Immunofluorescence staining of Hela cells with CSB-PA529743LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in specific signaling in the thyroid gland.
基因功能參考文獻:
Single-nucleotide polymorphism in PDE8B gene is associated with Hashimoto's thyroiditis. PMID: 28382505
replication confirmed at genome-wide significance the association of loci at FOXE1 with hypothyroidism, and PDE8B, CAPZB and PDE10A with serum TSH. A total of 12 SNPs seemed to explain nearly 7% of the serum TSH variation PMID: 28727628
a heterozygous nonsense mutation in the first exon of cyclic nucleotide phosphodiesterase 8B gene, which is predicted to disrupt all important functional domains of the cyclic nucleotide phosphodiesterase 8B protein, in two members of family with autosomal-dominant striatal degeneration PMID: 26769607
Genetic variation of the PDE8B gene may be involved in the etiology of subclinical hypothyroidism in pregnant women. PMID: 25822812
A prevalence of the minor allele of PDE8B gene polymorphism associated with elevated serum levels of TSH was demonstrated in patients affected by sporadic nonautoimmune subclinical hypothyroidism. PMID: 24497218
rs4704397 is associated with thyroid function, risk of MI, and body height. PMID: 23941514
rs4704397 in phosphodiesterase 8B is associated with thyrotropin and thyroid hormone concentrations PMID: 23272636
There is an association between homozygous A/A as well as homozygous G/G carriers of SNP rs 4704397 in PDE8B and recurrent miscarriage. PMID: 23237535
PDE8B gene polymorphisms may be correlated with hyperthyroxinemia in the Chinese Han population. PMID: 22781450
PDE8B is another PDE gene in which variations may contribute to predisposition of adrenocortical tumours. PMID: 22335482
In obese children, PDE8B is associated with TSH; the interaction between adiposity and PDE8B on TSH is not synergistic, but follows an additive model. PMID: 22084153
Common genetic variation in PDE8B is associated with reciprocal changes in thyroid hormone levels. PMID: 21317282
Our analysis revealed separate segregation of an inactivating PDE8B allele from the high-TSH-allele and showed low TSH levels in persons who carry an inactivating PDE8B allele. These data suggest that PDE8B may be involved in regulation of TSH levels. PMID: 20373981
phosphodiesterase 8B has a role in autosomal-dominant striatal degeneration PMID: 20085714
selective usage of exons produces three different PDE8B variants that exhibit a tissue-specific expression pattern PMID: 12372422
Comparison of enzymatic characterization and gene organization of PDE8B and PDE8A. PMID: 12681444
In Alzheimer's disease brains we found that PDE8B was the only PDE isozyme showing a significant increase, in cortical areas and parts of the hippocampal formation, at Braak stages III-VI PMID: 12895443
PDE8B is another PDE gene linked to isolated micronodular adrenocortical disease; it is a candidate causative gene for other adrenocortical lesions linked to the cAMP signaling pathway and possibly for tumors in other tissues. PMID: 18431404
results suggest a primary effect of PDE8B variants on cAMP levels in the thyroid. This would affect production of T4 and T3 and feedback to alter TSH release by the pituitary. PMID: 18514160
Genetic variation in thyroid stimulating hormone levels in pregnancy associated with the PDE8B rs4704397 genotype has implications for the number of women treated for subclinical hypothyroidism under current guidelines PMID: 19820008
Abundantly expressed in the thyroid. Also very weakly expressed in brain, spinal cord and placenta. In the thyroid isoform 1 predominates, and isoforms 2 and 6 are also highly expressed. In the placenta isoforms 1 and 2 are expressed equally. In the brain