Immunohistochemistry of paraffin-embedded human prostate cancer using CSB-PA13669A0Rb at dilution of 1:100
Immunofluorescence staining of HepG2 cells with CSB-PA13669A0Rb at 1:266, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
DYT16 antibody; HSD14 antibody; Interferon inducible double stranded RNA dependent protein kinase activator A antibody; interferon-inducible double stranded RNA-dependent activator antibody; Interferon-inducible double stranded RNA-dependent protein kinase activator A antibody; PACT antibody; PKR associated protein X antibody; PKR associating protein X antibody; PKR-associated protein X antibody; PKR-associating protein X antibody; PRKRA antibody; PRKRA_HUMAN antibody; Protein activator of the interferon induced protein kinase antibody; Protein activator of the interferon-induced protein kinase antibody; Protein kinase antibody; Protein kinase interferon inducible double stranded RNA dependent activator antibody; RAX antibody
宿主:
Rabbit
反應(yīng)種屬:
Human
免疫原:
Recombinant Human Interferon-inducible double-stranded RNA-dependent protein kinase activator A protein (1-313AA)
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貨期:
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1. Promotes UBC9-p53/TP53 association and sumoylation and phosphorylation of p53/TP53 at 'Lys-386' at 'Ser-392' respectively and enhances its activity in a EIF2AK2/PKR-dependent manner.
基因功能參考文獻(xiàn):
Low PACT expression is associated with Arenaviral infections. PMID: 29669840
phenotype of our patients overlaps that of previouslyreported DYT16 patients PMID: 26990861
this study shows that PACT is an essential coactivator of the MDA5-dependent type I IFN response to viral RNA PMID: 28760879
High PRKRA mRNA expression is associated with colorectal cancer. PMID: 27859935
It was established in this report that interactions between PACT, ADAR1 and HIV-1-encoded Tat protein diminish the activation of PKR in response to HIV-1 infection. PMID: 28167698
5 genomic variants in GSC, HOXA2 and PRKRA were identified through mutational analysis in Chinese patients with microtia. PMID: 28109504
MicroRNA-122 Inhibits the Production of Inflammatory Cytokines by Targeting the PKR Activator PACT in Human Hepatic Stellate Cells. PMID: 26636761
The interferon-induced protein kinase(PACT) partially rescued defects of interferon-beta1 and chemokine (C-C motif) ligand 5/RANTES (regulated on activation normal T cell expressed and secreted) induction in DDX3-knockdown HEK293 cells. PMID: 26454002
These findings support a model in which a measles virus defective interfering RNA is sensed by PACT and RIG-I to initiate an innate antiviral response via activation of interferon-beta production. PMID: 26608320
this study demonstrates for the first time that OV20.0 of Orf virus is also able to interact with the dsRNA binding domain of PACT and that the presence of dsRNA strengthened the interaction of these two molecules. PMID: 26355092
the affinity of PACT-PACT and PACT-PKR interactions is enhanced in dystonia patient lymphoblasts, thereby leading to intensified PKR activation and enhanced cellular death. PMID: 26231208
Altered PRKRA signalling and C / EBPbeta expression is associated with HLA-B27 expression in monocytic cells. PMID: 21988375
This stuidy provides the first independent replication of the DYT16 locus at 2q31.2 and strongly confirms the causal contribution of the PRKRA gene to DYT16. Our data suggest worldwide involvement of PRKRA in dystonia. PMID: 25142429
NS1 prevents PACT from interacting with an essential component of the virus. PMID: 24899174
PKR is activated in uninfected cells, specifically during mitosis, by binding to dsRNAs formed by inverted Alu repeats (IRAlus). PMID: 24939934
In contrast to its previously described activity, PACT contributes to PKR dephosphorylation during HIV-1 replication. PMID: 24020926
MERS-CoV 4a protein interacted with PACT in an RNA-dependent manner but not with RIG-I or MDA5. PMID: 24522921
Ebola virus VP35 inhibits PACT-induced RIG-I ATPase activity in a dose-dependent manner. PMID: 23870315
Herpes simplex virus 1 Us11 suppresses host interferon beta1 production through the inhibition of the PACT. PMID: 24067967
Protein kinase R modulates c-Fos and c-Jun signaling to promote proliferation of hepatocellular carcinoma with hepatitis C virus infection. PMID: 23844083
in vitro binding patterns of human TRBP and PACT to siRNA PMID: 23658827
The results show that PACT and TRBP have distinct effects on Dicer-mediated dsRNA processing. PMID: 23661684
RISC proteins PACT, TRBP, and Dicer, together with PKR, are steroid receptor RNA activator-binding nuclear receptor coregulators that are recruited to the promoters of hormone-regulated genes and regulate the expression of downstream target genes. PMID: 23550157
PACT may be associated with the plasma cell function and eosinophilic inflammation in CRSwNP. PMID: 22961479
DGCR8, AGO1, AGO2, PACT, and TARBP1 expression levels were significantly higher in the epithelial skin cancer groups than the healthy controls (P > 0.05). PMID: 22025453
The PACT-protein kinase PKR pathway represents a potential link between Abeta accumulation, PKR activation and tau phosphorylation. PMID: 21790829
Data demonstrate that PACT-PACT interaction is essential for efficient PKR activation. PMID: 22473766
NF-kappa-B is involved in the protein activator of PKR-RNA-dependent protein kinase interaction and the production of pro-inflammatory cytokines in periodontitis. PMID: 21882225
show that PACT physically binds to the C-terminal repression domain of RIG-I and potently stimulates RIG-I-induced type I interferon production PMID: 21501829
though mutation analysis demonstrated no mutation of PACT in the patients of microtia in the present study, suggest that PACT may play an essential role in mammalian ear development PMID: 21595004
results demonstrate for the first time that stress-induced PACT phosphorylation functions to free PACT from the inhibitory interaction with TRBP and also to enhance its interaction with PKR PMID: 21526770
The C-terminal, third conserved motif of the protein activator PACT plays an essential role in the activation of double-stranded-RNA-dependent protein kinase PMID: 11985496
Us11 protein of herpes simplex virus type 1 can block PKR activation by PACT both in vitro and in vivo PMID: 12368348
RAX, the cellular activator of PKR, has a novel role in synergistically stimulating SV40 large T antigen-dependent gene expression PMID: 12874289
The interaction with Dicer involves the third dsRNA-binding domain (dsRBD) of PACT and the N-terminal region of Dicer containing the helicase motif. Like TRBP, PACT is not required for the pre-microRNA (miRNA) cleavage reaction step. PMID: 16424907
RAX (PRKRA) may function as a negative regulator of growth that is required to activate PKR in response to a broad range of apoptosis-inducing stress. PMID: 16861340
The underlying biochemical mechanism of the activation of PKR via PACT phosphorylation at specific activation domain residues is reported as the major mode of transmission of cellular stress response to PKR. PMID: 16982605
Results show that PACT is expressed ubiquitously in different cell types at varying abundance, and Sp1 is the major transcription factor responsible for PACT promoter activity. PMID: 17125937
Results indicate that human TRBP and PACT directly interact with each other and associate with Dicer to stimulate the cleavage of double-stranded or short hairpin RNA to siRNA. PMID: 17452327
DYT16, a novel young-onset dystonia-parkinsonism disorder, identification of a segregating mutation in the stress-response protein PRKRA. PMID: 18243799
A heterozygous frameshift mutation in PRKRA (DYT16) associated with generalised dystonia in a German patient. PMID: 18420150
TRBP controls PACT activation of PKR, an activity that is reversed by stress. PMID: 18936160
writer's cramp as presenting symptom is not associated with mutations in DYT11, DYT16, but it can be the sole manifestation of DYT1 GAG deletion mutation carriers. PMID: 19441135
Strong protein kinase R (PKR) binding is needed for PACT-mediated PKR activation and phosphorylation in vivo. PMID: 19580324
RAX phosphorylation is required for PKR activation, full protein synthesis inhibition, and rapid apoptosis following stress. PMID: 15299031