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  4. HK2 Antibody

HK2 Antibody

  • 中文名稱：
    HK2兔多克隆抗體
  • 貨號：
    CSB-PA611310
  • 規(guī)格：
    ￥1100
  • 圖片：
    • The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using CSB-PA611310(HK2 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200)
    • The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA611310(HK2 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200)
  • 其他：

產(chǎn)品詳情

  • Uniprot No.：
  • 基因名：
  • 別名：
    DKFZp686M1669 antibody; Hexokinase 2 antibody; Hexokinase 2 muscle antibody; Hexokinase type II antibody; Hexokinase-2 antibody; HK 2 antibody; HK II antibody; HK2 antibody; HKII antibody; HxK 2 antibody; HxK2 antibody; HXK2_HUMAN antibody; Muscle form hexokinase antibody
  • 宿主：
    Rabbit
  • 反應(yīng)種屬：
    Human,Mouse,Rat
  • 免疫原：
    Fusion protein of Human HK2
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 抗體亞型：
    IgG
  • 純化方式：
    Antigen affinity purification
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA,IHC
  • 推薦稀釋比：
    Application Recommended Dilution
    ELISA 1:1000-1:5000
    IHC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

產(chǎn)品評價

靶點詳情

  • 功能：
    Catalyzes the phosphorylation of hexose, such as D-glucose and D-fructose, to hexose 6-phosphate (D-glucose 6-phosphate and D-fructose 6-phosphate, respectively). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate. Plays a key role in maintaining the integrity of the outer mitochondrial membrane by preventing the release of apoptogenic molecules from the intermembrane space and subsequent apoptosis.
  • 基因功能參考文獻：
    1. downregulation of HK2 gene may be caused by its negative regulation through microRNAs miR-9-5p, -98-5p, and -199-5p. PMID: 29695690
    2. this study reports the structure of HK2 in complex with glucose and glucose-6-phosphate (G6P). PMID: 29298880
    3. Altogether, these findings provide strong support for the direct contribution of HK2 in B-cell lymphoma development and suggest that HK2 is a key metabolic driver of the diffuse large B-cell lymphoma phenotype. PMID: 29335581
    4. Taken together, these findings suggested that UCA1 may have an important role in regulating radioresistance through the HK2/glycolytic pathway, providing novel potential targets to improve cervical cancer RT. PMID: 30015920
    5. Data suggest that HK2 plays role in cisplatin resistance in ovarian cancer by regulating drug-induced, MAP kinase signaling-mediated autophagy. PMID: 29247711
    6. miR-125b-5p-HK2 pathway as a novel mechanism in regulating the glycolysis and progression of Laryngeal squamous cell carcinoma. PMID: 29864898
    7. These findings provide clues regarding the role of miR-216a-5p as a tumor suppressor in uveal melanoma through the inhibition of HK2. PMID: 29763606
    8. Overexpression of miR-125b inhibits cellular glucose metabolism through direct targeting of hexokinase 2. PMID: 29631677
    9. Data indicate that hexokinase-2 (HK2) was the direct target of miR-125b. PMID: 29169422
    10. Tesults suggest that mTOR-STAT3-HK2 pathway is involved in the glycolysis of HCC cells and STAT3 may regulate HCC glycolysis through HK2 pathway. PMID: 28445971
    11. our results demonstrate that deregulation of HK2 expression has an important function in the progression of TSCC and may serve as a biomarker of its metastatic potential in TSCC patients. HK2 enhances the metastatic potential of TSCC by stimulating the SOD2-H2O2 pathway. PMID: 27926482
    12. present studies highlight miR-143 as a tumor suppressor in oral squamous cell carcinoma (OSCC) by the suppression of cell migration, glucose metabolism and proliferation through directly targeting HK2, rendering miR-143 a therapeutic strategy for the treatment of clinical OSCC patients. PMID: 28174335
    13. FOXM1 bound directly to the GLUT1 and HK2 promoter regions and regulated the promoter activities and the expression of the genes at the transcriptional level. This reveals a novel mechanism by which glucose metabolism is regulated by FOXM1. PMID: 27351131
    14. our findings supported a mechanism whereby targeting HK2 inhibition contributed to suppress HPV16 E7-induced tumor glycolysis metabolism phenotype, inhibiting tumor growth, and induced apoptosis, blocking the cancer cell energy sources and ultimately enhanced the sensitivity of HPV(+) cervical cancer cells to irradiation therapy PMID: 28498475
    15. Data suggest Hexokinase 2 (HK2) as a potentially effective therapeutic target in Glioblastoma (GBM). PMID: 27588472
    16. High HKII expresiion is associated with nasopharyngeal carcinoma. PMID: 26848773
    17. Results show that the hexokinase 2 (HK2) promoter CpG island (HK2-CGI) surrounding regions N-shore was hypomethylated, thereby enhancing HK2 expression, the HK2-CGI was itself hypermethylated in some hepatocellular carcinomas (HCCs). PMID: 27260001
    18. findings confirmed the tumorigenic activity of HK2 in glioma, and the involvement of the miR218/Bmi1 pathway in the regulation of its expression PMID: 29220380
    19. BAG3 directly stabilizes hexokinase 2 mRNA and promotes aerobic glycolysis in pancreatic cancer cells. PMID: 29114069
    20. Previously unknown reciprocal influence of SIRT6 and HK2 in regulating autophagy driven monocyte differentiation. PMID: 28935467
    21. SIRT6 over-expression establishes a condition whereby reconfiguration of the Hexokinase 2 promoter chromatin structure makes it receptive to interaction with MZF1/SIRT6 complex, thereby favouring a regulatory state conducive to diminished transcription PMID: 28478957
    22. findings suggested that PVT1 contributes to osteosarcoma (OS) cell glucose metabolism, cell proliferation, and motility through the miR-497/HK2 pathway, and revealed a novel relation between lncRNA and the alteration of glycolysis in OS cells PMID: 28602700
    23. High HKII expression is associated with metastasis and epithelial-mesenchymal transition in hepatocellular carcinoma. PMID: 28429188
    24. The EML4-ALK-mediated upregulation of HIF1alpha, HK2 and glycolytic metabolism was also highly active in vivo as demonstrated by fluorodeoxyglucose-positron emission tomography imaging of xenografts grown from EML4-ALK-positive NSCLC cells. PMID: 27132509
    25. relevant number of patients with FDG-PET false-negative multiple myeloma and a strong association between hexokinase-2 expression and this negativity: a finding which may also be relevant for clinical imaging of other hematological cancers. PMID: 28432222
    26. MicroRNA-98 Suppress Warburg Effect by Targeting HK2 in Colon Cancer Cells. PMID: 28025745
    27. High HK2 expression is associated with prostate cancer. PMID: 28098871
    28. Metformin downregulated both endogenous and exogenous UCA1 expression, leading to the inhibition of mammalian target of rapamycin-signal transducer and activator of transcription 3-hexokinase 2 signaling pathway. Our study provided the first evidence that metformin inhibited proliferation and glycolysis in cancer cells through regulation of long non-coding RNA UCA1 PMID: 28641488
    29. AKT inhibition blocks miR-124 silencing-induced AKT1/2, glucose transporter 1, hexokinase II activation, cell proliferation, and glycolytic or energy metabolism changes. In summary, this study demonstrated that miR-124 is able to inhibit proliferation, glycolysis, and energy metabolism, potentially by targeting AKT1/2-glucose transporter 1/hexokinase II in non-small cell lung cancer cells. PMID: 28488541
    30. Results from the simulations show that HK2 binding restricts the movement of the VDAC1 N-terminal helix. As a result, VDAC1 is kept in the open state most of the time and probably allows a constant supply of ATP to HK2 for glycolysis. PMID: 27544294
    31. These results suggest that HK2 plays important roles in the glycolytic, proliferation and migration properties of colorectal carcinoma PMID: 27363977
    32. HK2modulated glucose metabolism was significantly upregulated by overexpression of miR155. Notably, inhibition of miR155 sensitized lung cancer cells to irradiation via suppression of glucose metabolism. PMID: 27315591
    33. We demonstrated that the expression levels of glycolysis-related proteins glucose transporter 1, hexokinase II, carbonic anhydrase IX, and monocarbonylate transporter 4 differ between thyroid cancer subtypes and are correlated with poorer prognosis PMID: 28347233
    34. miR-181b directly inhibits the expression level of hexokinase 2 (HK2), a key enzyme that catalyzes the first step of glycolysis, through targeting its 3'-untranslated region. PMID: 27314295
    35. The N-terminal end of HKII has a mitochondrial-binding site and a VDAC bindig site. PMID: 26182367
    36. HK2 functions as a novel oncogene in lung cancer and may be a potential therapeutic target for lung cancer. PMID: 26802651
    37. Cells with HKII knockdown showed no changes in 3BP sensitivity, suggesting the effects of 3BP are independent of HKII expression. PMID: 26740252
    38. Suggest HK2 as an independent prognostic indicator of radiation resistance in cervical squamous carcinoma. PMID: 26097593
    39. High exokinase 2 expression is associated with neuroblastoma metastasis. PMID: 26986252
    40. The degree of plaque disruption and expression of TF and HK-II appear to be important vascular factors for AMI onset, and polarized macrophages make a distinct contribution to thrombogenicity and glucose metabolism. PMID: 26346032
    41. The overexpression of Hk2 (and Pkm2) sustains high levels of glycolysis during embryonic stem cell differentiation. PMID: 26059508
    42. In the present study it was determined whether three key enzymes for glycolysis, glutaminolysis and de novo synthesis of FAs, hexokinase-2, glutaminase and fatty acid synthase PMID: 26134042
    43. In conclusion, our findings suggested that miR-4458 inhibited the progression of colon cancer cells by inhibition of glycolysis and lactate production via directly targeting HK2 mRNA. PMID: 26607110
    44. Hexokinase 2 controls cellular stress response through localization of hnRNP A1. PMID: 26247723
    45. Insulin resistance in obese boys leads to up-regulation of INSIG2 gene expression as well as to down-regulation of PFKFB1, PFKFB3, and HK2 genes in the blood cells as compared to obese patients with normal insulin sensitivity. PMID: 26827442
    46. HK2 was identified as a chaperone-mediated autophagy substrate. PMID: 26323688
    47. study reveals a novel relationship between PI3K/Akt signaling and aerobic glycolysis and indicates that PI3K/Akt/HK2 might be potential therapeutic approaches for OS PMID: 26116768
    48. HK2 regulated the protein level and T14 phosphorylation of CDK2, and knockdown of HK2 resulted in a G1 phase cell cycle arrest. PMID: 24780297
    49. High HK-II expression was correlated with esophageal adenocarcinoma compared with squamous cell carcinoma. PMID: 24939624
    50. Suggest that upregulation of HKII occurs during the process of hepatocarcinogenesis in humans. PMID: 25381201

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  • 亞細(xì)胞定位：
    Mitochondrion outer membrane; Peripheral membrane protein. Cytoplasm, cytosol.
  • 蛋白家族：
    Hexokinase family
  • 組織特異性：
    Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 4923

    OMIM: 601125

    KEGG: hsa:3099

    STRING: 9606.ENSP00000290573

    UniGene: Hs.406266