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  4. Recombinant Chromobacterium violaceum tRNA (Ile)-lysidine synthase (tilS)

Recombinant Chromobacterium violaceum tRNA (Ile)-lysidine synthase (tilS)

  • 中文名稱：
    紫羅蘭染色體tilS重組蛋白
  • 貨號：
    CSB-YP752467CKA
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    紫羅蘭染色體tilS重組蛋白
  • 貨號：
    CSB-EP752467CKA
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    紫羅蘭染色體tilS重組蛋白
  • 貨號：
    CSB-EP752467CKA-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    紫羅蘭染色體tilS重組蛋白
  • 貨號：
    CSB-BP752467CKA
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    紫羅蘭染色體tilS重組蛋白
  • 貨號：
    CSB-MP752467CKA
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    tilS
  • Uniprot No.：
  • 別名：
    tilS; CV_3189; tRNA(Ile)-lysidine synthase; EC 6.3.4.19; tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase
  • 種屬：
    Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757)
  • 蛋白長度：
    full length protein
  • 表達區(qū)域：
    1-435
  • 氨基酸序列
    MADSLIEHWP DELSGLFAFE VGFSGGLDSV ALLSLLCEAR ARRPEIGLSA VHVHHGLSPN ADAWAAHCQA LCDSLRVPLR IERVHVRAGG GESVEAAARE ARYQVYRRSA SEVIALAHHQ DDQAETILLQ LLRGGGARAL AAMPALRELA PGKLLWRPLL EIPRERLEAY VKARGFAWVD DESNLDTRYR RNLLRHDILP RLERALPHYR SHLARAAMLQ ADAAAILDEV AREDLQACRT ENGLDCGSVL ALSAPRRRQT LLAWLDALGW PAPEPGALLE FLRQAEYAER GASPVLRLRQ GCLLRFADTL QAWPKAQSEP PASTALLWRG GDAQSLSEWG GELAWERRDA GVPAALLESG AYLAPRRGGE KLSARVGRRE VKDLLREAGV PPLLRRRWPL LYGADGELLA VPGIAVSYRA AADGGWWPLW RPARP
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine.
  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    TRNA(Ile)-lysidine synthase family
  • 數(shù)據(jù)庫鏈接：

    KEGG: cvi:CV_3189

    STRING: 243365.CV_3189