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CAPN2 Recombinant Monoclonal Antibody

  • 中文名稱：
    CAPN2重組抗體
  • 貨號(hào)：
    CSB-RA899086A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: U87 whole cell lysate, U251 whole cell lysate, PC3 whole cell lysate, A549 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate
      All lanes: CAPN2 antibody at 1:500
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 80, 72 kDa
      Observed band size: 72-95 kDa
  • 其他：

產(chǎn)品詳情

  • Uniprot No.：
  • 基因名：
  • 別名：
    Calpain-2 catalytic subunit (EC 3.4.22.53) (Calcium-activated neutral proteinase 2) (CANP 2) (Calpain M-type) (Calpain large polypeptide L2) (Calpain-2 large subunit) (Millimolar-calpain) (M-calpain), CAPN2, CANPL2
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from human CAPN2
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號(hào)：
    17E9
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2000
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226'. Proteolytically cleaves CPEB3 following neuronal stimulation which abolishes CPEB3 translational repressor activity, leading to translation of CPEB3 target mRNAs.
  • 基因功能參考文獻(xiàn)：
    1. High CCNA2 expression is associated with gefitinib resistance in lung cancer. PMID: 30106446
    2. in the field of neural injury, calpain-2 and caspase-3 proteases generate breakdown-products (BDPs) fragments that are indicative of different neural cell death mechanisms in different injury scenarios. Advanced proteomic techniques have shown a remarkable progress in identifying these BDPs experimentally PMID: 28112201
    3. The results of the present study suggested that miR93 regulated MMP1 and collagen I expression in fibroblasts via calpain2. miR93 mediated collagen expression in stress urinary incontinence via calpain2. PMID: 29115452
    4. Hyperactivated m-calpain induced cytoplasmic accumulation of truncated topoisomerase IIalpha in doxorubicin resistant T47D cells. PMID: 29425806
    5. Results find that overexpression of hTau increases intracellular calcium, which in turn activates calpain-2 and induces degradation of alpha4 nAChR. PMID: 27277673
    6. m-calpain translocated as the result of calcium influx was involved in DNA double-strand breaks repair, especially in the non-homologous end-joining pathway through proteolysis of nuclear Ku80. Cleaved Ku80 was still able to form a heterodimer with Ku70 and enhance DNA repair activity. PMID: 27121057
    7. 3 novel loci associated with serum alpha-carotene concentrations among a population that consumed a controlled diet. While replication is necessary, the CAPN2/CAPN8 locus provides compelling evidence for an association with serum alpha-carotene concentrations and may suggest a relationship with the development and progression of cancers. PMID: 28002826
    8. The inhibitory effects of BMP4 on PDGF-induced cell proliferation, collagen synthesis, and calpain-2 activation are impaired in pulmonary artery smooth muscle cells from pulmonary arterial hypertension patient. PMID: 28235949
    9. Calpain-2 upregulates PDGF-induced Akt phosphorylation in pulmonary vascular remodeling via an intracrine TGF-beta1/mTORC2 mechanism. PMID: 27099352
    10. the expression of CAPN2 was elevated in prostate cancer tissues than that in normal control tissues. PMID: 28280729
    11. Calpain 2-mediated cleavage of Atg3 and Atg7 accounts for the impaired autophagy. PMID: 27077802
    12. Calcineurin together with its upstream molecule, calpain 2, and its downstream effector, NFAT-c3, might contribute to the development of atrial fibrillation in patients with heart valve disease and diabetes. PMID: 27123462
    13. Calpain-2 modulates pulmonary vascular remodeling in pulmonary arterial hypertension. PMID: 26248159
    14. Taken together, our results demonstrated the deregulation of GAS2 in both AML and ALL and the requirement of GAS2-Calpain2 axis for the growth of leukemic cells. PMID: 26358320
    15. The combination of Ca2+ and ionomycin was required to activate calpain 2, resulting in the further degradation of Dicer and the appearance of a degradation product similar to that detected in platelets from some diabetic individuals. PMID: 25944670
    16. In a transgenic mouse model, hypoxia-triggered m-calpain activation is involved in endoplasmic reticulum stress-mediated Alzheimer's disease pathogenesis. PMID: 23889979
    17. Small interfering RNA (siRNA)-mediated silencing of m-calpain expression significantly suppressed the adhesive, migrative and invasive potentials of human hepatoma cells. PMID: 23733271
    18. The data reveal that hGAAP is a novel regulator of focal adhesion dynamics, cell adhesion, and migration by controlling localized Ca(2+)-dependent activation of calpain 2. PMID: 23940116
    19. The carboxyl tail of alpha-actinin-4 regulates its susceptibility to m-calpain and thus functions in cell migration and spreading. PMID: 23466492
    20. data demonstrate that GSK-3beta plays an important role in regulating tRXRalpha production by calpain II in cancer cells PMID: 23389291
    21. alpha-II spectrin was drastically accumulated in infected T cells derived from adult T-cell leukemia patients, whereas its digestive protease calpain-2 (CAN2) was significantly downregulated. PMID: 23538341
    22. The results suggest that calpain-2 and calpastatin expression is important in pancreatic cancers, influencing disease progression PMID: 23140395
    23. High expression of calpain-2 is significantly associated with resistance to platinum-based adjuvant chemotherapy. PMID: 22435971
    24. Expression of the large catalytic subunit of calpain-2 is significantly associated with clinical outcome of patients with triple-negative and basal-like disease. PMID: 22745213
    25. betaig-h3 co-localized with integrin alpha5beta1 to enhance the invasion of U87 cells, and that calpain-2, is involved in this process, acting as a downstream molecule. PMID: 22629380
    26. Two human hepatocellular carcinoma cell lines were researched using proteomics. A proteolysis network was built up, of which the CAPN2 centered subnetwork, including SPTBN1, ATP5B, and VIM, was more active in the highly metastatic HCC cell line. PMID: 22623320
    27. cleavage of CPEB3 by NMDA-activated calpain 2 accounts for the activity-related translation of CPEB3-targeted RNAs PMID: 22711986
    28. Staining intensity of calpain 2 in ovarian carcinoma decreased with increasing lymph node status. PMID: 22335024
    29. Subtype-selective induction of m-calpain in aorta during atherosclerotic progression is associated with proteolytic disorganization of VE-cadherin and proatherogenic hyperpermeability in cells. PMID: 22064597
    30. The expression of m-calpain-induced degradation products of extracellular matrix was correlated with the degree of disc degeneration in human intervertebral disc tissue PMID: 21839844
    31. Data show that transfection of cells with GRP78 or calpain I and calpain II siRNA reduced MPTB-mediated cell apoptosis in chondrosarcoma JJ012 cells. PMID: 21374734
    32. Calpain 2-mediated hTOP1 proteolysis not only impacts its biochemical activities but also alters some cellular functions of hTOP1. PMID: 21086148
    33. Confocal immunostaining demonstrated colocalization of m-calpain and the aggrecan product within the lower hypertrophic chondrocytes and in limited region of the pericellular matrix. PMID: 21117903
    34. NHE1 might participate in HIF-1-induced angiogenesis due, at least in part, to the alteration of calpain activity. PMID: 21185840
    35. Findings support the idea that calpain is involved in the turnover of LFA-1 adhesions at the rear of the cell. PMID: 21152086
    36. In brain, calpain-2 plays critical roles in developmental and adult synaptic plasticity. PMID: 20924799
    37. Knockdown of calpain 2 expression using shRNA or chemical inhibition of calpain activity reduced glioblastoma cell invasion by 90%. PMID: 20730561
    38. m-Calpain activation is regulated by its membrane localization and by its binding to phosphatidylinositol 4,5-bisphosphate. PMID: 20729206
    39. Data indicate that calpains are involved in the C-terminal truncation of aggrecan and might have a minor role in arthritic diseases. PMID: 20618160
    40. High m-calpain expression is associated with rhabdomyosarcoma aggressiveness. PMID: 20193680
    41. Overexpression of calpain-2 and low expression of calpastatin may involve in the pathological development of stress urinary incontinence. PMID: 19756344
    42. analysis of a novel role for calpain proteolysis of FAK in regulating adhesion dynamics in motile cells PMID: 20150423
    43. excessive TRPM7 channel activity causes oxidative and nitrosative stresses, producing cell rounding mediated by p38 MAPK/JNK-dependent activation of m-calpain PMID: 20070945
    44. m-Calpain antagonizes RhoA overactivation and endothelial barrier dysfunction under disturbed shear conditions. PMID: 19752040
    45. a higher calpain/calpastatin ratio collaborates with activated ERK to promote the generation of the low molecular weight-AR PMID: 19946123
    46. Overactivation of Ca(2+)-calpain pathways contributes to beta cell dysfunction and apoptosis in type 2 diabetes. PMID: 19861418
    47. Calpain activation in neurodegenerative diseases PMID: 12070670
    48. colocalization with detergent-insoluble rafts on T-cells PMID: 12150984
    49. localization of m-calpain within caveolae may contribute to maintenance of the enzyme in an inactive state and that m-calpain may also contribute to the regulation of calcium-sensing receptor levels. PMID: 12783889
    50. activation of m-calpain during mitosis is required for cells to establish the chromosome alignment by regulating some molecules that generate polar ejection force PMID: 14688278

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  • 亞細(xì)胞定位：
    Cytoplasm. Cell membrane. Note=Translocates to the plasma membrane upon Ca(2+) binding.
  • 蛋白家族：
    Peptidase C2 family
  • 組織特異性：
    Ubiquitous.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 1479

    OMIM: 114230

    KEGG: hsa:824

    STRING: 9606.ENSP00000295006

    UniGene: Hs.350899