PNPLA5 Antibody
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中文名稱:PNPLA5兔多克隆抗體
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貨號:CSB-PA773803LA01HU
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規(guī)格:¥440
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促銷:
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圖片:
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Western Blot
Positive WB detected in: Colo320 whole cell lysate, A549 whole cell lysate
All lanes: PNPLA5 antibody at 3.1µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 48, 36 kDa
Observed band size: 48 kDa -
IHC image of CSB-PA773803LA01HU diluted at 1:500 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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IHC image of CSB-PA773803LA01HU diluted at 1:500 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunofluorescence staining of HepG2 cells with CSB-PA773803LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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其他:
產品詳情
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產品名稱:Rabbit anti-Homo sapiens (Human) PNPLA5 Polyclonal antibody
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Uniprot No.:
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基因名:PNPLA5
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別名:PNPLA5 antibody; GS2L antibody; Patatin-like phospholipase domain-containing protein 5 antibody; EC 3.1.1.- antibody; GS2-like protein antibody
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宿主:Rabbit
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反應種屬:Human
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免疫原:Recombinant Human Patatin-like phospholipase domain-containing protein 5 protein (90-205AA)
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免疫原種屬:Homo sapiens (Human)
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標記方式:Non-conjugated
本頁面中的產品,PNPLA5 Antibody (CSB-PA773803LA01HU),的標記方式是Non-conjugated。對于PNPLA5 Antibody,我們還提供其他標記。見下表:
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克隆類型:Polyclonal
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抗體亞型:IgG
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純化方式:>95%, Protein G purified
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濃度:It differs from different batches. Please contact us to confirm it.
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保存緩沖液:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 -
產品提供形式:Liquid
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應用范圍:ELISA, WB, IHC, IF
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推薦稀釋比:
Application Recommended Dilution WB 1:500-1:5000 IHC 1:500-1:1000 IF 1:50-1:200 -
Protocols:
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儲存條件:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期:Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
相關產品
靶點詳情
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功能:Has abundant triacylglycerol lipase activity.
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基因功能參考文獻:
- required for optimal autophagy initiation PMID: 24613307
- No association between genetic variants in PNPLA5 genes and childhood and adolescent obesity PMID: 19390624
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組織特異性:Expressed in brain and pituitary gland.
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數據庫鏈接:
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