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  4. Recombinant Bacillus subtilis Chaperone protein DnaJ (dnaJ)

Recombinant Bacillus subtilis Chaperone protein DnaJ (dnaJ)

  • 中文名稱：
    枯草芽孢桿菌dnaJ重組蛋白
  • 貨號：
    CSB-YP321543BRJ
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    枯草芽孢桿菌dnaJ重組蛋白
  • 貨號：
    CSB-EP321543BRJ
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    枯草芽孢桿菌dnaJ重組蛋白
  • 貨號：
    CSB-EP321543BRJ-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    枯草芽孢桿菌dnaJ重組蛋白
  • 貨號：
    CSB-BP321543BRJ
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    枯草芽孢桿菌dnaJ重組蛋白
  • 貨號：
    CSB-MP321543BRJ
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    dnaJ
  • Uniprot No.：
  • 別名：
    dnaJ; BSU25460Chaperone protein DnaJ
  • 種屬：
    Bacillus subtilis (strain 168)
  • 蛋白長度：
    full length protein
  • 表達區(qū)域：
    1-375
  • 氨基酸序列
    MSKRDYYEVL GVSKSASKDE IKKAYRKLSK KYHPDINKEA GSDEKFKEVK EAYETLSDDQ KRAHYDQFGH TDPNQGFGGG GFGGGDFGGF GFDDIFSSIF GGGTRRRDPN APRQGADLQY TMTLSFEDAA FGKETTIEIP REETCETCKG SGAKPGTNPE TCSHCGGSGQ LNVEQNTPFG KVVNRRVCHH CEGTGKIIKN KCADCGGKGK IKKRKKINVT IPAGVDDGQQ LRLSGQGEPG INGGPAGDLF VVFHVRAHEF FERDGDDIYC EMPLTFAQAA LGDEVEVPTL HGKVKLKIPA GTQTGTKFRL RGKGVQNVRG YGQGDQHIVV RVVTPTNLTD KQKDIIREFA EVSGNLPDEQ EMSFFDKVKR AFKGD
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.
  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    DnaJ family
  • 數據庫鏈接：

    KEGG: bsu:BSU25460