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GPER1 Antibody

  • 中文名稱：
    GPER1兔多克隆抗體
  • 貨號(hào)：
    CSB-PA934518
  • 規(guī)格：
    ￥1100
  • 圖片：
    • The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA934518(GPER1 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: ×200)
    • Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-3: Human placenta tissue, HepG2 cells, A549 cells, Primary antibody: CSB-PA934518(GPER1 Antibody) at dilution 1/350, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 minutes
  • 其他：

產(chǎn)品詳情

  • Uniprot No.：
  • 基因名：
  • 別名：
    GPER1; CEPR; CMKRL2; DRY12; GPER; GPR30; G-protein coupled estrogen receptor 1; Chemoattractant receptor-like 2; Flow-induced endothelial G-protein coupled receptor 1; FEG-1; G protein-coupled estrogen receptor 1; G-protein coupled receptor 30; GPCR-Br; IL8-related receptor DRY12; Lymphocyte-derived G-protein coupled receptor; LYGPR; Membrane estrogen receptor; mER
  • 宿主：
    Rabbit
  • 反應(yīng)種屬：
    Human,Mouse,Rat
  • 免疫原：
    Synthetic peptide of Human GPER1
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 抗體亞型：
    IgG
  • 純化方式：
    Antigen affinity purification
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA,WB,IHC
  • 推薦稀釋比：
    Application Recommended Dilution
    ELISA 1:1000-1:2000
    WB 1:200-1:1000
    IHC 1:25-1:100
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    G-protein coupled estrogen receptor that binds to 17-beta-estradiol (E2) with high affinity, leading to rapid and transient activation of numerous intracellular signaling pathways. Stimulates cAMP production, calcium mobilization and tyrosine kinase Src inducing the release of heparin-bound epidermal growth factor (HB-EGF) and subsequent transactivation of the epidermal growth factor receptor (EGFR), activating downstream signaling pathways such as PI3K/Akt and ERK/MAPK. Mediates pleiotropic functions among others in the cardiovascular, endocrine, reproductive, immune and central nervous systems. Has a role in cardioprotection by reducing cardiac hypertrophy and perivascular fibrosis in a RAMP3-dependent manner. Regulates arterial blood pressure by stimulating vasodilation and reducing vascular smooth muscle and microvascular endothelial cell proliferation. Plays a role in blood glucose homeostasis contributing to the insulin secretion response by pancreatic beta cells. Triggers mitochondrial apoptosis during pachytene spermatocyte differentiation. Stimulates uterine epithelial cell proliferation. Enhances uterine contractility in response to oxytocin. Contributes to thymic atrophy by inducing apoptosis. Attenuates TNF-mediated endothelial expression of leukocyte adhesion molecules. Promotes neuritogenesis in developing hippocampal neurons. Plays a role in acute neuroprotection against NMDA-induced excitotoxic neuronal death. Increases firing activity and intracellular calcium oscillations in luteinizing hormone-releasing hormone (LHRH) neurons. Inhibits early osteoblast proliferation at growth plate during skeletal development. Inhibits mature adipocyte differentiation and lipid accumulation. Involved in the recruitment of beta-arrestin 2 ARRB2 at the plasma membrane in epithelial cells. Functions also as a receptor for aldosterone mediating rapid regulation of vascular contractibility through the PI3K/ERK signaling pathway. Involved in cancer progression regulation. Stimulates cancer-associated fibroblast (CAF) proliferation by a rapid genomic response through the EGFR/ERK transduction pathway. Associated with EGFR, may act as a transcription factor activating growth regulatory genes (c-fos, cyclin D1). Promotes integrin alpha-5/beta-1 and fibronectin (FN) matrix assembly in breast cancer cells.
  • 基因功能參考文獻(xiàn)：
    1. serum levels of GPER, but not estrogen, are significantly decreased in ADHD patients compared to controls PMID: 29659348
    2. The findings indicate that GPER/miR148a/HLAG signaling pathway may mediates the development of ovarian endometriosis and may become a potential therapeutic target for the treatment of endometriosis. PMID: 29845209
    3. Nuclear GPR30 is overexpressed and predicts poor survival in patients with ovarian cancer. PMID: 29239277
    4. These findings shed new light on the essential role played by GPER in IGF1/IGF1R signaling that induces breast tumor angiogenesis. PMID: 29212519
    5. The significant and consistent increase in GPER expression in adenomyosis compared with control subjects, regardless of whether it was in the proliferative or secretory phases and regardless of whether it was in the JZ or OM, suggests that GPER plays an important role in the pathogenesis of the adenomyosis PMID: 29109960
    6. Levels of GPR30 were significantly reduced in placentae from women with preeclampsia as compared with uncomplicated pregnancies. PMID: 28849224
    7. High expression of GPER is associated with triple-negative breast cancer. PMID: 28535016
    8. Serum GPR30 levels were significantly lower in autism spectrum disorders patients than in controls. PMID: 28734238
    9. GPER P16L is defective for membrane-associated signaling, but instead acts like an estrogen-stimulated transcription factor. In CAFs, it induces the secretion of paracrine factors that promote the migration of carcinoma cells. This raises the possibility that the GPER P16L polymorphism could be a risk factor for breast cancer. PMID: 28596490
    10. These data thus demonstrate that estrogen prevents the failure of endothelial cell tube formation induced byhypoxia/reoxygenation. GPR30 plays an important role in these protective effects through the activation of eNOS and Akt in endothelial cells. PMID: 28440394
    11. Epigenetic down regulation of GPER acts as a tumor suppressor in colorectal cancer and its specific activation might be a potential approach for colorectal cancer treatment. PMID: 28476123
    12. Data suggest that IGF-I/IGF-IR system triggers stimulatory actions through both GPER and DDR1 in aggressive tumors as mesothelioma and lung tumors. PMID: 27384677
    13. The G protein-coupled estrogen receptor agonist G-1 inhibits nuclear estrogen receptor activity and stimulates novel phosphoproteomic signatures in MCF-7 cells. PMID: 27026707
    14. Utilizing both genetic and pharmacologic approaches, the authors establish that sex steroid effects on human melanin synthesis are mediated by the membrane-bound, steroid hormone receptors G protein-coupled estrogen receptor (GPER), and progestin and adipoQ receptor 7 (PAQR7). PMID: 27115344
    15. This study demonstrated that GPER1 levels were associated with the anxiety levels of patients, and that the serum GPER1 level was a valuable predictor of the presence of anxiety independent of gender. PMID: 27512921
    16. These clinical data showed that the expression of G-protein coupled estrogen receptor (GPER) is negatively associated with lymph node metastasis, high-grade tumor and fibronectin (FN) expression while positively associated with the favorable outcome in 135 triple negative breast cancer cells patients. PMID: 26842883
    17. Either by specific inhibitors for GPER, ERK, AKT and NF-kappaB, or by knock-down of GPER. PMID: 27940299
    18. The intron variant rs4265085 of GPER1 may confer risk for recurrent spontaneous abortion in Dai and Bai ethnic groups in China. PMID: 28126236
    19. Activation of GPER can suppress migration and angiogenesis of triple negative breast cancer by inhibiting of NF-kappaB/IL-6 signaling. PMID: 27836733
    20. GPER protects against hepatic tumorigenesis by regulating inflammatory responses. PMID: 27594673
    21. GPER enhances melanogenesis via PKA by upregulating microphthalmia-related transcription factor-tyrosinase in melanoma PMID: 27378491
    22. Data suggest that GPR30 increases ERK1/2 activity via two Gi/o-mediated mechanisms, a PDZ-dependent constitutive mechanism and a PDZ-independent Gi/o-stimulated mechanism involving PI3K. (GPR30 = G protein-coupled estrogen receptor 1; ERK1 = extracellular signal-regulated kinase 1; ERK2 = extracellular signal-regulated kinase 2; Gi/0 = GTP-binding protein alpha subunits, Gi-Go; PI3K = phosphoinositide-3-kinase) PMID: 28450397
    23. present study revealed that BPA can trigger the progression of laryngeal squamous cell carcinoma via GPER-mediated upregulation of IL-6. Therefore, more attention should be paid for the BPA exposure on the development of laryngeal cancer. PMID: 28466560
    24. G protein-coupled estrogen receptor stabilizes HIF-1alpha and thus promotes HIF-1alpha-induced VEGF and MMP9 in endometrial stromal cells, which play critical roles in endometriosis. PMID: 27939762
    25. GPR30 activation by G-1 interfered expression of cell cycle regulators and machinery elements to modulate prostate cancer cell growth PMID: 27908592
    26. These results provide evidence that (1) GPR30 is involved in regulating cell proliferation and apoptosis; (2) pharmacologic upregulation of GPR30 is beneficial for preeclampsia (PE) management; (3) GPR30 may therefore be an interventional target for pregnancies complicated by PE. PMID: 27481507
    27. Thus, the presence or absence of these GPR30 species is a simple and rapid manner to determine whether a given cell line is suitable for pharmacological or molecular studies of GPR30 modulation. PMID: 27401115
    28. Data define novel insights into the stromal GPER-mediated multiple drug resistance from the point of reprogramming of tumor energy metabolism and provide the rationale for CAFs as a promising target for clinical therapy. PMID: 27721408
    29. GPER may play a role during human oocyte maturation through its action in cumulus granulosa cells PMID: 27111051
    30. ligand-activation of GPER generates a feedforward loop coupling IL1beta induction by CAFs to IL1R1 expression by cancer cells, promoting the up-regulation of IL1beta/IL1R1 target genes such as PTGES, COX2, RAGE and ABCG2 PMID: 27072893
    31. Studies identified an important role of GPER activation in the regulation of cardiovascular function especially in women. [review] PMID: 27213340
    32. GPER negatively regulates TNFalpha-induced IL-6 expression, probably through inhibition of NF-kappaB promoter activity by a signal(s) derived from the C-terminal region of GPER. PMID: 26888479
    33. Results demonstrated that GPER protein down-regulation significantly correlated with GPER promoter hypermethylation. Comparison of 108 tumors and matched normal breast tissues indicated a significant GPER down-regulation in cancer tissues correlating with GPER promoter hypermethylation. PMID: 28118074
    34. Data suggest that expression of GPER1/GPR30 can be up-regulated by dietary factors; dietary supplementation with flaxseed-derived lignan, secoisolariciresinol diglycoside, up-regulates expression of GPER1/GPR30 in prostate and may prevent progression of benign prostatic hyperplasia. PMID: 27849354
    35. the presence of the GG genotype of the GPR30 rs3808351 polymorphism and the G allele of the GPR30 rs3808351 polymorphism affect the characteristics and development of leiomyomas in the Turkish population PMID: 26773178
    36. GPER expression was mainly con fi ned in the basal epithelial layer of benign prostate, where this receptor could mediate estrogen action on normal cellular activity. PMID: 26714890
    37. Data show that both mineralcorticoid receptor (MR) and G-protein estrogen receptor (GPER) contribute to the proliferation and migration of breast and endothelial cancer cells by sodium-hydrogen exchanger 1 protein (NHE-1) upon aldosterone exposure. PMID: 26646587
    38. GPER ligand-independently stimulates the proliferation, migration and invasion of SKOV3 cells. PMID: 26526233
    39. Study provides perspective that addresses the accumulation of GPER in endosomes or intracellular membranes, its capacity to transactivate plasma membrane receptors and its potential role in physiological and pathophysiological processes. [review] PMID: 26190834
    40. Evaluation of GPER1, EGFR and CXCR1 mRNA/protein expression may be helpful in differential diagnosis of malignant follicular thyroid carcinoma and benign follicular thyroid adenoma. PMID: 26617848
    41. Results indicate that GPER-1 mediates proliferation of estrogen-induced leiomyoma cells by activating the MAPK pathway, and not by promoting mitosis. PMID: 26416628
    42. GPER significantly attenuated the inhibition effect of miR-424 in estradiol-induced cell growth in the endometrial cancer cells. PMID: 26638889
    43. The G protein-coupled estrogen receptor 1 (GPER-1) contributes to the proliferation and survival of mantle cell lymphoma cells. PMID: 26250574
    44. Understanding the molecular basis of agonist/antagonist mechanism of GPER1/GPR30 through structural and energetic analyses PMID: 26772481
    45. Expression and functional roles of estrogen receptor GPR30 in human intervertebral disc PMID: 26815911
    46. Data show that estrogen mediates control of hepatitis C virus through the G-protein-coupled estrogen receptor 30 (GPR30) pathway leading to cleavage of occludin by Matrix Metalloproteinase-9 (MMP-9). PMID: 26731262
    47. a significant positive correlation was found between GPER and Gankyrin both in ectopic and eutopic endometrium of the ovarian endometriosis. PMID: 26193952
    48. In estradiol-treated monocytes, GPER1 physically interacts with estrogen receptor-alpha 36-kDa splice variant. It acts an anti-inflammatory coregulator, because its inhibition blocks estrogen's effect on IL-6 expression. PMID: 26394816
    49. Study highlighted the physiological function of GPER1, particularly its regulation of AT1R and the role of estrogen receptors, EGFR and matrix metalloproteinases in bringing about its cardioprotective effects. [review] PMID: 25922871
    50. GPER undergoes dramatic structural changes, which explains its exceptional capacity to accept diverse agonist and antagonist ligands. PMID: 25587872

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  • 亞細(xì)胞定位：
    Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Cytoplasm, cytoskeleton. Cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein. Early endosome. Recycling endosome. Golgi apparatus membrane; Multi-pass membrane protein. Golgi apparatus, trans-Golgi network. Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell projection, dendrite. Cell projection, dendritic spine membrane; Multi-pass membrane protein. Cell projection, axon. Cell junction, synapse, postsynaptic density. Mitochondrion membrane; Multi-pass membrane protein.
  • 蛋白家族：
    G-protein coupled receptor 1 family
  • 組織特異性：
    Expressed in placenta, endothelial and epithelial cells, non laboring and laboring term myometrium, fibroblasts and cancer-associated fibroblasts (CAF), prostate cancer cells and invasive adenocarcinoma (at protein level). Ubiquitously expressed, but is m
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 4485

    OMIM: 601805

    KEGG: hsa:2852

    STRING: 9606.ENSP00000297469

    UniGene: Hs.20961