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  4. Phospho-RAD17 (S646) Antibody

Phospho-RAD17 (S646) Antibody

  • 中文名稱(chēng)：
    磷酸化-RAD17 (S646)兔多克隆抗體
  • 貨號(hào)：
    CSB-PA000755
  • 規(guī)格：
    ￥880
  • 圖片：
    • Western Blot analysis of K562, cells using Phospho-Rad17 (S646) Polyclonal Antibody
  • 其他：

產(chǎn)品詳情

  • Uniprot No.：
  • 基因名：
    RAD17
  • 別名：
    CCYC antibody; Cell cycle checkpoint protein antibody; Cell cycle checkpoint protein RAD17 antibody; FLJ41520 antibody; HRAD 17 antibody; hRad17 antibody; R24L antibody; Rad 17 antibody; Rad 24 antibody; RAD1 (S. pombe) homolog antibody; RAD1 homolog antibody; RAD17 antibody; RAD17 homolog (S. pombe) antibody; RAD17 homolog antibody; Rad17 like protein antibody; RAD17; S. pombe; homolog of antibody; RAD17_HUMAN antibody; RAD17Sp antibody; Rad24 antibody; Rad24; mouse; homolog of antibody; Rad24; S. cerevisiae; homolog of antibody; RF C activator 1 homolog antibody; RF C/activator 1 homolog antibody; RF-C/activator 1 homolog antibody
  • 宿主：
    Rabbit
  • 反應(yīng)種屬：
    Human,Mouse,Rat
  • 免疫原：
    Synthesized peptide derived from Human Rad17 around the phosphorylation site of S646.
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 抗體亞型：
    IgG
  • 純化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    WB, ELISA
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2000
    ELISA 1:10000
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the RAD1-RAD9-HUS1 complex and RHNO1 onto chromatin, and in CHEK1 activation. May also serve as a sensor of DNA replication progression, and may be involved in homologous recombination.
  • 基因功能參考文獻(xiàn)：
    1. DDX11 orchestrates jointly with 9-1-1 and its loader, RAD17, DNA damage tolerance at sites of bulky lesions, and endogenous abasic sites. These functions may explain the essential roles of DDX11 and its similarity with 9-1-1 during development. PMID: 30061412
    2. The Rad17 C-terminal tail is a molecular switch that regulates the 9-1-1 interaction and the ATR pathway. PMID: 28666868
    3. These data indicate that the interaction with the 9-1-1 complex is not required for Rad17 protein to be an efficient substrate for the UV-induced phosphorylation. Our data also raise the possibility that the 9-1-1 complex plays a negative regulatory role in the Rad17 phosphorylation. We also show that the nucleotide-binding activity of Rad17 is required for its nuclear localization. PMID: 27387238
    4. In a Japanese population, the variant allele of hRAD17 is significantly associated with a decreased risk of Colorectal Cancer among light smokers and rectal cancer patients and with an increased risk of Colorectal Cancer among heavy smokers. PMID: 28238011
    5. Authors show that BRCA1 and RAD17 genes, whose derived proteins play a pivotal role in DNA damage repair, are transcriptional targets of gain-of-function mutant p53 proteins. PMID: 25650659
    6. USP20 and Rad17 interact, and that this interaction is enhanced by UV exposure. We show that USP20 regulation of Rad17 is at the protein level in a proteasome-dependent manner. USP20 depletion results in poor activation of Chk1 protein by phosphorylation PMID: 24923443
    7. Rad17 is phosphorylated by ATM at Thr622 resulting in a direct interaction of Rad17 with NBS1, facilitating recruitment of MRE11, RAD50 and ATM to the DNA double-strand breaks. PMID: 24534091
    8. Our data suggest RAD17 as a novel target protein for gemcitabine combination therapy supplementing or complementing inhibition of CHK1. PMID: 23687379
    9. Knockdown of Rad17 with two independent siRNAs significantly reduced Chk1 phosphorylation and substantial RPA32 Ser33 phosphorylation. PMID: 23684611
    10. Data indicate that regulation of Rad17 turnover is through the Cdh1/anaphase-promoting complex pathway in breast cancer cells. PMID: 23637229
    11. Proteolysis of Rad17 by Cdh1/APC regulates checkpoint termination and recovery from genotoxic stress PMID: 20424596
    12. The four alternatively spliced forms differentially expressed in different tissues, in different phases of the cell cycle, and differentially responded to X-irradiation. PMID: 11602352
    13. upon replication block a Rad17/RF-C complex is recruited to sites of DNA lesions in late S phase, binds the Rad9/Hus1/Rad1 complex and enables it to interact with PCNA. An interaction of Rad17/RF-C with PCNA is mediated by the small RF-C p37 subunit PMID: 12400013
    14. Rad17 localizes to DNA replication sites and interacts with DNA polymerase epsilon. PMID: 14500819
    15. replication protein A (RPA) stimulates the binding of the Rad17-Rfc2-5 complex to single-stranded DNA PMID: 14605214
    16. we show a requirement for Rad17 and Hus1 to induce G(2) arrest as well as Vpr-induced phosphorylation of histone 2A variant X (H2AX) and formation of nuclear foci containing H2AX and breast cancer susceptibility protein 1 PMID: 15485898
    17. interacts with newly identified hMCM7 protein, a core component of the DNA replication apparatus PMID: 15538388
    18. Findings reveal a phosphorylation-dependent function of Rad17 in an ATR-Rad17-Claspin-Chk1-signaling cascade that responds to specific replication stress. PMID: 16885023
    19. Using siRNA to knock down Rad17 demonstrates that it is not essntial for the DNA replication checkpoint in Hela cells. PMID: 16951182
    20. Loss of hRAD17 expression occurs frequently in HNSCC, is often due to genomic deletion, and may facilitate genomic instability in HNSCC PMID: 17657792
    21. hRAD17 delayed growth of NIH3T3 fibroblasts transformed by the H-ras oncogene in nude mice. PMID: 18378394

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  • 亞細(xì)胞定位：
    Nucleus. Note=Phosphorylated form redistributes to discrete nuclear foci upon DNA damage.
  • 蛋白家族：
    Rad17/RAD24 family
  • 組織特異性：
    Overexpressed in various cancer cell lines and in colon carcinoma (at protein level). Isoform 2 and isoform 3 are the most abundant isoforms in non irradiated cells (at protein level). Ubiquitous at low levels. Highly expressed in testis, where it is expr
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 9807

    OMIM: 603139

    KEGG: hsa:5884

    STRING: 9606.ENSP00000370151

    UniGene: Hs.16184