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  4. Recombinant Arabidopsis thaliana Phytochrome B (PHYB), partial

Recombinant Arabidopsis thaliana Phytochrome B (PHYB), partial

  • 中文名稱：
    擬南芥PHYB重組蛋白
  • 貨號：
    CSB-YP318575DOA
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥PHYB重組蛋白
  • 貨號：
    CSB-EP318575DOA
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥PHYB重組蛋白
  • 貨號：
    CSB-EP318575DOA-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥PHYB重組蛋白
  • 貨號：
    CSB-BP318575DOA
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥PHYB重組蛋白
  • 貨號：
    CSB-MP318575DOA
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    PHYB
  • Uniprot No.：
  • 別名：
    PHYB; HY3; OOP1; At2g18790; MSF3.17; Phytochrome B; Protein LONG HYPOCOTYL 3; Protein OUT OF PHASE 1
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Regulatory photoreceptor which exists in two forms that are reversibly interconvertible by light: the Pr form that absorbs maximally in the red region of the spectrum and the Pfr form that absorbs maximally in the far-red region. Photoconversion of Pr to Pfr induces an array of morphogenetic responses, whereas reconversion of Pfr to Pr cancels the induction of those responses. Pfr controls the expression of a number of nuclear genes including those encoding the small subunit of ribulose-bisphosphate carboxylase, chlorophyll A/B binding protein, protochlorophyllide reductase, rRNA, etc. It also controls the expression of its own gene(s) in a negative feedback fashion. Involved in the flowering time regulation. Involved in light-regulated circadian phase control that triggers stomatal aperture, stomatal conductance, and CO(2) assimilation. Implicated in red light perception, and, to a lower extent, in blue light signaling. Regulates temperature responses by associating with the promoters of key target genes in a temperature-dependent manner and subsequently repressing their expression probably in a PIF4-dependent manner. Thermal timer that integrates temperature information over the course of the night.
  • 基因功能參考文獻：
    1. A family of nuclear protein kinases, designated Photoregulatory Protein Kinases (PPK1-4; formerly called MUT9-Like Kinases (MLKs)), that interact with PIF3 and phyB in a light-induced manner in vivo have been identified. PMID: 28492231
    2. This study demonstrates that the dimeric form of the C-terminal module plays important signaling roles by targeting PHYB to subnuclear photobodies and interacting with PIF3 to trigger its degradation. PMID: 29199270
    3. UVR8 and its mediated signaling pathway via interaction with COP1 can be induced by He-Ne laser, and these processes were dependent on cytoplasmic PhyB levels in plant cells. PMID: 29138939
    4. RVE1 and RVE2 transcription factors promote primary seed dormancy and repress red/far-red-light-reversible germination downstream of phytochrome B (phyB) in Arabidopsis thaliana. RVE1 and RVE2 expression is downregulated after imbibition and by phyB. PMID: 27506149
    5. Mutant analyses and genetic complementation assays show that Phytochrome B is a negative regulator of nucleus size. PMID: 28592555
    6. Mapping light-driven conformational changes within the photosensory module of plant phytochrome B. PMID: 27694986
    7. PhyB enhanced the GEF activity of RopGEF2 toward both ROP7 and ROP2 under light. PMID: 28188273
    8. a null mutation of the JAZ10 gene in Arabidopsis did not affect plant growth nor did it suppress the shade-avoidance responses elicited by phyB inactivation. PMID: 27943325
    9. Light was efficiently conducted through the stems to the roots, where photoactivated phytochrome B (phyB) triggered expression of ELONGATED HYPOCOTYL 5 (HY5) and accumulation of HY5 protein, a transcription factor that promotes root growth in response to light. PMID: 27803284
    10. Genetic evidence indicated that HDA15 acts downstream of PHYB and represses seed germination in the dark dependent on PIF1. PMID: 28444370
    11. phytochrome B (phyB) directly associates with the promoters of key target genes in a temperature-dependent manner. The rate of phyB inactivation is proportional to temperature in the dark, enabling phytochromes to function as thermal timers that integrate temperature information over the course of the night. PMID: 27789797
    12. this study proposes that in addition to its photoreceptor functions, phyB is a temperature sensor in plants. PMID: 27789798
    13. The light-activated association of phyB, EIN3, and EBF1/EBF2 proteins stimulates robust EIN3 degradation by SCF(EBF1/EBF2) E3 ligases. We reveal that phyB manipulates substrate-E3 ligase interactions in a light-dependent manner, thus directly controlling the stability of EIN3. PMID: 27889482
    14. Surprisingly, none of the phytochromes alone conferred a photoperiodic response. Although phyE and phyB were the strongest repressors of flowering, both phyB and phyC were needed to confer a flowering response to photoperiod. Thus, a specific combination of phytochromes is required to detect changes in photoperiod PMID: 27820825
    15. UV-C radiation treatment caused a disturbance in photosystem II and a deregulation of photosynthetic pigment content and antioxidant enzymes activities, followed by increased cell mortality rate in phyB and phyAB null mutants. PMID: 26385378
    16. PhyB acts in the phloem to regulate flowering time.PHYB and HOS1 regulate flowering time additively. PMID: 26373454
    17. the YHB mutation is sufficient to phenocopy red light input into the circadian mechanism and to sustain robust rhythms in steady-state mRNA levels even in plants grown without light or exogenous sugars. PMID: 26157113
    18. High-level expression and phosphorylation of phytochrome B modulates flowering time in Arabidopsis. PMID: 26120968
    19. Data indicate that both phyA and phyB promote seedling photomorphogenesis in a synergistic manner in high-irradiance white light and act antagonistically under far-red light. PMID: 26030677
    20. Interaction of phyB with SPA1 promotes COP1-SPA1 dissociation and photomorphogenic development. PMID: 25744387
    21. Phytochrome B senses the temperature or mediates signal transduction of temperature effects. PMID: 25289638
    22. phytochromeB expression in the stomatal lineage, mesophyll, and phloem is sufficient to restore wild-type stomatal development. Induction of PHYB in mature leaves also rescues stomatal development in young untreated leaves. PMID: 24835461
    23. phyB inhibits leaf flatness; phyB is required for the curled leaf phenotype of the phot1phot2 mutant PMID: 25009300
    24. Low low red : far-red (R : FR) ratios and the phyB mutation stabilized the protein JAZ10. PMID: 25103816
    25. photo-excited phyB is required for stabilization of the COL7 protein PMID: 24908267
    26. A mutation within the phyB gene confers resistance to the herbicide glyphosate. PMID: 24654847
    27. PIF3 lies downstream of PHYB and RGL3, and plays an important role in the inhibitory effect of NO on root growth of Arabidopsis seedlings in light. PMID: 24157606
    28. phyB suppresses auxin signaling to promote branching. PMID: 24492336
    29. ATHB23 transcription factor is a novel component of the phyB-mediated red light signaling pathway. PMID: 23964902
    30. this Arabidopsis PhyB structure should enable molecular insights into plant Phy signaling and provide an essential scaffold to redesign their activities for agricultural benefit and as optogenetic reagents PMID: 24982198
    31. ABP1 and phyB participate auxin and red light signalling. PMID: 24052532
    32. PHYTOCHROME B restricts ethylene biosynthesis and constrains the -day/night temperature difference-induced phase shift in rhythmic growth. PMID: 23979970
    33. Brief heat shocks enhance the inhibition of hypocotyl growth induced by light perceived by phytochrome B in deetiolating Arabidopsis thaliana seedlings. PMID: 23933882
    34. From a phyB null mutant background, singly and doubly transgenic plants are generated that express fusion proteins containing the phyB-phyE N-terminal photosensory regions. PMID: 23738620
    35. Data show that photoreceptor phyB-induced transcription factor PIF3 phosphorylation is required for the known negative feedback modulation of phyB levels in prolonged light, potentially through codegradation of phyB and PIF3. PMID: 23903316
    36. Phosphorylation of phytochrome B inhibits light-induced signaling via accelerated dark reversion in Arabidopsis. PMID: 23378619
    37. PHYB plays a role in repressing far-red (FR) light signaling. Activity modulation of the COP1-SPA E3 complex by light-activated phytochromes is an effective and pivotal regulatory step in light signaling. PMID: 23371951
    38. A collection of phyB mutants was generated affecting the bilin-binding pocket that altered photochemistry, thermal stability, and/or nuclear localization patterns, some of which also impacted phenotypic outputs. PMID: 23321421
    39. Phyb promoted the development of curled leaves. PMID: 23054390
    40. Data indicate that the green light (GL) opposition of red light (RL) responses persists in phyA, phyB, cry1cry2 and phot2 mutants, and the response requires phot1 and NPH3. PMID: 23007554
    41. PhyA and phyB are involved in the regulation of hypocotyl elongation under red and far-red illumination. PMID: 23456246
    42. PhyB inhibits the regulatory activity of PIF1 and PIF3 by releasing them from their DNA targets. PMID: 22849408
    43. PhyB functions at all stages of development including seed germination and seedling establishment, mediates classical red/far-red reversible low fluence responses (LFR) as well as red light high irradiance responses. PMID: 22836504
    44. Deletion of the RS domain of RRC1 reduces phyB signaling. PMID: 22751357
    45. PhyB contributes to the acclimation of plants to open environments by enhancing abscisic acid sensitivity when soil water becomes limiting. PMID: 22553988
    46. LRB1 (LIGHT-RESPONSE BTB1) and LRB2 (LIGHT-RESPONSE BTB2) are involved in many developmental processes mediated by phyB and phyD. PMID: 22732244
    47. in plants, the phyB/CRY1 interaction may mediate cross-talk between the red/far-red- and blue/UV-sensing pathways, enabling fine-tuning of light responses to different spectral inputs PMID: 22577138
    48. Deletion of the RS domain of RRC1 (reduced red-light responses in cry1cry2 background 1), reduces phytochrome B-mediated light responses in a phytochrome B-dependent manner. PMID: 22324426
    49. In the dark, cellulose synthase (CESA) complex (CSC) velocity is inhibited through an interaction with microtubules; activation of PHYTOCHROME B overrules it. Analysis of cesa5 mutants shows a role for phosphorylation in the control of CSC velocity. PMID: 22036185
    50. The nuclear import of phyB can be facilitated by phytochrome-interacting factor 3 (PIF3). PMID: 22451940

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  • 亞細胞定位：
    Cytoplasm. Nucleus, nucleoplasm. Nucleus speckle. Nucleus.
  • 蛋白家族：
    Phytochrome family
  • 數(shù)據(jù)庫鏈接：

    KEGG: ath:AT2G18790

    STRING: 3702.AT2G18790.1

    UniGene: At.96