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  4. Phospho-LCP2 (Y128) Antibody

Phospho-LCP2 (Y128) Antibody

  • 中文名稱：
    磷酸化-LCP2 (Y128)兔多克隆抗體
  • 貨號：
    CSB-PA009803
  • 規(guī)格：
    ￥880
  • 圖片：
    • Western Blot analysis of K562 cells using Phospho-SLP-76 (Y128) Polyclonal Antibody
  • 其他：

產(chǎn)品詳情

  • Uniprot No.：
  • 基因名：
  • 別名：
    76 kDa tyrosine phosphoprotein antibody; CG8697 antibody; LCP 2 antibody; LCP2 antibody; LCP2_HUMAN antibody; Lymphocyte cytosolic protein 2 antibody; SH2 domain containing leukocyte protein 76 KD antibody; SH2 domain containing leukocyte protein of 76kD antibody; SH2 domain containing leukocyte protein of 76kDa antibody; SH2 domain-containing leukocyte protein of 76 kDa antibody; SLP 76 antibody; SLP 76 tyrosine phosphoprotein antibody; SLP-76 antibody; SLP-76 tyrosine phosphoprotein antibody; SLP76 antibody; SLP76 tyrosine phosphoprotein antibody
  • 宿主：
    Rabbit
  • 反應(yīng)種屬：
    Human,Mouse,Rat
  • 免疫原：
    Synthesized peptide derived from Human SLP-76 around the phosphorylation site of Y128.
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 抗體亞型：
    IgG
  • 純化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    WB, IHC, ELISA
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:100-1:300
    ELISA 1:5000
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    Involved in T-cell antigen receptor mediated signaling.
  • 基因功能參考文獻(xiàn)：
    1. These data are consistent with a model in which bivalent recruitment of a GADS/SLP-76 complex is required for costimulation by CD6. PMID: 28289074
    2. LAT and SLP-76 are randomly dispersed throughout the clusters that form upon T cell receptor engagement. PMID: 27875277
    3. SLP76 is ectopically expressed in chronic lymphocytic leukemia cells where it plays a role in B-cell receptor signaling. PMID: 27443285
    4. findings identify ACK1 as a novel SLP-76-associated protein-tyrosine kinase that modulates early activation events in T cells. PMID: 28188290
    5. Data strongly suggest that chemokine-stimulated associations between Vav1, SLP-76, and ADAP facilitate Rac1 activation and alpha4beta1-mediated adhesion, whereas Pyk2 opposes this adhesion by limiting Rac1 activation. PMID: 26202465
    6. immune cell adaptor SLP-76 binds directly to SUMO-RanGAP1 of cytoplasmic fibrils of the nuclear pore complex, and this interaction is needed for optimal NFATc1 and NF-kappaB p65 nuclear entry in T cells PMID: 26321253
    7. SLP-76 N-terminal tyrosine residues regulate a dynamic signaling equilibrium involving feedback of proximal T-cell receptor signaling PMID: 25316710
    8. analysis of a costimulatory mechanism by which CXCL12 and antigen converge at SLP-76 microcluster formation to enhance T cell responses PMID: 23901140
    9. Multipoint binding of SLP-76 to ADAP facilitates the assembly of SLP-76 microclusters. PMID: 23979596
    10. Data indicate a role for the SAM domain in mediating SLP-76 self-association for T-cell function. PMID: 23935094
    11. Unique modes of regulation of positive and negative feedback pathways in T cells by SLP-76. PMID: 23071622
    12. These findings reveal a novel role for SLP-76 in CXCR4-mediated T lymphocyte trafficking. PMID: 22806433
    13. a novel regulation mechanism of SLP-76 by ubiquitination and proteasomal degradation of activated SLP-76, which is mediated by Ser-376 phosphorylation, leading to down-regulation of TCR signaling. PMID: 22902619
    14. Complementary phosphorylation sites in the adaptor protein SLP-76 promote synergistic activation of natural killer cells. PMID: 22786724
    15. Nef employs a dual mechanism to disturb early TCR signaling by limiting the communication between LAT and SLP-76 PMID: 22802418
    16. both T cell activation and the association between SLP-76 and Nck. After T cell receptor stimulation, SLP-76 was phosphorylated, which enabled the binding of Nck. PMID: 22534133
    17. our studies demonstrate a novel role for the adaptor molecule SLP-76 in regulating HIV-1 infection in T cells PMID: 22323535
    18. Combining regulated deletion of endogenous SLP-76 with transgenic expression of a SLP-76 SH2 domain mutant demonstrates that the SLP-76 SH2 domain is required for peripheral T cell activation and positive selection of thymocytes. PMID: 21949020
    19. The spatial correlation between kinase ZAP70 and adaptor SLP76 microclusters (MC) at the cell periphery and the effects of F-actin on MC assembly, were analysed. PMID: 21887278
    20. findings demonstrate the critical role of SLP-76-mediated signaling in initiating T-cell-directed immune responses both in vitro and in vivo PMID: 21469089
    21. LAT recruits Src homology 2 domain-containing leukocyte 76 kDa protein (SLP-76) following T-cell receptor ligation and membrane translocation of Akt and phosphatidylinositol 3-kinase (PI3K)phosphorylation in Jurkat cells, activating Akt signaling. PMID: 21282515
    22. Results define the composition, stoichiometry and specificity of interactions in the SLP-76, Nck and VAV1 complex, which is crucial for regulation of the actin machinery after T-cell activation. PMID: 20562827
    23. findings reconfigure the TCR signaling pathway by showing SLP-76 back-regulation of ZAP-70, an event that could ensure that signaling components are in balance for optimal T cell activation PMID: 20534575
    24. The results show that Bcr-Abl regulates the actin cytoskeleton and non-apoptotic membrane blebbing via a GADS/Slp-76/Nck1 adaptor protein pathway. PMID: 20079431
    25. Shb links SLP-76 and Vav with the CD3 complex in Jurkat T cells (SLP-76) PMID: 12084069
    26. SLP-76 is essential for NF-kappa B activation and lipid raft translocation of protein kinase C theta and the I kappa B kinase complex. PMID: 12496421
    27. SLP-76 is required for intracellular calcium ion mobilization in response to SDF-1alpha/CXCL12-induced prolonged activation of extracellular signal-related protein kinase in Jurkat T cells. PMID: 12817019
    28. SLP-76 is necessary for T cell receptor stimulation-induced polarization of the T cell's microtubule-organizing center, as it moves toward the interface of the T cell and antigen-presenting cell. PMID: 12847255
    29. Study provides the first data to address the mechanisms controlling SLP-76 transcription by providing evidence for several key cis-regulatory elements in the promoter region. PMID: 14662865
    30. the proline-rich domain in SLP-76 has a role in subcellular localization and T cell function PMID: 14722089
    31. Data suggest that SLP-76 may play a role in signaling pathways by interacting with the p85 subunit of phosphoinositide 3-kinase (PI3K). PMID: 15388330
    32. SLP-76 need not interact with SH3(PLC) to activate PLC-gamma1, and the P-I region of SLP-76 serves a structural role that is sequence-independent and is not directly related to protein-protein interactions PMID: 15623534
    33. Data show that the adaptor molecules LAT and SLP-76 are specifically targeted by Yersinia to inhibit T cell activation. PMID: 15699071
    34. TCR-induced association of Vav3 with SLP-76 is required for its membrane/IS localization and function PMID: 15708849
    35. In T cells all SLP-76 proteins are in a approximately 400 kDa complex with the small adaptor protein Grb2-like adaptor protein Gads. PMID: 16356554
    36. findings show that retinoic acid(RA) induced the expression of SLP-76, which when co-expressed with an RA-induced receptor, c-FMS, enhanced RA-induced cell differentiation and G0 cell cycle arrest PMID: 16439309
    37. The costimulatory effect of CD6 is mediated through phosphorylation-dependent binding of a specific tyrosine residue, 662Y, in its cytoplasmic region to the adaptor SLP-76. PMID: 16914752
    38. The P-I region deletion disrupted Vav association and reduced SLP-76-associated kinase activity. PMID: 17148460
    39. The integrity of T-cell receptor signaling in vivo is sustained both by strong selection of SLP-76 for the Gads C-SH3 domain and by a capacity to buffer intrinsic crossreactivity. PMID: 17235283
    40. phosphorylation of the adaptor molecule SLP-76 is essential for recruitment of the exchange factor Vav leading to Ca(2+) flux and IL-2 production PMID: 17237383
    41. Required for activation of IL-2-inducible T cell kinase (ITK); furthermore, an ongoing physical interaction between SLP-76 and ITK is required to maintain ITK in an active conformation. PMID: 17420479
    42. SLP-76 relocalizes to integrin-initiated signaling complexes by a mechanism different from that employed during TCR signaling and that SLP-76 relocalization corresponds to SLP-76-dependent integrin function in T cells. PMID: 19667077
    43. SLP76 is differentially required for T cell receptor- compared to chemokine C-X-C receptor 4-mediated inside-out signaling pathways regulating T cell adhesion and migration in Jurkat T cells. PMID: 19812192

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  • 亞細(xì)胞定位：
    Cytoplasm.
  • 組織特異性：
    Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 6529

    OMIM: 601603

    KEGG: hsa:3937

    STRING: 9606.ENSP00000046794

    UniGene: Hs.304475