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  4. Recombinant Arabidopsis thaliana Cleavage and polyadenylation specificity factor CPSF30 (CPSF30), partial

Recombinant Arabidopsis thaliana Cleavage and polyadenylation specificity factor CPSF30 (CPSF30), partial

  • 中文名稱：
    Recombinant Arabidopsis thaliana Cleavage and polyadenylation specificity factor CPSF30(CPSF30) ,partial,Yeast
  • 貨號：
    CSB-YP429350DOA
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana Cleavage and polyadenylation specificity factor CPSF30(CPSF30) ,partial,Yeast
  • 貨號：
    CSB-EP429350DOA
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana Cleavage and polyadenylation specificity factor CPSF30(CPSF30) ,partial,Yeast
  • 貨號：
    CSB-EP429350DOA-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana Cleavage and polyadenylation specificity factor CPSF30(CPSF30) ,partial,Yeast
  • 貨號：
    CSB-BP429350DOA
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana Cleavage and polyadenylation specificity factor CPSF30(CPSF30) ,partial,Yeast
  • 貨號：
    CSB-MP429350DOA
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    CPSF30
  • Uniprot No.：
  • 別名：
    CPSF30; OXT6; At1g30460; F26G16.5; F26G16.630-kDa cleavage and polyadenylation specificity factor 30; EC 3.1.21.-; Protein OXIDATIVE STRESS TOLERANT 6; Zinc finger CCCH domain-containing protein 11; AtC3H11
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation. May interact with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Mediates poly(A) site selection. Binds RNA in a calcium-dependent manner. Exhibits endonuclease activity with an ability to nick and degrade linear as well as circular single-stranded RNA that leaves RNA 3' ends with hydroxyl groups, thus mediating processing of the pre-mRNA as a prelude to the polyadenylation. Involved in the post-transcriptional control, probably via poly(A) addition, of the responses of plants to stress, especially genes mediating tolerance to oxidative stress. Plays a role in the regulation of salicylic acid (SA) production via the control of messenger RNA 3' end processing, thus being a key component of programmed cell death and plant immune responses required for resistance to virulent Pseudomonas syringae pv tomato DC3000 (Pst).
  • 基因功能參考文獻：
    1. The 65 kDa protein encoded by CPSF30-L mediates nitrate signaling in part by regulating NRT1.1 expression. PMID: 28850721
    2. CPSF30 mutant incapable of interacting with calmodulin has reduced fertility, reduced lateral root formation, and altered sensitivities to oxidative stress. PMID: 25546057
    3. review of role in mRNA polyadenylation and cellular signaling PMID: 25104048
    4. CPSF30 mediates alternative polyadenylation in Arabidopsis. PMID: 23136375
    5. the presence of a disulfide linkage in the endonuclease motif of AtCPSF30 protein PMID: 20888817
    6. Results indicate that AtCPSF30 is a probable processing endonuclease, and that its action is coordinated through its interaction with Fip1. PMID: 17576667
    7. results suggest that the nuclear CPSF complex in plants is a dynamic one, and that the interactions between AtCPSF30 and other CPSF subunits are different from those existing in other eukaryotes. PMID: 19573236

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  • 亞細胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    CPSF4/YTH1 family
  • 組織特異性：
    Expressed in seedlings, roots, leaves, siliques, stems and flowers.
  • 數(shù)據(jù)庫鏈接：

    KEGG: ath:AT1G30460

    STRING: 3702.AT1G30460.1

    UniGene: At.40546