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  4. TRAF2 Recombinant Monoclonal Antibody

TRAF2 Recombinant Monoclonal Antibody

  • 中文名稱：
    TRAF2重組抗體
  • 貨號(hào)：
    CSB-RA786226A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate, 293T whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, K562 whole cell lysate
      All lanes: TRAF2 antibody at 1:1500
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 56, 62, 55, 54 kDa
      Observed band size: 56 kDa
    • IHC image of CSB-RA786226A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA786226A0HU diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
    • Overlay histogram showing Hela cells stained with CSB-RA786226A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1μg/1*106 cells) for 1 h at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1μg/1*106 cells) used under the same conditions. Acquisition of >10,000 events was performed.
    • Immunoprecipitating TRAF2 in Hela whole cell lysate
      Lane 1: Rabbit control IgG instead of CSB-RA786226A0HU in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
      Lane 2: CSB-RA786226A0HU(2μg)+ Hela whole cell lysate(500μg)
      Lane 3: Hela whole cell lysate (10μg)
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    TRAF2 is an important adaptor protein that transduces signals after ligating to certain receptors including those binding TNF and is also an E3 ubiquitin ligase. TRAF2 is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-κB. Studies of TRAF2-deficient mice showed that TRAF2 plays an essential role in mediating cell survival, normal adaptive immune responses, and lymphocyte homeostasis. TRAF2 promotes survival, proliferation, and metastasis of tumor cells through the NF-κB pathway by directly interacting with various TNF receptors.

    Compared with the polyclonal and monoclonal antibodies of TRAF2, this TRAF2 recombinant antibody has the features of increased reproducibility and control, animal-free technology, high degree of monovalency, high batch-to-batch consistency, easier isotype conversion, etc. And it has been validated in ELISA, WB, IHC, FC, IP.

  • Uniprot No.：
  • 基因名：
  • 別名：
    TNF receptor-associated factor 2 (EC 2.3.2.27) (E3 ubiquitin-protein ligase TRAF2) (RING-type E3 ubiquitin transferase TRAF2) (Tumor necrosis factor type 2 receptor-associated protein 3), TRAF2, TRAP3
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from human TRAF2
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號(hào)：
    9A5
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IHC, FC, IP
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
    FC 1:20-1:200
    IP 1:200-1:1000
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Regulates activation of NF-kappa-B and JNK and plays a central role in the regulation of cell survival and apoptosis. Required for normal antibody isotype switching from IgM to IgG. Has E3 ubiquitin-protein ligase activity and promotes 'Lys-63'-linked ubiquitination of target proteins, such as BIRC3, RIPK1 and TICAM1. Is an essential constituent of several E3 ubiquitin-protein ligase complexes, where it promotes the ubiquitination of target proteins by bringing them into contact with other E3 ubiquitin ligases. Regulates BIRC2 and BIRC3 protein levels by inhibiting their autoubiquitination and subsequent degradation; this does not depend on the TRAF2 RING-type zinc finger domain. Plays a role in mediating activation of NF-kappa-B by EIF2AK2/PKR. In complex with BIRC2 or BIRC3, promotes ubiquitination of IKBKE.
  • 基因功能參考文獻(xiàn)：
    1. The TRAIP coiled-coil domain altered its stoichiometry between dimer and trimer in a concentration-dependent manner. Additionally, the TRAIP RING domain induced even higher-ordered assembly, which was necessary for interacting with the TRAF-N domain of TRAF2 but not TRAF1. PMID: 30127245
    2. high levels of CASC9.5 expression promote the proliferation, metastasis and metabolism of lung adenocarcinoma cells and might serve as a prognostic indicator. PMID: 29311633
    3. down-regulation of USP48 increases E-cadherin expression and epithelial barrier integrity through reducing TRAF2 stability PMID: 28874458
    4. DR5-Cbl-b/c-Cbl-TRAF2 complex inhibited TRAIL-induced apoptosis by promoting TRAF2-mediated polyubiquitination of caspase-8 in gastric cancer cells. PMID: 28972304
    5. CPNE1 overexpression can upregulate TRAF2 expression in prostate cancer DU-145 cells as determined by Western blotting and immunofluorescence assays. PMID: 29151113
    6. TRAF2 functions as an important modulator in tumor metastasis and tumor microenvironment formation and is a novel independent prognostic factor of gastric cancer. PMID: 28482378
    7. High expression of TRAF2 can predict poorer prognosis of GBM and was identified as an independent biomarker in GBM prognosis. PMID: 28926524
    8. TRAF2 can influence in vitro growth of TRAIL-treated DU-145 cells at least partially via regulating SIRT1 expression. PMID: 28667915
    9. Intestinal I/R induced the membrane translocation and phosphorylation of PKCzeta. Pretreatment with the PKCzeta activator phosphatidylcholine remarkably attenuated gut injury by suppressing apoptosis. H/R induced PKCzeta to combine with TRAF2, which was phosphorylated by PKCzeta at Ser(55), but not at Ser(11), under intestinal I/R or H/R conditions PMID: 28726782
    10. Both S1P and caspase-8 are critical for TRAF2 stabilization, polyubiquitination, subsequent activation of JNK/AP1 signaling and MMP1 expression and final promotion of cell invasion. PMID: 28482915
    11. Results showed that high expression of TRAF2 was significantly associated with tumor stage of prostate cancer. PMID: 28855498
    12. The addition of nanomolar concentration of TRAF2 in GUVs also seems to exert a mechanical action. PMID: 28499815
    13. TRAF2 and OTUD7B govern a ubiquitin-dependent switch that regulates mTORC2 signalling PMID: 28489822
    14. destabilization of TRAF2 by miR-17 reduced the ability of TRAF2 to associate with cIAP2, resulting in the downregulation of TNF-alpha-induced NF-kappaBp65, c-Jun, and STAT3 nuclear translocation and the production of IL-6, IL-8, MMP-1, and MMP-13 in human rheumatoid arthritis synovial fibroblasts. PMID: 27534557
    15. HOXA1-mediated activation of NF-kappaB is non-transcriptional and the RBCK1 and TRAF2 influences on NF-kappaB are epistatic to HOXA1 PMID: 27382069
    16. RIPK1 collaborates with TRAF2 to inhibit murine and human hepatocarcinogenesis. PMID: 28017612
    17. Study presents the characterization of the peptide binding preferences of TRAFs 2, 3, and 5 using deep mutational scanning. The three TRAF proteins demonstrated different preferences for binding to members of the CD40 library, and three peptides from that library individually showed striking differences in affinity for the three TRAFs. PMID: 26779844
    18. TRAF2 expression was increased in gastric cancer patients as a result of DNA hypomethylation. PMID: 26823737
    19. Data show that when death receptor 5 (DR5) is suppressed, caspase-8 may recruit and stabilize TNF receptor-associated factor 2 (TRAF2) to form a metastasis and invasion signaling complex, resulting in activation of ERK signaling. PMID: 26510914
    20. these findings reveal a novel mechanism by which TRAF2 mediates Lys63-linked ubiquitination of DUSP14, leading to DUSP14 activation in T cells PMID: 26521044
    21. There was a significant positive correlation between TRAF2 and TRAF4 expression levels in malignant pleural effusion cells PMID: 26331901
    22. Data show that ectopic expression of interferon regulatory factor 1 (IRF-1) reduces NF-kappa B activity and suppresses TNF receptor-associated factor 2 (TRAF2) and inhibitor of apoptosis 1 protein (cIAP1) expression in breast cancer cells. PMID: 26011589
    23. The results identify TRAF2 as an important biological suppressor of necroptosis in vitro and in vivo. PMID: 25882049
    24. MAVS50, exposing a degenerate TRAF-binding motif within its N-terminus, effectively competed with full-length MAVS for recruiting TRAF2 and TRAF6 PMID: 26221961
    25. Presented is the trimer-to-monomer equilibrium transition of the TRAF2 C-terminal domain using both chemical (dilution/guanidinium hydrochloride) and mechanical stress (high pressure) to induce the dissociation of the native protein into subunits. PMID: 26390021
    26. Knockdown of IRE1alpha by siRNA dramatically abrogated CXC195-induced activation of TRAF2, ASK and JNK, formation of an IRE1alpha-TRAF2-ASK1 complex and caspase- and mitochondrial-dependent apoptosis in T24 cells PMID: 25797626
    27. PP4R1 could mediate the dephosphorylation of TRAF2 Ser11. PMID: 25134449
    28. TRAF2 acts as an antagonist of TRAIL- and CD95L-induced necroptosis. PMID: 25299769
    29. Data indicate that TRAF interacting protein TRIP negatively regulates the TNFR-associated factor 2 (TRAF2) ubiquitin-dependent pathway by modulating the TRAF2-sphingosine 1-phosphate (S1P) interaction. PMID: 25716317
    30. The phosphorylation of TRAF2 at serine 11 is essential for the survival of cancer cells harboring TRAF2 amplification. Observations identify TRAF2 as a frequently amplified oncogene. PMID: 24362534
    31. preventing CD40-TRAF2,3 or CD40-TRAF6 interaction inhibits pro-inflammatory responses in human non-haematopoietic cells. PMID: 25051892
    32. During stress-induced mitochondrial hyperfusion, MULAN forms a complex with TRAF2 and modulates its ubiquitylation, signifying that TRAF2 may serve as an ubiquitylated transmitter of NF-kappaB signaling in this pathway PMID: 24841215
    33. GSTP1-1's TRAF2-binding region involves the GSH-binding site. PMID: 24457959
    34. RNASET2 contributes to vitiligo pathogenesis by inhibiting TRAF2 expression. PMID: 24457966
    35. Data indicate that in monocyte- derived macrophages (MDMs) acutely infected with HIV-1 and treated with HCV rCore and HIV-1 rNef, the HIV-1 replication depends on an upstream signal mediated through TRAF2, TRAF5 and TRAF6. PMID: 23774506
    36. TRAF2 is a proviral factor for vaccinia virus that plays a role in promoting efficient viral entry, most likely via the plasma membrane. PMID: 24429366
    37. IpaH0722 dampens the acute inflammatory response by preferentially inhibiting the PKC-mediated activation of NF-kappaB by ubiquitinating TRAF2, a molecule downstream of PKC, and by promoting its proteasome-dependent degradation. PMID: 23754945
    38. it is hypothesized that ZC3H15 may interact with TRAF-2 functionally within the NF-kappaB pathway, and may be explored as a potential target in acute myeloid leukemia PMID: 23624947
    39. The results of this study showed TRAF2 is upregulated in relapsing-remitting multiple sclerosis. PMID: 23595117
    40. TRAF2 can predict relapse of ulcerative colitis PMID: 23638224
    41. Data indicate that the Src homology 3 domain-containing GEF (SGEF) promotes fibroblast growth factor-inducible 14 (Fn14) proinvasive signaling in glioblastoma via TNF receptor-associated factor 2 (TRAF2). PMID: 23775076
    42. Authors found that rotavirus Non-Structural Protein-1 (NSP1) interacts with TRAF2 and degrades it in a proteasome dependent manner. PMID: 23890813
    43. activation of TRAF-1 may be early events in the pathogenesis of inflammatory bowel disease PMID: 23414308
    44. Data iindicate that TRAF2 affects the localization and function of TRAF4 in breast cancer cell lines. PMID: 23743189
    45. TRAF2/NIK/NF-kappaB2 pathway regulates pancreatic ductal adenocarcinoma cell tumorigenicity PMID: 23301098
    46. Parkin suppresses inflammation and cytokine-induced cell death by promoting the proteasomal degradation of TRAF2/6. PMID: 23608757
    47. RAD23A down-regulated TRAF2 protein level through ubiquitin-proteasome system. PMID: 23357418
    48. These results reveal a critical role for the MAPK8 activation pathway through TRAF2 and RIPK1 for TNFSF10-induced autophagy that blunts apoptosis in cancer cells. PMID: 23051914
    49. Changes in expression levels of TNFSF4 and TRAF2 mRNAs may significantly correlate with the pathogenesis of SLE, the disease activity and different clinical features of lupus, particularly lupus nephritis, atherosclerosis and arthritis PMID: 22847298
    50. A novel radioresistance mechanism through functional orchestration of rpS3, TRAF2, and NF-kappaB in non-small cell lung cancer cells, is reported. PMID: 23188828

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  • 亞細(xì)胞定位：
    Cytoplasm.
  • 蛋白家族：
    TNF receptor-associated factor family, A subfamily
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 12032

    OMIM: 601895

    KEGG: hsa:7186

    STRING: 9606.ENSP00000247668

    UniGene: Hs.522506