NEUROD1 Recombinant Monoclonal Antibody
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中文名稱:NEUROD1重組抗體
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貨號:CSB-RA982003A0HU
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規(guī)格:¥1320
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圖片:
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Immunofluorescence staining of A549 with CSB-RA982003A0HU at 1:60, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 522-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA982003A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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其他:
產(chǎn)品詳情
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Uniprot No.:
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基因名:
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別名:Neurogenic differentiation factor 1 (NeuroD) (NeuroD1) (Class A basic helix-loop-helix protein 3) (bHLHa3), NEUROD1, BHLHA3 NEUROD
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反應(yīng)種屬:Human
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免疫原:A synthesized peptide derived from Human NEUROD1
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免疫原種屬:Homo sapiens (Human)
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標(biāo)記方式:Non-conjugated
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克隆類型:Monoclonal
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抗體亞型:Rabbit IgG
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純化方式:Affinity-chromatography
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克隆號:5D2
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濃度:It differs from different batches. Please contact us to confirm it.
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保存緩沖液:Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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產(chǎn)品提供形式:Liquid
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應(yīng)用范圍:ELISA, IF, FC
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推薦稀釋比:
Application Recommended Dilution IF 1:50-1:200 FC 1:50-1:200 -
Protocols:
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儲存條件:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期:Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能:Acts as a transcriptional activator: mediates transcriptional activation by binding to E box-containing promoter consensus core sequences 5'-CANNTG-3'. Associates with the p300/CBP transcription coactivator complex to stimulate transcription of the secretin gene as well as the gene encoding the cyclin-dependent kinase inhibitor CDKN1A. Contributes to the regulation of several cell differentiation pathways, like those that promote the formation of early retinal ganglion cells, inner ear sensory neurons, granule cells forming either the cerebellum or the dentate gyrus cell layer of the hippocampus, endocrine islet cells of the pancreas and enteroendocrine cells of the small intestine. Together with PAX6 or SIX3, is required for the regulation of amacrine cell fate specification. Also required for dendrite morphogenesis and maintenance in the cerebellar cortex. Associates with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis.
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基因功能參考文獻(xiàn):
- Data suggest that the first cases of MODY6 identified in Japan are result of missense (p.L157R, c.470T>G) or frameshift mutations (p.H206PfsTer38, c.616_617insC; p.P245RfsTer17, c.734delC; p.H206TfsTer56, c.616delC) in NEUROD1. The probands and affected family members exhibit intrinsically lower capacity of insulin secretion and neurological disorders. [CASE REPORT] PMID: 28664602
- Data suggest the EGR1-miR-30a-5p-NEUROD1 axis might serve as a promising biomarker for diagnosis and treatment monitoring for schizophrenic patients in acute psychotic state. EGR1 and miR-30a-5p were remarkably downregulated, whereas NEUROD1 was significantly upregulated in PBMNCs from patients in acute psychotic state. PMID: 28072411
- Mutation in NEUROD1 gene is associated with Maturity Onset Diabetes of the Young. PMID: 28095440
- the importance of the oligomeric state of CtBP for coactivation of NeuroD1-dependent transcription, was investigated. PMID: 27880001
- NeuroD1 seemed not sufficient to induce and maintain neuronal differentiation. Induction of neuronal differentiation by overexpression of Neurog1 initiated important steps for the development of glutamatergic neurons such as the spiral ganglion neurons PMID: 27423984
- detected c.133A > G (p. Ala45Thr) in children with sensorineural hearing loss PMID: 26634621
- Study reports a family with autosomal dominant diabetes related to a new NEUROD1 mutation, one of very few meeting Maturity Onset Diabetes of the Young criteria. PMID: 26773576
- RNAi of lentiviral vector target NeuroD can reduce the migration and invasion abilities of PANC-1 cells PMID: 24464628
- this study concludes that the novel mechanism would regulate the expression of ALK in neuroblastoma and that NeuroD1 should be significantly involved in neuroblastoma tumorigenesis. PMID: 25652313
- NEUROD1 is important for maintenance of the retina function and partial loss-of-function mutation in NEUROD1 is likely a rare cause of nonsyndromic ARRP. PMID: 25477324
- Increased expression of NeuroD1 subsequently leads to regulation of expression and function of the nicotinic acetylcholine receptor subunit cluster of alpha3, alpha5, and beta4. PMID: 24719457
- Transactivation of Ctbp was dependent on the histone H3 lysine 9 (H3K9) demethylase activity of LSD1 facilitates subsequent H3K9 acetylation by the NeuroD1-associated histone acetyltransferase, P300/CBP-associated factor. PMID: 24732800
- The variant A45T does not play a major role in the development of T2 Diabetes mellitus in East Asian descent. PMID: 23203005
- Gene expression profiling revealed that permissive lines are typified by lower expression of the early neurogenic transcription factor ASCL1 and, conversely, by higher expression of the late neurogenic transcription factor NEUROD1. PMID: 23739064
- NeuroD1 regulates survival and migration of neuroendocrine lung carcinomas via signaling molecules TrkB and NCAM. PMID: 23553831
- The overexpression of NeuroD may contribute to the tumorogenesis and development of pancreatic carcinoma, and is closely correlated to the cancer cell proliferation, p53 signal pathway and neural invasion. PMID: 22455846
- Combined transfection of the three transcriptional factors, PDX-1, NeuroD1, and MafA, causes differentiation of bone marrow mesenchymal stem cells into insulin-producing cells PMID: 22761608
- Photosensitive photoreceptor cells can be generated by combinations of transcription factors. The combination of CRX and RX generate immature photoreceptors: and additional NEUROD promotes maturation. PMID: 22558175
- Most, if not all, nasal chemosensory neurons derive from NeuroD1-expressing globose basal cells of the immediate neuronal precursor variety. PMID: 21800309
- Findings establish the critical role of the neuronal differentiation factor NeuroD1 in neuroblastoma as well as its functional relationship with the neuronal repellent factor Slit2. PMID: 21349947
- ATF2 interacts with beta-cell-enriched transcription factors, MafA, Pdx1, and beta2, and activates insulin gene transcription. PMID: 21278380
- NeuroD alone may not be sufficient to induce regulated insulin release in insulin-producing liver cells. PMID: 21084850
- Human NeuroD1 under control of the cytokeratin 19 promoter can induce differerentiation of pancreatic epithelial cells into insulin producing cells. PMID: 20692411
- syndrome resulting from homozygous loss of function mutations in NEUROD1 which is characterized by permanent neonatal diabetes. PMID: 20573748
- There was no association between methylation and expression in breast tumour specimens, with only 14% exhibiting NEUROD1 expression PMID: 19353266
- No significant association of NEUROD1 with retinopathy or nephropathy in Croatian patients with type I diabetes PMID: 20120526
- regulator of insulin transcription PMID: 11755474
- expression during trophoblast invasion PMID: 11900979
- Beta-cell dysfunction in late-onset diabetic subjects carrying homozygous mutation in transcription factor NeuroD1. PMID: 12200761
- The genetic polymorphism in NeuroD is associated with the development of early-onset type 2 diabetes. The presence of Thr45 allele may represent a risk factor for early-onset type 2 diabetes among Chinese. PMID: 12476420
- Polymorphism Ala45Thr is associated with Type 1 diabetes mellitus in Czech children PMID: 12639765
- Ala5Thr polymorphism of NeuroD1 plays a role in the risk of NIDDM in the examined Polish population PMID: 12861411
- NeuroD1/E47 transcription factors up-regulate IA-1 gene expression through the proximal E-box element of the IA-1 promoter PMID: 12890672
- Ala45 variant of BETA2/NeuroD1 may be associated with IDDM in Caucasians. PMID: 12951629
- NeuroD1 is differentially expressed in pituitary adenomas and its possible ontogenetic and/or pathogenetic implications in non-corticotroph tumors are discussed. PMID: 14759067
- No evidence of Ala(45)Thr polymorphism of the NeueroD gene and type 1 diabetes. PMID: 15047635
- Focus of this review is on recent progress in understanding the important role of BETA2/NeuroD1 in initiating neuronal differentiation and maintaining the nervous system. PMID: 15247487
- Polymorphism contributes to glucose intolerance in a South Indian population. PMID: 15277395
- NeuroD controls both common and distinct sets of molecules involved in cell survival and differentiation in different tissue types [review] PMID: 15650322
- co-expression and functional synergism of these beta-cell enriched transactivators, MafA, Pdx1, and Beta2, are critical for establishing the beta-cell-specific and efficient expression of the insulin gene. PMID: 15993959
- the SREBP-1c.BETA2.E47 complex is in a DNA looping structure which is required for efficient recruitment of CREB-binding protein/p300 PMID: 16055439
- we demonstrated that ISL1 and BETA2 could activate insulin gene transcription synergistically. PMID: 16321656
- Gender-specific association of the Ala45Thr variant of NEUROD1 with Type 1 diabetes in Brazilian women. PMID: 16357810
- results presented in this study define INSM1 as a transcriptional repressor of the neuroD/b2 gene. The molecular mechanism of INSM1 transcriptional repression is attributed to the recruitment of cyclin D1 and HDAC-1 and -3 PMID: 16569215
- The NeuroD1-Ala45Thr variation may itself have an important role in susceptibility to or be in disequilibrium with early-onset T2DM in Chinese. The Ala45Thr may affect the onset pattern of T2DM, i.e., early-onset but not late-onset T2DM in Chinese. PMID: 16773428
- helix-loop-helix (HLH) domain of basic helix-loop-helix (bHLH) family proteins such as NeuroD facilitate protein transduction into various cell lines. PMID: 16870135
- Expression of NeuroD1 versus chromogranin-A is more frequent in pCA, and correlates to increased indicators of malignancy in moderately to poorly differentiated pCA. PMID: 17126478
- These results suggest that NeuroD plays an important role in regulated exocytosis by inducing expressions of various components required in the process. PMID: 17217914
- A study evaluating the extent to which common variation in the six known maturity-onset diabetes of the young (MODY) genes, which cause a monogenic form of type 2 diabetes, is associated with type 2 diabetes is presented. PMID: 17327436
- Mutation in the NeuroD1/BETA2 gene contributes to the development of diabetes PMID: 17440689
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相關(guān)疾?。?/div>Maturity-onset diabetes of the young 6 (MODY6); Diabetes mellitus, non-insulin-dependent (NIDDM)亞細(xì)胞定位:Cytoplasm. Nucleus.數(shù)據(jù)庫鏈接:
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