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  4. Phospho-PXN (Y118) Recombinant Monoclonal Antibody

Phospho-PXN (Y118) Recombinant Monoclonal Antibody

  • 中文名稱：
    磷酸化-PXN (Y118)重組抗體
  • 貨號(hào)：
    CSB-RA019112A118phHU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in Hela whole cell lysate,A549 whole cell lysate,HepG2 whole cell lysate(treated with Calyculin A or EGF)
      All lanes Phospho-PXN antibody at 1.43μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 68 KDa
      Observed band size: 68 KDa
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    The vectors expressing anti-PXN antibody were constructed as follows: immunizing an animal with a synthesized peptide derived from human Phospho-PXN (Y118), isolating the positive splenocyte and extracting RNA, obtaining DNA by reverse transcription, sequencing and screening PXN antibody gene, and amplifying heavy and light chain sequence by PCR and cloning them into plasma vectors. After that, the vector clones were transfected into the mammalian cells for production. The product is the recombinant PXN antibody. Recombinant PXN antibody in the culture medium was purified using affinity-chromatography. It can react with PXN protein from Human and is used in the ELISA, WB.

    PXN encodes a cytoskeletal protein involved in the actin-membrane attachment at sites of cell adhesion to the extracellular matrix. According to some studies, PXN may have the following characteristics.
    The MBNL3 splicing factor increases the expression of PXN through alternative splicing of lncRNA-PXN-AS1 and promotes hepatocellular carcinoma. Nobiletin inhibits angiogenesis by modulating Src/FAK/STAT3-mediated signaling in ER+ breast cancer cells via PXN. ETV4 overexpression promotes the progression of non-small cell lung cancer by upregulating the transcription of PXN and MMP1. Pan-cancer analysis reveals immunological roles and prognostic potential of PXN in human cancers.

  • Uniprot No.：
  • 基因名：
  • 別名：
    FLJ16691 antibody; FLJ23042 antibody; Paired box protein Pax 1 antibody; PAX 1 antibody; PAX1 antibody; PAXI_HUMAN antibody; Paxillin alpha antibody; Paxillin antibody; PXN antibody; PXN protein antibody
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from Human Phospho-PXN (Y118)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號(hào)：
    1F11
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
  • 基因功能參考文獻(xiàn)：
    1. Data suggest that FGFR3 with mutation found in patients with SADDAN (but not FGFR3 with mutation found in patients with TDII) affects cytoskeleton organization in chondrocytes by inducing tyrosine hyperphosphorylation of paxillin. (FGFR3 = fibroblast growth factor receptor 3; SADDAN = Severe Achondroplasia with Developmental Delay and Acanthosis Nigricans; TDII = Thanatophoric Dysplasia type II) PMID: 29242050
    2. overexpression of Paxillin significantly decreased tumor volume of colorectal cancer; miR-24 was overexpressed in natural killer cells and inhibited paxillin expression. PMID: 29494963
    3. XIST positively regulated PXN levels by sponging miR-137 in vitro and in vivo. Collectively, our study provided the evidence for the cross-talk between XIST, miR-137, and PXN, shedding light on the therapy for non-small cell lung cancer . PMID: 29337100
    4. we herein demonstrated that frequent overexpression of PXN in cervical cancer was associated with advanced tumor stage, poor differentiation and metastasis, and poor predictive outcomes PMID: 29318915
    5. Kindlin supports platelet GPIIB IIIA activation by interacting with paxillin. PMID: 28954813
    6. we collected paraffin specimens from 85 GBM patients and evaluated the expression pattern of paxillin. Notably, we found that discernable paxillin signals were detected in 67 out of 85 samples. Given that leading edge is critical for cancer cell migration, we propose that NA treatment may be developed into a potential therapy for malignant glioma PMID: 28656206
    7. Pxn binding to the CD103 cytoplasmic tail triggers alphaEbeta7 integrin outside-in signaling that promotes CD8(+) T-cell migratory behavior and effector functions. PMID: 29021139
    8. REVIEW of the functions of paxillin in pathological conditions, particularly in cell migration PMID: 28214467
    9. This study showed that the PXN plays as an oncogene in glioma progression and suggests a new potential biotarget for therapy PMID: 27637748
    10. The MBNL3 splicing factor promotes hepatocellular carcinoma by increasing paxillin expression through the alternative splicing of lncRNA-PXN-AS1. PMID: 28553938
    11. These data suggest that paxillin appears to influence major cell functions in a diverse range of prostate and breast cancer models. The responsiveness of cells to environmental factors such as HGF or BME may be influenced by paxillin status, although this seems to be dependent on cell type PMID: 28739717
    12. The role of paxillin in the aging process of skin cells is reviewed. PMID: 27708212
    13. interactions between Cat-1 and its binding partner paxillin are necessary to ensure sufficient Akt activation so that cancer cells are able to grow under anchorage-independent conditions PMID: 28100775
    14. The present findings demonstrated that the anticancer effect of docetaxel induces the apoptosis of prostate cancer via the suppression of the cofilin1 and paxillin signaling pathways, which will assist in setting a stage for the clinical treatment of prostate cancer. PMID: 27035282
    15. Results showed that the positive rate of PXN was significantly higher in the colorectal adenocarcinoma samples and correlated with TNM stage, distant metastasis and recurrence additionally to cetuximab resistance. PMID: 26530439
    16. blockade of GD3-mediated growth signaling pathways by siRNAs might be a novel and promising therapeutic strategy against malignant melanomas, provided signaling molecules such as p130Cas and paxillin are significantly expressed in individual cases. PMID: 27068854
    17. These findings suggest that PXN expression has potential use as a novel biomarker of laryngeal squamous cell carcinoma patients and may serve as an independent predictive factor for prognosis. PMID: 26464671
    18. Mode of Action of Functionally Important Regions in the Intrinsically Disordered Paxillin PMID: 26928467
    19. Paxillin was expressed at significantly higher levels in colorectal cancer tissues and might serve as a potential prognostic indicator in patients with colorectal cancer PMID: 26159303
    20. these data suggested that miR-145 plays a pivotal role in colon cancer through inhibiting cell proliferation migration and invasion, and miR-145 may serve as a tumor suppressor by targeting paxillin gene. PMID: 25973017
    21. In colorectal cancers PXN was positively correlated with Bcl-2, pBcl-2-S87, and MMP2 expression. PXN promotes Bcl-2 phosphorylation at Serine 87 via ERK activation, increases xenograft tumor formation, and associates with poor patient outcome. PMID: 25826088
    22. Bcl-2 stabilization by paxillin confers 5-fluorouracil resistance in colorectal cancer. PMID: 25323586
    23. Fascin-1 and paxillin were expressed in 58% and 43% of infiltrating duct carcinoma cases. There was a significant correlation between fascin-1 and paxillin expression and tumor grade, clinical stage, lymph-node metastasis grade, and HER2 expression. PMID: 26349603
    24. Suggest paxillin up-regulation and phosphorylation as an important mechanism of vascular remodelling underlying pulmonary hypertension. PMID: 25231004
    25. During early cell spreading, DLC1 is preferentially localized at the inner/mature adhesions whereas phosphorylated paxillin occupies the outer/nascent focal adhesions. In addition, DLC1 downregulates paxillin turnover PMID: 25448629
    26. The expression levels of Wnt5a, p-JNK1 and p-paxillin in tumor tissues were correlated with each other. PMID: 24395444
    27. LPS-induced paxillin phosphorylation at Y31 and Y118 was mediated by c-Abl tyrosine kinase PMID: 25795725
    28. This chapter outlines recent advances in understanding how paxillin regulates both steroid and growth factor signaling, focusing on the conserved nature of its actions from a frog germ cell to a human cancer cell. PMID: 25182764
    29. Study provides evidence that phosphorylation of PXN is required for cisplatin resistance in lung cancer cells. PMID: 24096476
    30. High PXN expression is associated with oral cavity squamous cell carcinoma. PMID: 24894864
    31. paxillin may promote cell proliferation and inhibit apoptosis in SW480 cells. Paxillin may be a potential metastasis predictor, and an independent prognosis factor of recurrence. PMID: 24451945
    32. MEKK2 induces paxillin ubiquitylation in breast cancer cells and this function requires both paxillin LD1 motif and MEKK2 kinase activity. PMID: 25190348
    33. Paxillin knockdown increases capillary endothelial cell migration and invasiveness, and thereby enhances microvessel ingrowth, by suppressing NRP2 expression. PMID: 24522185
    34. Through HDAC6-dependent regulation of the microtubule cytoskeleton, paxillin regulates both Golgi organelle integrity and polarized cell invasion. PMID: 25070956
    35. PXN plays an important role in tumor progression and may be used as a potential prognostic indicator in gastric cancer PMID: 24180516
    36. Future studies investigating these hypotheses on TG-2-paxillin relationships are necessary in order to address this fundamental process in cell matrix adhesion signaling. PMID: 24193434
    37. The data suggests that mutant PXN variants play a prominent role in mitochondrial dynamics with direct implications on lung cancer progression. PMID: 23792636
    38. In aneuploid tumors, EZH2 expression and paxillin expression correlate with more aggressive phenotype of breast cancer. PMID: 24344012
    39. ezrin and paxillin may have roles in aggressive tumor features and invasiveness in urothelial bladder tumors PMID: 21868260
    40. The paxillin plays vital roles in cell motility through regulation of focal adhesion dynamics. PMID: 22481092
    41. Lysophosphatidic acid (LPA) induces both time and dose-dependent tyrosine phosphorylation of paxillin and focal adhesion kinase. PMID: 24061591
    42. Paxillin signaling contributed to tumor growth and vasculogenic mimicry of gallbladder carcinomas. PMID: 23588386
    43. Mutation of paxillin serine 250 prevents its phosphorylation by SLK in vitro and results in impaired migration in vivo as evidenced by an accumulation of phospho-FAK-Tyr397 and altered FA turnover rates. PMID: 23128389
    44. Data indicate that lasp-2 interacts with the focal adhesion proteins vinculin and paxillin. PMID: 23389630
    45. overexpression of PXN induced by suppression of miR-137 promotes tumor progression and metastasis and could serve as an independent prognostic indicator in colorectal cancer patients. PMID: 23275153
    46. Paxillin is critical for integrating physical cues from the ECM with chemical motility signals by spatially constraining where cells form motile processes, and thereby regulates directional migration. PMID: 23076140
    47. Fascin-1, ezrin and paxillin contribute to the malignant progression and are predictors of clinical prognosis in laryngeal squamous cell carcinoma. PMID: 23209815
    48. Paxillin is a new regulator protein of pulmonary arterial smooth muscle cell growth. PMID: 22959909
    49. phosphorylation of GIT1 on serine 46 by PKD3 represents a molecular switch by which GIT1 localization, paxillin trafficking, and cellular protrusive activity are regulated. PMID: 22893698
    50. Findings suggest that the MLK3-JNK-paxillin signaling axis may represent a potential prognostic marker in breast cancer metastasis. PMID: 22700880

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  • 亞細(xì)胞定位：
    Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Cytoplasm, cell cortex.
  • 蛋白家族：
    Paxillin family
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 9718

    OMIM: 602505

    KEGG: hsa:5829

    STRING: 9606.ENSP00000228307

    UniGene: Hs.446336