GATA3 Recombinant Monoclonal Antibody
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中文名稱:GATA3重組抗體
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貨號:CSB-RA196111A0HU
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規(guī)格:¥1320
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圖片:
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Western Blot
Positive WB detected in: RAW264.7 whole cell lysate
All lanes: GATA3 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 48, 49 kDa
Observed band size: 48 kDa -
IHC image of CSB-RA196111A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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IHC image of CSB-RA196111A0HU diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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其他:
產(chǎn)品詳情
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Uniprot No.:
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基因名:
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別名:Trans-acting T-cell-specific transcription factor GATA-3 (GATA-binding factor 3), GATA3
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反應(yīng)種屬:Human
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免疫原:A synthesized peptide derived from human GATA3
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免疫原種屬:Homo sapiens (Human)
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標(biāo)記方式:Non-conjugated
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克隆類型:Monoclonal
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抗體亞型:Rabbit IgG
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純化方式:Affinity-chromatography
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克隆號:2A9
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濃度:It differs from different batches. Please contact us to confirm it.
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保存緩沖液:Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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產(chǎn)品提供形式:Liquid
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應(yīng)用范圍:ELISA, WB, IHC
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推薦稀釋比:
Application Recommended Dilution WB 1:500-1:5000 IHC 1:50-1:200 -
Protocols:
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儲存條件:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期:Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
相關(guān)產(chǎn)品
靶點詳情
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功能:Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses. Positively regulates ASB2 expression.
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基因功能參考文獻:
- The data indicate that expression of CRTh2 is regulated through the competitive action of GATA3 and NFAT1. PMID: 29969451
- the present study postulates that the genetic variation of the transcription factor GATA3, not STAT4, is associated with the risk of type 2 diabetes in the Bangladeshi population. PMID: 30044774
- one allele of the GATA3 second zinc finger leads to loss of binding and decreased expression at a subset of genes, including Progesterone Receptor PMID: 29535312
- Study established an important role of p38gamma MAPK in epithelial-mesenchymal transition (EMT) and identified a novel signaling pathway for p38gamma MAPK-mediated tumor promotion. p38gamma MAPK regulated miR-200b through inhibiting GATA3 by inducing its ubiquitination, leading proteasome-dependent degradation. PMID: 30251680
- Treg cells from asthmatic patients expressed more FOXP3 as well as GATA3; the expression level of GATA3 negatively correlated with FEV1%pred. Increased expressions of USP21 and PIM2 in Treg cells from asthmatic patients were found. PMID: 30013989
- GATA 3 is more sensitive marker than mammaglobin and GCDFP-15 for diagnosing metastatic breast carcinoma in cytological cell block materials PMID: 29235613
- This study demonstrates consistent GATA-3 staining in walthard nests, rare staining in adenomatoid tumors, and is otherwise rarely positive in normal urologic and gynecologic mesothelia. Furthermore, GATA-3 is uniformly positive in epididymi and negative in efferent ductules which may reflect the embryological evolvement of these tissues. PMID: 28582342
- GATA3 staining to differentiate primary cutaneous apocrine cribriform carcinoma from skin breast cancer metastasis has a high negative predictive value PMID: 29431200
- GATA3 mutations, recently observed in breast cancer, encode active transcription factors. PMID: 30061207
- KMT1A positively regulated the self-renewal and tumorigenicity of human Bladder cancer stem cells via KMT1A-GATA3-STAT3 circuit, in which KMT1A could be a promising target for bladder cancer therapy. PMID: 28765327
- Defective sirtuin-1 was found to increase IL-4 expression through acetylation of GATA-3 in patients with severe asthma compared with healthy controls. PMID: 26627546
- GATA3 activation was diminished upon cultivation of T cells with RNase 7. PMID: 28378334
- overexpression of GATA3 and FOXA1 cooperate with PPAR activation to drive transdifferentiation of a basal bladder cancer cells to a luminial phenotype. PMID: 27924948
- The data have demonstrated that GATA3 is by far the most reliable breast-specific immunomarker in both surgical and cytological specimens PMID: 28965624
- GATA3 interacts with and is acetylated by the acetyltransferase CBP. The major acetylated site of GATA3 in lung adenocarcinoma cells is lysine 119. PMID: 29453984
- GATA3 may be useful as a part of a panel of immunohistochemical markers in narrowing the differential diagnosis between pheochromocytoma and adrenal cortical carcinoma PMID: 28374498
- Authors demonstrated that ER(alpha), GATA3 and FOXA1 form a transcriptional complex with Ell3 to regulate IL-20 expression in ER(+) breast cancer cells. FOXA1 represses IL-20 expression, whereas GATA3 and ER(alpha) activate it. PMID: 28514748
- We have identified ZPO2 as a negative regulator of GATA3, thus providing an alternative mechanism that results in a reduction in or perhaps even loss of GATA3 during breast cancer development. PMID: 28258171
- Our study has demonstrated that GATA3 expression is common in primary triple-negative breast carcinomas PMID: 28211079
- GATA3 is a sensitive marker for primary genital extramammary paget disease PMID: 28693610
- Benign prostate glands with radiation atypia show diffuse positivity for GATA3 PMID: 28316088
- In summary, we documented a transcriptional regulation of GATA3 in glioblastoma cells upon transfection with GAB sequence. PMID: 28614770
- Results showed that GATA3 along with TRPS1 are distinctively overexpressed in breast cancer (BC) among all GATA family members and predict better survival in patients with BC. GATA3 is then a distinctive biomarker and essential prognostic factor in BC. PMID: 28423734
- Authors found a high sensitivity for all the markers analyzed. Moreover, the expression of NY-BR-1 and GATA-3 seemed the most effective for labelling male breast cancer in primary and metastatic setting. PMID: 29116378
- These results suggest that high GATA3 expression is a predictor of a poor prognosis in peripheral T cell lymphoma, and that T lymphoma cells promote M2-type macrophage differentiation through a GATA3-dependent mechanism. PMID: 27589565
- We demonstrate that engagement of the T-cell receptor (TCR) on malignant T cells culminates in NF-kappaB activation and the upregulation of GATA-3 expression, both of which regulate the growth and survival of conventional T cells, and were shown to promote chemotherapy resistance in malignant T cells PMID: 27780854
- These results suggest that the ELK3-GATA3 axis is a major pathway that promotes metastasis of breast cancer MDA-MB-231 cells PMID: 27556500
- As a transcriptionally regulated program, urothelial differentiation operates as a heterarchy, wherein GATA3 is able to co-operate with FOXA1 to drive expression of luminal marker genes, but that P63 has potential to transrepress expression of the same genes. PMID: 28282036
- GATA-3 in lymphocytes showed the increased expression of GATA-3 in elderly people in comparison with the young. Women demonstrated higher expression of GATA-3 in compare with men. PMID: 28509479
- C-MYC is related to GATA3 and Ki-67 expression and is associated with poor prognosis in nodal peripheral T-cell lymphomas PMID: 27151990
- The GATA3 rs3824662 A allele and AA genotype may be risk factors for the development of pediatric ALL especially B-ALL in the studied cohort of Egyptian patients. The AA genotype is associated with shorter DSF, increased incidence of relapse and poor prognosis in pediatric ALL. PMID: 27684731
- Mutation in the GATA-3 gene is associated with hypoparathyroidism, deafness and renal dysplasia syndrome. PMID: 27387476
- GATA3 is a reliable diagnostic marker for Neuroblastomas not only in scant/limited surgical specimens but also in cytologic samples, including air-dried touch imprints, which have previously been undescribed for this marker. PMID: 28976719
- Dysregulation of JAM-A via p63/GATA-3 signaling pathway occurs in squamous cell carcinomas of the head and neck. PMID: 27036044
- FBXW7alpha inhibits breast cancer cells survival by promoting GATA3 degradation/destabilizing GATA3. PMID: 28722108
- GATA3-driven expression of miR-503 inhibits prostate cancer progression by repressing ZNF217 expression. PMID: 27267060
- MIR-720 suppresses M2 macrophage polarization by targeting GATA3; MIR-720 is down-regulated in breast cancer macrophages. PMID: 27354564
- the model of mutually antagonistic differentiation programs driven by mutually exclusively expressed T-bet or GATA-3 does not completely explain natural CD4 T cell priming outcomes PMID: 29088218
- GATA3 expression is associated with breast carcinomas of luminal subtype and low histological grade. PMID: 28428285
- The aim of this study was to investigate the clinical significance of three immune cell-related transcription factors, T-bet, GATA-3 and Bcl-6 in bladder cancer in Tunisian patients. PMID: 27237631
- Data delineates the regulatory mechanisms of GATA3 in DNA double-strand breaks repair and strongly suggests that it might act as a tumor suppressor by promoting CtIP expression and homologous recombination to stabilize genomes. PMID: 28481869
- data provide new insights into the role of SEMA3B in mammary gland and provides a new branch of GATA3 signaling that is pivotal for inhibition of breast cancer progression and metastasis PMID: 28581515
- It gene expression is up-regulated by EP300-ZNF384 fusion gene product. PMID: 28378055
- Coexpression of GATA3 and CK7 in most clear cell papillary renal cell carcinomas provides evidence of their origin from distal nephron and can be used in differential diagnosis. PMID: 28705707
- Our findings suggest that GATA3 stabilizes HIF-1alpha to enhance cancer invasiveness under hypoxia PMID: 28263977
- GATA-3 is a sensitive and specific marker for the diagnosis of acute leukemias with T-cell differentiation. PMID: 28551327
- In resected lung adenocarcinoma, high GATA3 expression is associated with poorer prognosis for both overall survival and disease free survival. PMID: 28322854
- Loss of GATA3 expression was found to be an independent predictor of poor patient outcomes in bladder urothelial carcinoma. PMID: 28428106
- Meta-analysis revealed that high expression of GATA3 in breast cancer is associated with improved time to tumor progression. PMID: 28394898
- Rs17144046 located near GATA3 was significantly associated with benign prostatic hyperplasia and lower urinary tract symptoms. PMID: 28656603
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相關(guān)疾?。?/div>Hypoparathyroidism, sensorineural deafness, and renal disease (HDR)亞細胞定位:Nucleus.組織特異性:T-cells and endothelial cells.數(shù)據(jù)庫鏈接:
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