BAK1 Recombinant Monoclonal Antibody
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中文名稱(chēng):BAK1重組抗體
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貨號(hào):CSB-RA624111A0HU
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規(guī)格:¥1320
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圖片:
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Western Blot
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, THP-1 whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, K562 whole cell lysate, Colo320 whole cell lysate
All lanes: BAK1 antibody at 0.9μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 24, 17 KDa
Observed band size: 24 KDa -
IHC image of CSB-RA624111A0HU diluted at 1:90 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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IHC image of CSB-RA624111A0HU diluted at 1:90 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunoprecipitating BAK1 in HEK293 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-RA624111A0HU in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-RA624111A0HU (3μg) + HEK293 whole cell lysate (500μg)
Lane 3: HEK293 whole cell lysate (20μg) -
Overlay histogram showing Hela cells stained with CSB-RA624111A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:
The recombinant BAK1 antibody is a monoclonal antibody made in vitro using the BAK1 antibody genes that are typically expressed from a plasmid in a stable mammalian cell line. The genes coding for the BAK1 antibody will ultimately assemble into a fully functional antibody after translation. The synthesized antibody is the recombinant antibody against BAK1. It underwent purification using affinity-chromatography. This recombinant BAK1 antibody is suitable for use in the ELISA, WB, IHC, FC, IP to detect the BAK1 protein from Human.
BAK1 is involved in the control of a number of different types of programmed cell death (PCD), including immunity and development- and defense-related PCD. It has been demonstrated that BAK1 serves as a primary player at the intersection of multiple physiological processes, including the regulation of development, and responses to biotic stresses. The involvement of BAK1 in brassinosteroid (BR) signaling, vascular differentiation, stem elongation, flowering, floral abscission, fertility, and senescence have also been documented.
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Uniprot No.:
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基因名:
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別名:BAK1; BAK; BCL2L7; CDN1; Bcl-2 homologous antagonist/killer; Apoptosis regulator BAK; Bcl-2-like protein 7; Bcl2-L-7
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反應(yīng)種屬:Human
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免疫原:A synthesized peptide derived from human BAK1
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免疫原種屬:Homo sapiens (Human)
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標(biāo)記方式:Non-conjugated
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克隆類(lèi)型:Monoclonal
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抗體亞型:Rabbit IgG
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純化方式:Affinity-chromatography
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克隆號(hào):8D1
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濃度:It differs from different batches. Please contact us to confirm it.
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保存緩沖液:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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產(chǎn)品提供形式:Liquid
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應(yīng)用范圍:ELISA, WB, IHC, FC, IP
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推薦稀釋比:
Application Recommended Dilution WB 1:500-1:5000 IHC 1:50-1:200 IP 1:200-1:1000 -
Protocols:
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儲(chǔ)存條件:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期:Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能:Plays a role in the mitochondrial apoptosic process. Upon arrival of cell death signals, promotes mitochondrial outer membrane (MOM) permeabilization by oligomerizing to form pores within the MOM. This releases apoptogenic factors into the cytosol, including cytochrome c, promoting the activation of caspase 9 which in turn processes and activates the effector caspases.
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基因功能參考文獻(xiàn):
- Our findings suggested that ZBP-89 and Sp1 overexpression induced Bak expression in a genetic manner. Increased Bak level was associated with poor patient survival, whereas high level of Sp1 is a beneficial factor for patient survival. PMID: 29653560
- Study reports the proximal alpha1-alpha2 loop as a second activation site in Bak and in mitochondrial Bax. PMID: 27217060
- The proapoptotic BCL-2 proteins BAX and BAK can commit a cell to its programmed death by permeabilizing the outer mitochondrial membrane and subsequent initiation of the caspase cascade. (Review) PMID: 28676391
- Data suggest that ECAD, STAT3, Bak, and Bcl-xL are expressed in affected endometrial tissues of women with endometrioid adenocarcinoma depending on neoplasm staging and cell differentiation. This study was conducted using immunohistochemistry of surgically resected tissues. (ECAD = E-cadherin; STAT3 = signal transducer and activator of transcription 3 protein; Bcl-xL = BCL2 associated agonist of cell death) PMID: 28937296
- This comprehensive survey showed that in each Bak dimer the N-termini are fully solvent-exposed and mobile, the core is highly structured, and the C-termini are flexible but restrained by their contact with the membrane. PMID: 28182867
- The BAK1/BCL2 ratio was positively correlated with the results of audiometric tests. Our results indicate that BAK-mediated apoptosis may be a core mechanism in the progression of age-related hearing impairment in humans PMID: 27555755
- UMI-77 enhances TRAIL-induced apoptosis by unsequestering Bim and Bak, which provides a novel therapeutic strategy for the treatment of gliomas. PMID: 28337703
- after the induction of apoptosis, Bak switches from its association with Mtx2 and VDAC2 to a closer association with Mtx1. PMID: 27845183
- Conversion of Bim-BH3 from Activator to Inhibitor of Bak through Structure-Based Design. PMID: 29149594
- The results strongly suggest that the direct apoptosis-activation activities of Bid, Bim, Puma, and p53 are not essential for activating Bax/Bak once the anti-apoptotic Bcl-2 proteins are neutralized. PMID: 27310874
- Studied BAK1, SPRY4 and GAB2 SNPs in pediatric germ cell tumors(GCT); found a variant in SPRY4 was associated with reduced risk of GCT; a variant in BAK1 was positively associated with GCT with a strong estimated effect for testis tumors; and a SNP in GAB2 was associated with increased risk for GCT. PMID: 28295819
- These results not only establish Bok as a Bak- and Bax-independent apoptosis inducer, but also suggest a potential impact of Bok expression in ovarian cancer therapy. PMID: 27076518
- The low-level constitutive production of platelets caused by the G allele of rs5745568 in BAK1 protein seems to increase the risk of bleeding in dengue infection PMID: 27401010
- the expression of Bak1 was downregulated in CRC tumor tissues and was reversely correlated with the expression of miR410, which provided further support that Bak1 was regulated by miR410. PMID: 27177325
- Low expression of BAX is associated with docetaxel resistance in gastric cancer. PMID: 26486506
- Polymorphisms of KITLG, SPRY4, and BAK1 genes in patients with testicular germ cell tumors and individuals with infertility associated with AZFc deletion of the Y chromosome PMID: 28064312
- Different selenium concentrations had varying effects on BAK1 and APAF1 levels. APAF1 may play an important role in the pathogenesis of KBD PMID: 26179084
- Findings argue for auto-activation of Bak in the absence of anti-apoptotic Bcl-2 proteins and provide evidence of profound differences in the activation of Bax and Bak. PMID: 26610208
- LTX-315 kills cancer cells through Bax/Bak-regulated mitochondrial membrane permeabilization PMID: 26378049
- E6 proteins of human papillomavirus types 6 and 11 are able to protect keratinocytes from apoptosis via Bak degradation. PMID: 26743580
- Bak apoptotic pores involve a flexible C-terminal region and juxtaposition of the C-terminal transmembrane domains. PMID: 25744027
- Low expression of pro-apoptotic Bak protein in in Waldenstrom macroglobulinemia. PMID: 25893290
- Report shows that high Bak expression is associated with the favorable overall survival rates of breast cancer patients. PMID: 26406239
- alpha1 dissociation is a key step in unfolding Bak into three major components, the N terminus, the core (alpha2-alpha5) and the latch (alpha6-alpha8) that is required for apoptosis. PMID: 25880232
- the BH3 mimetic ABT-737 induces autophagy through a BAX and BAK1-independent mechanism that likely involves disruption of BECN1 binding to antiapoptotic BCL2 family members PMID: 25715028
- Data with full-length proteins indicate that most BH3-only proteins can directly activate both Bak and Bax. PMID: 25906158
- Puma is the major mediator of virus-induced Bax/Bak activation and mitochondrial membrane permeabilization induction. PMID: 26030884
- Data show that mitochondrial X-linked inhibitor of apoptosis protein (XIAP) entry requires apoptosis regulatory proteins Bax or Bak through mitochondrial permeabilization and Smac/DIABLO protein degradation. PMID: 26134559
- BMX directly inhibits a core component of the intrinsic apoptosis machinery opens opportunities to improve the efficacy of existing chemotherapy by potentiating BAK-driven cell death in cancer cells. PMID: 25649765
- high BAK expression correlates with sensitivity of clinical acute myelogenous leukemia to chemotherapy, whereas low BAK levels correlate with resistance and relapse PMID: 26494789
- Motifs of VDAC2 required for mitochondrial Bak import and tBid-induced apoptosis. PMID: 26417093
- By down-regulating Bak, P53, P21, BRCA1, BCL-Xl. PMID: 25773855
- Results show that in thyroid tumours and cell lines, VDAC2 is upregulated and BAK1 downregulated. Also, transient knockdown of VDAC2 promoted upregulation of the BAK1 expression, and increased susceptibility to sorafenib treatment. PMID: 25617717
- Chk1 activity is required for BAK multimerization in association with PUMA during mitochondrial apoptosis PMID: 25012639
- Studies show that necrosis requires Bcl-2-associated protein x (Bax) and Bcl-2 homologues antagonist/killer (Bak), which can regulate the permeability characteristics of the outer mitochondrial membrane. PMID: 25999420
- G-Rh2 causes rapid and dramatic translocation of both Bak and Bax, which subsequently triggers mitochondrial cytochrome c release and consequent caspase activation. PMID: 23443079
- Data show that E3 ubiquitin-protein ligase HERC1 interacts with bcl-2 homologous antagonist-killer protein BAK in HPV5 E6-expressing cells that have been damaged by UV. PMID: 25408501
- Data show that proto-oncogene protein ERBB3 knockdown resulted in cell cycle arrest and activation of Bak and Bax-dependent apoptosis. PMID: 24970817
- The Bak and Bax genes expressions were analyzed in 23 breast cancer patients. PMID: 24672785
- The mitochondrial metalloprotease OMA1 was activated in a Bax- and Bak-dependent fashion. PMID: 25275009
- Helices contribute to mitochondrial outer membrane perforation in that oligomers Bak alpha5 (like Bax alpha5) remain part of the protein core and a membrane-impermeable cysteine reagent labels cysteines placed at many positions in alpha5 and alpha6 of both Bak and Bax. PMID: 25228770
- enhanced efficacy of combination of mdivi-1 and death ligands is not dependent on the originally reported target of mdivi-1, Drp1, and is also not dependent on the two important pro-apoptotic Bcl-2 family proteins Bax and Bak PMID: 25446129
- the reduction of Bax shuttling to the level of Bak retrotranslocation results in full Bax toxicity even in absence of apoptosis induction. PMID: 25378477
- Clitocine may activate Bak by disrupting the interaction between Mcl-1. PMID: 25304383
- Bak interacts with Mtx2 together with VDAC2 in resting cells and with Mtx1 upon induction of apoptosis. PMID: 24794530
- This progress has resolved longstanding controversies, but has also challenged established theories in the apoptosis field, it focus on the recent progress made in characterizing the proapoptotic Bax and Bak. PMID: 24162660
- Caspase 8 cleavage by HIV protease activates BAK, enabling HIV to kill infected T cells. PMID: 25246614
- Data shows that Bak undergoes a conformational change similar to that of Bax upon activation. PMID: 25175025
- our data strongly support an important role for miR-125b on conferring TMZ resistance through targeting Bak1 expression. PMID: 24643683
- Authors demonstrate that during vaccinia virus infection, engagement of Bim and Bak by F1L is crucial for subversion of host cell apoptosis. PMID: 24850748
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亞細(xì)胞定位:Mitochondrion outer membrane; Single-pass membrane protein.
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蛋白家族:Bcl-2 family
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組織特異性:Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle.
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