, one of the targets identified using this method, radiosensitizes cancer cells by disturbing multiple DNA damage responses. PMID: 26667181
    38. Our results demonstrate DNMT3B4-del as a critical factor in developing aberrant DNA methylation patterns during lung tumorigenesis and suggest that DNMT3B4-del may be a target for lung cancer prevention. PMID: 26629529
    39. Results suggested that the DNMT3B -149C/T polymorphism is associated with a genetic susceptibility for developing LSCC in a Chinese population. PMID: 26505438
    40. Increasing DNA methylation in the gene-coding area of DNMT3B was associated with higher risk of colorectal adenomas (OR = 1.34; 95% CI: 1.01-1.79 per SD). PMID: 27062459
    41. Deregulated Dnmt3b targets have prognostic impact in acute myeloid leukemia patients. PMID: 26729896
    42. DNMT3B-579G>T polymorphism might contribute to the susceptibility of cancers especially in the Asian population and for colorectal cancer PMID: 26406961
    43. DNMT3b polymorphisms may predict long-term survival of gastric cancer. However, further studies are needed to reveal the underlying biological roles of DNMT3b polymorphism. PMID: 26262893
    44. Our results suggest that DNA (cytosine-5-)-methyltransferase 3 beta polymorphism is involved in the development of colon cancer and non-random genes promoter methylation among Iranian population. PMID: 25769449
    45. For DNMT3B, increasing DNA methylation of CpG sites in the gene-coding area was associated with a higher risk of colorectal adenomas. PMID: 26485042
    46. There were no significant differences in DNMT3b mRNA expression between patients with SLE and healthy controls. PMID: 25899090
    47. Results suggest that DNMT3B polymorphisms may explain variability in the IQ response to either enriched or impoverished environmental conditions. PMID: 25530201
    48. Activated IL-6 signaling might be responsible to the induction of DNMT3b overexpression on oral cancer PMID: 24625449
    49. The inability to downregulate DNMT3B expression in Endometriosis may contribute to an aberrant epigenetic fingerprint that misdirects gene expression in endometriosis and contributes to its altered response to steroid hormones. PMID: 26239024
    50. low levels of miR-29c in the CSF were positively correlated with the protein expression of BDNF and negatively correlated with the protein expression of DNMT3 in patients with AD. PMID: 25815896

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  • 相關(guān)疾?。?/div>
    Immunodeficiency-centromeric instability-facial anomalies syndrome 1 (ICF1); Facioscapulohumeral muscular dystrophy 2 (FSHD2)
  • 亞細(xì)胞定位：
    Nucleus.
  • 蛋白家族：
    Class I-like SAM-binding methyltransferase superfamily, C5-methyltransferase family
  • 組織特異性：
    Ubiquitous; highly expressed in fetal liver, heart, kidney, placenta, and at lower levels in spleen, colon, brain, liver, small intestine, lung, peripheral blood mononuclear cells, and skeletal muscle. Isoform 1 is expressed in all tissues except brain, s
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 2979

    OMIM: 158901

    KEGG: hsa:1789

    STRING: 9606.ENSP00000328547

    UniGene: Hs.643024