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  4. Recombinant Autographa californica nuclear polyhedrosis virus Trans-activating transcriptional regulatory protein (IE1), partial

Recombinant Autographa californica nuclear polyhedrosis virus Trans-activating transcriptional regulatory protein (IE1), partial

  • 中文名稱：
    Recombinant Autographa californica nuclear polyhedrosis virus Trans-activating transcriptional regulatory protein(IE1) ,partial,Yeast
  • 貨號：
    CSB-YP318109ARA
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Autographa californica nuclear polyhedrosis virus Trans-activating transcriptional regulatory protein(IE1) ,partial,Yeast
  • 貨號：
    CSB-EP318109ARA
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Autographa californica nuclear polyhedrosis virus Trans-activating transcriptional regulatory protein(IE1) ,partial,Yeast
  • 貨號：
    CSB-EP318109ARA-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Autographa californica nuclear polyhedrosis virus Trans-activating transcriptional regulatory protein(IE1) ,partial,Yeast
  • 貨號：
    CSB-BP318109ARA
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Autographa californica nuclear polyhedrosis virus Trans-activating transcriptional regulatory protein(IE1) ,partial,Yeast
  • 貨號：
    CSB-MP318109ARA
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    IE1
  • Uniprot No.：
  • 別名：
    IE1; Trans-activating transcriptional regulatory protein; Immediate early protein 1; IE-1
  • 種屬：
    Autographa californica nuclear polyhedrosis virus (AcMNPV)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Regulatory transcriptional protein, which trans-activates gene expression from early baculovirus promoters. Can also trans-activate its own promoter, suggesting an autoregulation during infection of host cells. Promotes also viral DNA genome replication via the N-terminal region.
  • 基因功能參考文獻：
    1. Phosphorylation sites in the IE1 replication domain are required for virus multiplication. PMID: 22496221
    2. These results suggest that IE1 regulates gp64 early promoters to produce the proper amount of GP64 protein, depending upon nucleopolyhedrovirus-insect cell systems. PMID: 20837073
    3. Ie1-deficient mutant abrogates viral gene expression and can not replicate in insect host cells. PMID: 20705310
    4. We conclude that a unique cooperative mechanism of ie1 and p35 exists in the genome of AcMNPV, which can activate the expression of a specific set of AcMNPV and HzNV-1 promoters PMID: 18486255
    5. This study reveals that IE1 is a gene-selective transcriptional activator which is required not only for expedition of virus multiplication but also for blocking of its own proapoptotic activity by upregulation of baculovirus apoptotic suppressors. PMID: 18945761

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  • 亞細胞定位：
    Host nucleus.
  • 蛋白家族：
    Nucleopolyhedrovirus IE-1 protein family
  • 數(shù)據(jù)庫鏈接：

    KEGG: vg:1403980