RTN4RL2 Antibody
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中文名稱:RTN4RL2兔多克隆抗體
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貨號:CSB-PA774805NA01HU
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規(guī)格:¥440
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促銷:
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圖片:
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Western Blot
Positive WB detected in: K562 whole cell lysate, SH-SY5Y whole cell lysate, Hela whole cell lysate, HL-60 whole cell lysate
All lanes: RTN4RL2 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 47, 22 kDa
Observed band size: 47 kDa -
IHC image of CSB-PA774805NA01HU diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunofluorescence staining of MCF-7 cells with CSB-PA774805NA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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其他:
產(chǎn)品詳情
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產(chǎn)品名稱:Rabbit anti-Homo sapiens (Human) RTN4RL2 Polyclonal antibody
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Uniprot No.:
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基因名:RTN4RL2
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別名:RTN4RL2 antibody; NGRH1 antibody; NGRL3 antibody; Reticulon-4 receptor-like 2 antibody; Nogo receptor-like 3 antibody; Nogo-66 receptor homolog 1 antibody; Nogo-66 receptor-related protein 2 antibody; NgR2 antibody
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宿主:Rabbit
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反應(yīng)種屬:Human
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免疫原:Peptide sequence from Human Reticulon-4 receptor-like 2 protein (355-372AA)
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免疫原種屬:Homo sapiens (Human)
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標(biāo)記方式:Non-conjugated
本頁面中的產(chǎn)品,RTN4RL2 Antibody (CSB-PA774805NA01HU),的標(biāo)記方式是Non-conjugated。對于RTN4RL2 Antibody,我們還提供其他標(biāo)記。見下表:
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克隆類型:Polyclonal
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抗體亞型:IgG
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純化方式:Antigen Affinity Purified
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濃度:It differs from different batches. Please contact us to confirm it.
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保存緩沖液:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 -
產(chǎn)品提供形式:Liquid
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應(yīng)用范圍:ELISA, WB, IHC, IF
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推薦稀釋比:
Application Recommended Dilution WB 1:1000-1:5000 IHC 1:20-1:200 IF 1:50-1:200 -
Protocols:
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儲存條件:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期:Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能:Cell surface receptor that plays a functionally redundant role in the inhibition of neurite outgrowth mediated by MAG. Plays a functionally redundant role in postnatal brain development. Contributes to normal axon migration across the brain midline and normal formation of the corpus callosum. Does not seem to play a significant role in regulating axon regeneration in the adult central nervous system. Protects motoneurons against apoptosis; protection against apoptosis is probably mediated by MAG. Like other family members, plays a role in restricting the number dendritic spines and the number of synapses that are formed during brain development. Signaling mediates activation of Rho and downstream reorganization of the actin cytoskeleton.
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基因功能參考文獻(xiàn):
- Authors highlight the structural and biochemical aspects of the interaction of Nogo receptors (R1 and R2) with myelin inhibitors such as MAG, Nogo A and OMgp.[Review] PMID: 24956133
- These results indicate that a lack of NgR1/2 expression promotes the adhesion of DCs to myelin. PMID: 21906273
- predominantly expressed in the neurons of the embryonic and adult nervous systems PMID: 14664809
- The release of N-terminal fragment-NgRH1 was blocked by a hydroxamate-based zinc metalloproteinase inhibitor and tissue inhibitor of metalloproteinases-2 and -3 PMID: 15629437
- Nogo-66 receptor homolog 1 signaling regulates activity-dependent synaptic strength and neuronal plasticity. PMID: 18337405
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亞細(xì)胞定位:Cell membrane; Lipid-anchor, GPI-anchor. Membrane raft. Cell projection, dendrite. Perikaryon. Cell projection, axon.
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蛋白家族:Nogo receptor family
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組織特異性:Highly expressed in brain and liver. Expressed at lower levels in kidney, mammary gland, placenta, skeletal muscle, spleen and thyroid.
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