E. coli biotin ligase
(BirA) is highly specific in covalently attaching biotin to the 15 amino
acid AviTag peptide. This recombinant protein was biotinylated in vivo
by AviTag-BirA technology, which method is BriA catalyzes amide linkage
between the biotin and the specific lysine of the AviTag.
The tag type will be
determined during production process. If you have specified tag
type, please tell us and we will develop the specified tag
preferentially.
產(chǎn)品提供形式:
Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶:
We recommend that this vial be briefly centrifuged prior
to opening to bring the contents to the bottom. Please reconstitute
protein in deionized sterile water to a concentration of 0.1-1.0
mg/mL.We recommend to add 5-50% of glycerol (final concentration) and
aliquot for long-term storage at -20℃/-80℃. Our default final
concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for
mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期:
The shelf life is related to many factors, storage state,
buffer ingredients, storage temperature and the stability of the protein
itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The
shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期:
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our
proteins are default shipped with normal blue ice packs, if you
request to ship with dry ice, please communicate with us in advance
and extra fees will be charged.
注意事項(xiàng):
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Differential cell-specific and spatiotemporal expression of the EDN1 system and NOS in the bovine utero-placental unit may be associated with regulation of vascular and cellular functions during pregnancy. PMID: 22921266
Regulated during different stages of cycle. Hormonal regulation and intracellular localization of bovine ECE-1 isoforms may provide new insights into endothelin 1 biosynthesis and mode of action in different cellular microenvironments within ovary. PMID: 12606425
It is suggested that endothelial cells sense shear stress as oxidative stress and transduce signal for the regulation of the gene expression of ECE. PMID: 12609744
presence of Endothelin-converting enzyme-1 isoforms in different endothelial cell compartments than full-length forms of the enzyme may suggest a distinct physiological role for these proteins PMID: 16186113
亞細(xì)胞定位:
Cell membrane; Single-pass type II membrane protein.