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  4. ADORA1 Recombinant Monoclonal Antibody

ADORA1 Recombinant Monoclonal Antibody

  • 中文名稱：
    ADORA1重組抗體
  • 貨號：
    CSB-RA224168A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: A549 whole cell lysate, 293 whole cell lysate, U87 whole cell lysate
      All lanes: ADORA1 antibody at 1:2000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 37, 14 kDa
      Observed band size: 37 kDa
    • IHC image of CSB-RA224168A0HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    The product CSB-RA224168A0HU is a recombinant ADORA1 monoclonal antibody. It was generated by transfecting the human ADORA1 monoclonal antibody gene-vector clones into the cell line for in vitro production and subsequent purification from the tissue culture supernatant (TCS) through affinity-chromatography. This ADORA1 antibody is a rabbit IgG and can react with human ADORA1 protein. And it has been tested for use in ELISA, WB, and IHC applications.

    ADORA1 is an adenosine receptor that plays a key role in the immunomodulation of cancers. There is mounting evidence that ADORA1 overexpression that is dysregulated can induce a variety of tumors. ADORA1 expression was found to be substantially linked with tumor-infiltrating cells and immune biomarkers in thyroid papillary carcinoma (PTC) by Xu Lin et al., suggesting that it can be used as a diagnostic and prognostic biomarker for PTC.

  • Uniprot No.：
  • 基因名：
  • 別名：
    ADORA1; Adenosine receptor A1
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from human ADORA1
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    10D12
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IHC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
  • 基因功能參考文獻(xiàn)：
    1. ADORA1 is not a common risk factor or causal gene for Parkinson's disease or dementia with Lewy bodies in the European population PMID: 27987235
    2. monocyte-derived macrophages from ankylosing spondylitis patients expressed increased levels of A2AAR and reduced levels of A1 and A2BAR compared to healthy controls PMID: 29524036
    3. studies revealed individual trait characteristics for being either vulnerable or resilient to both alcohol and to sleep deprivation. Both interventions induce gradual increases in cerebral A1AR availability, pointing to a potential common molecular response mechanism. PMID: 30012607
    4. Data suggested that adenosine A1 receptor might potentiate glycinergic transmission through Galphai/PKA/alpha3 and Gbetagamma/alpha1ins pathways in inflamed rat. PMID: 28882563
    5. Elevated A1 adenosine receptor is associated with sleep deprivation. PMID: 28373571
    6. Mutation in ADORA1 may be associated with early-onset parkinsonism and cognitive dysfunction. PMID: 27134041
    7. Inhibition of cholinergic neurotransmission by beta3-adrenoceptors results from adenosine release via equilibrative nucleoside transporters and prejunctional A1-receptor stimulation in urinary bladder. PMID: 28446460
    8. This study showed that increased neuronal A1R expression in Rasmussen Encephalitis may be involved in prevention of seizures spread and seizures-induced damage, limitation of both seizures and inflammation atrophy in 1 cerebral hemisphere. PMID: 28789481
    9. A1R signaling enhances A2AR-mediated neurodegeneration [review] PMID: 28441750
    10. Mutational and computational analysis of A1-AR revealed a distinct conformation of the second extracellular loop and a wider extracellular cavity with a secondary binding pocket that can accommodate orthosteric and allosteric ligands. PMID: 28235198
    11. second extracellular loop is a key allosteric ligand-binding determinant. PMID: 27683013
    12. this study highlights a key role for extracellular loop 2 in A1AR orthosteric ligand binding and receptor activation. PMID: 27683014
    13. Dysregulation in ADORA1/ADORA2A expression was associated with glioma development. PMID: 27038930
    14. A1R mRNA levels and A1R density in PBMCs from idiopathic normal-pressure hydrocephalus patients were significantly lower than control subjects PMID: 26810542
    15. Its central activation could be and approach to achieving a shifted homeostasis in which physiology is downregulated to a homeostatically-regulated, stable hypometabolic state. (review) PMID: 27333659
    16. In postmortem human prefrontal cortex, adenosine A1 receptor is coupled preferentially, if not exclusively, to Galphai-3. PMID: 26213104
    17. Results show that ADORA1 rs2228079 and ADORA2A rs5751876 polymorphisms are associated with the risk of Gilles de la Tourette Syndrome, co-morbid disorders, and may affect the age of tics onset in Polish population. PMID: 26317759
    18. It has the intrinsic ability to form a heteromeric complex with metabotropic glutamate receptor type 1 and mutually modulate signaling. PMID: 26154847
    19. Paeoniflorin promotes the survival of cultured cortical neurons by increasing Akt and ERK1/2 phosphorylation via A1R-mediated transactivation of EGFR. PMID: 25661317
    20. Adenosine A1 receptor activation increases ENT1 activity via protein kinase C. PMID: 25725289
    21. This study revealed variation of regional A1AR availability associated with ADORA1 and particularly ADORA2A SNPs, several of which had previously been shown to be associated with increased anxiety and panic disorder. PMID: 24943643
    22. Stimulation of A1AR and A2BAR had a prominent anti-proliferative/pro-apoptotic effect on the glioblastoma stem cells. PMID: 25429616
    23. Results document that endogenously formed adenosine can chaperone its cognate A1 receptor. PMID: 25354767
    24. Results suggest that the anti-cancer effect of Omega-3 polyunsaturated fatty acids (Omega-3 PUFAs) on gastric cancer is at least partly dependent on activating the ADORA1-mediated apoptosis pathway. PMID: 24896321
    25. regulation of GABA uptake occurs via modulation of the GABA transporters by the adenosine A1 (A1R) and A2A (A2AR) receptors PMID: 23657626
    26. Induction of renal proximal tubular IL-11 is a critical intermediary in A1 adenosine receptor-mediated renal protection against acute ischemic kidney injury. PMID: 23813214
    27. Adenosine regulates bone metabolism via A1, A2A, and A2B receptors. PMID: 23682121
    28. Imiquimod suppresses propagation of herpes simplex virus 1 by upregulation of cystatin A via the adenosine receptor A1 pathway PMID: 22787201
    29. A1/A2 adenosine receptor activation does not directly contribute to cutaneous active vasodilatation. PMID: 22356216
    30. A(1) and A(2B)ARs could be potential biomarkers able to provide an early indication of SA risk and their stimulation may turn out to improve fetoplacental perfusion by increasing vascular endothelial growth factor (VEGF) and nitric oxide (NO). PMID: 22867902
    31. In patients with stable angina ADORA1 agonist capadenoson lowers exercise heart at comparable maximum workload, which is associated with improved total exercise time and prolongation of time to ischaemia. PMID: 22370739
    32. Results indicate a role for adenosine receptor A1 and A2A gene polymorphisms in susceptibility to apnoea of prematurity and bronchopulmonary dysplasia and in interindividual variability to caffeine response. PMID: 22462821
    33. Data suggest that expression of ADORA1 is too low to play a major role in regulation of lipogenic gene expression in hepatocytes; studies include investigation of gene expression under influence of ADORA1 agonists. PMID: 22449383
    34. data show that expression of A1Rs induced adipocyte differentiation, whereas A2bR expression inhibited adipogenesis and stimulated an osteoblastic phenotype PMID: 21730968
    35. AMP and a non-hydrolyzable AMP analog (deoxyadenosine 5'-monophosphonate, ACP) directly activated the adenosine A(1) receptor PMID: 22215671
    36. Inhibition of human mast cell activation would be a mechanism for A AR antagonists, but not A(2B) AR antagonists. PMID: 21506953
    37. Adenosine receptor expression and activation during the differentiation of mesenchymal stem cell to osteoblasts and adipocytes, was investigated. PMID: 21590734
    38. two genes involved in cardiovascular diseases, ADORA1 and PTGDS, were differentially up-regulated in epicardial adipose tissue compared to mediastinal and subcutaneous adipose tissue PMID: 21603615
    39. Data suggest that adenosine deficiency may be relevant to human epilepsy and that a ketogenic diet can reduce seizures by increasing adenosine A1 receptor-mediated inhibition. PMID: 21701065
    40. ADORA1 was expressed in nucleus, perinucleus and cytoplasm of retinal pigment epithelium. PMID: 21542986
    41. report implicating genetic variability in the A1AR with post-traumatic seizures. PMID: 20609566
    42. LUF5834 represents a novel high affinity radioligand for the A(1)R and may prove a useful tool to provide estimates of inverse agonist efficacy at this receptor. PMID: 20599769
    43. results suggest that adenosine-mediated signaling in the heart requires a balance between A(1)- and A(2A)-receptors PMID: 20354569
    44. Report fluorescence-based techniques, and in particular the development of fluorescent ligands, so study pharmacology of the adenosine A1 receptor. PMID: 20105183
    45. Findings suggest that the adenosine A1 (and possibly coordinated with A2a) receptors contribute to dendritic cell differentiation and survival. PMID: 20085598
    46. Studies indicate that adenosine mediates its actions by means of activation of specifiic G protein-coupled receptors, for which 4 subbtypes: A1R, A2AR, A2BR and A3R have been identified so far. PMID: 19883624
    47. Adenosine, thus, suppresses CW2 human colonic cancer growth by inducing apoptosis as mediated via A(1) adenosine receptors. PMID: 19822392
    48. Data suggest that adenosine receptor modulation may be useful for refining the use of chemotherapeutic drugs to treat human cancer more effectively. PMID: 19794965
    49. ADORA1 polymorphisms may represent good candidate markers for schizophrenia research and ADORA1 may be involved in the pathophysiological mechanisms of schizophrenia in Japanese populations PMID: 19820430
    50. Human A1 adenosine receptor overexpressed in adipose tissue of transgenic mice protects them from obesity-related insulin resistance. PMID: 11703426

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  • 亞細(xì)胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    G-protein coupled receptor 1 family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 262

    OMIM: 102775

    KEGG: hsa:134

    STRING: 9606.ENSP00000308549

    UniGene: Hs.77867