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  4. Recombinant Chicken anemia virus Apoptin (VP3)

Recombinant Chicken anemia virus Apoptin (VP3)

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  • 中文名稱：
    Recombinant Chicken anemia virus Apoptin (VP3)
  • 貨號：
    CSB-EP346512CIF
  • 規(guī)格：
    ￥2328
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP346512CIF could indicate that this peptide derived from E.coli-expressed Chicken anemia virus (isolate USA 26p4) (CAV) VP3.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP346512CIF could indicate that this peptide derived from E.coli-expressed Chicken anemia virus (isolate USA 26p4) (CAV) VP3.
  • 其他：

產品詳情

  • 純度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    VP3
  • Uniprot No.：
  • 別名：
    VP3; Apoptin
  • 種屬：
    Chicken anemia virus (isolate USA 26p4) (CAV)
  • 蛋白長度：
    Full Length
  • 來源：
    E.coli
  • 分子量：
    17.1kDa
  • 表達區(qū)域：
    1-120aa
  • 氨基酸序列
    MNALQEDTPPGPSTVFRPPTSSRPLETPHCEIRIGIAGITITLSLCGCANARAPTLRSATADSSESTGFKNAPDLRTDQPKPPSKKRSCDPSEYRVSELKENLITTTPSRPRTARRCIRL
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標簽：
    N-terminal 6xHis-tagged
  • 產品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    3-7 business days
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產品描述：

    The expression vector recombined with the recombinant DNA was transformed into the E.coli for expression. The recombinant DNA resulted from the fusion of the gene coding for the 1-120aa of the chicken anemia virus VP3 protein and the N-terminal 6xHis tag gene. The product was purified and isolated to get the recombinant chicken anemia virus VP3 protein with N-terminal 6xHis tag. The purity of this recombinant VP3 protein reaches up to 90%. Under SDS-PAGE condition, this recombinant VP3 protein showed a band with a molecular weight of about 20 kDa on the gel. It may be used in the microbiology studies.

    VP3 is a gene encoding a protein named chicken anemia virus Apoptin (abbreviated Apoptin) and belongs gyrovirus apoptin family. This protein is 121 amino acids in length and has a mass of 13.6 kDa and contains a bipartite C-terminal nuclear localization signal (NLS) from residues 82–88 and 111–121, which permits its translocation to the nucleus in a cell-type-specific manner. Apoptin infects the thymocytes and erythroblasts in young chickens, causing chicken infectious anemia and immunosuppression. Apoptin has subsequently been shown to induce apoptosis in many human cancer cell lines in a manner that is independent of p53. Apoptin's tumor-cell killing ability has generated significant interest for its potential in anticancer therapy, and indeed apoptin has been investigated extensively in a wide range of human tumor cell.

  • Datasheet & COA：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    May act as transcriptional regulator. Induces apoptosis in infected cells. Element of infectious replication cycle.
  • 亞細胞定位：
    Host nucleus. Note=Host nucleus of infected cells.
  • 蛋白家族：
    Gyrovirus apoptin family