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  4. Recombinant Arabidopsis thaliana DNA-directed RNA polymerase II subunit RPB7 (RPB19)

Recombinant Arabidopsis thaliana DNA-directed RNA polymerase II subunit RPB7 (RPB19)

  • 中文名稱：
    擬南芥NRPB7重組蛋白
  • 貨號：
    CSB-YP018334DOA
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥NRPB7重組蛋白
  • 貨號：
    CSB-EP018334DOA
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥NRPB7重組蛋白
  • 貨號：
    CSB-EP018334DOA-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥NRPB7重組蛋白
  • 貨號：
    CSB-BP018334DOA
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥NRPB7重組蛋白
  • 貨號：
    CSB-MP018334DOA
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    NRPB7
  • Uniprot No.：
  • 別名：
    NRPB7; RPB19; At5g59180; MNC17.9; DNA-directed RNA polymerase II subunit 7; RNA polymerase II subunit B7
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    full length protein
  • 表達區(qū)域：
    1-176
  • 氨基酸序列
    MFFHIVLERN MQLHPRFFGR NLKENLVSKL MKDVEGTCSG RHGFVVAITG IDTIGKGLIR DGTGFVTFPV KYQCVVFRPF KGEILEAVVT LVNKMGFFAE AGPVQIFVSK HLIPDDMEFQ AGDMPNYTTS DGSVKIQKEC EVRLKIIGTR VDATAIFCVG TIKDDFLGVI NDPAAA
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. NRPB7 is part of a subcomplex with NRPB4 that binds to a pocket formed by NRPB1, NRPB2 and NRPB6 at the base of the clamp element. The NRBP4-NRPB7 subcomplex seems to lock the clamp via NRPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The NRPB4-NRPB7 subcomplex binds single-stranded DNA and RNA.
  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    Eukaryotic RPB7/RPC8 RNA polymerase subunit family
  • 數據庫鏈接：

    KEGG: ath:AT5G59180

    STRING: 3702.AT5G59180.1

    UniGene: At.29242