Recombinant DT3C (Diphtheria toxin & spg 3C domain) for Antibody Internalization Assay (Active)
In Stock-
中文名稱:
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貨號:CSB-EP360556CQR1
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規(guī)格:
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圖片:
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
After DT3C 10μg/ml formed complexes with different concentrations of Anti-CCR8 antibodies (CSB-RA004847MA3HU), cytotoxicity experiments were conducted on CHOK1/Human CCR8 cells (CSB-SC004847HU2). The ED50 is 0.3708-0.5608 μg/ml. Biological Activity Assay -
Activity
After DT3C 10μg/ml formed complexes with different concentrations of Anti-CCR8 antibodies (CSB-RA004847MA3HU), cytotoxicity experiments were conducted on CT26/Human CCR8 cells (CSB-SC004847HU3). The ED50 is 0.4661-0.8614 μg/ml. Biological Activity Assay
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產品詳情
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純度:Greater than 90% as determined by SDS-PAGE.Greater than 90% as determined by SEC-HPLC.
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內毒素:Less than 1.0 EU/ug as determined by LAL method.
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生物活性:①After DT3C 10μg/ml formed complexes with different concentrations of Anti-CCR8 antibodies (CSB-RA004847MA3HU), cytotoxicity experiments were conducted on CHOK1/Human CCR8 cells (CSB-SC004847HU2). The ED50 is 0.3708-0.5608 μg/ml.②After DT3C 10μg/ml formed complexes with different concentrations of Anti-CCR8 antibodies (CSB-RA004847MA3HU), cytotoxicity experiments were conducted on CT26/Human?CCR8?cells?(CSB-SC004847HU3). The ED50 is 0.4661-0.8614 μg/ml.
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基因名:DT3C
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Uniprot No.:
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種屬:N/A
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蛋白長度:Linked Heterodimer
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來源:E.coli
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分子量:69.4 kDa
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表達區(qū)域:33-417aa(P00588)+linker(GGGGSGGGGSGGGGS)+291-497aa (P19909)
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氨基酸序列GADDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYDDDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKELGLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEINFETRGKRGQDAMYEYMAQACAGNRVRRSVGSSLSCINLDWDVIRDKTKTKIESLKEHGPIKNKMSESPNKTVSEEKAKQYLEEFHQTALEHPELSELKTVTGTNPVFAGANYAAWAVNVAQVIDSETADNLEKTTAALSILPGIGSVMGIADGAVHHNTEEIVAQSIALSSLMVAQAIPLVGELVDIGFAAYNFVESIINLFQVVHNSYNRPAYSPGHKGGGGSGGGGSGGGGSIDEILAALPKTDTYKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDDATKTFTVTEKPEVIDASELTPAVTTYKLVINGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDDATKTFTVTEKPEVIDASELTPAVTTYKLVINGKTLKGETTTKAVDAETAEKAFKQYANDNGVDGVWTYDDATKTFTVTE
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蛋白標簽:N-terminal?6xHis-tagged
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產品提供形式:Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
緩沖液:Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
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復溶:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
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儲存條件:Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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保質期:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期:Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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注意事項:Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
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Datasheet & COA:Please contact us to get it.
產品評價
實驗應用:抗體內化實驗
產品評價: 我購買了CSB-EP360556CQR1用于抗體內化檢測,使用了CUSABIO推薦的實驗流程,實驗流程很詳細,服務也很周到。我用DT3C在不同的細胞中檢測,并顯示出不同的效果。我發(fā)現不同類型的細胞對DT3C的敏感度不同,其中一些細胞在抗體內化測定中顯示出更好的結果??贵w內化的效率與抗原密度、抗體親和力以及抗體和抗原結合表位密切相關。針對同一靶標的不同抗體也會表現出不同的內化效率。因此,建議在早期階段可以多測試幾個細胞。
By 張老師
實驗應用:抗體內化實驗
產品評價: 用CUSABIO的DT3C產品進行抗體內化效率檢測,與其他方法相比,其mAb-DT3C復合物制備簡單,且其抗體內化效率高于Mab-ZAP,性價比較高。我們會再次購買。
By 何老師
實驗應用:細胞實驗
產品評價: 我購買了DTC3重組蛋白用于雜交瘤細胞篩選,參考了某文獻中的實驗流程,結果很理想。具體實驗流程如下: 用DT3C對雜交瘤細胞進行篩選:取96孔板,向每個孔中加入4μg/mL的DT3C(25微升),然后加入得到的雜交瘤的培養(yǎng)上清液(25微升),混勻后在室溫下孵育30分鐘。將NOR-P1細胞以2 x 10^5細胞/mL的濃度(RPMI培養(yǎng)基,含10%低IgG FBS (+))接種,然后在37℃的CO2培養(yǎng)箱中培養(yǎng)3天。培養(yǎng)后通過顯微鏡觀察,確定粘附細胞數約為使用陰性對照抗體時粘附細胞數的25%或更少的孔為陽性。將選定的克隆進行一個或兩個亞克隆步驟,以建立8個單克隆雜交瘤細胞系。
By 何老師
問答及客戶評論
請給我們關于處理這個產品重組DT3C蛋白(CSB-EP360556CQR1)的建議,推薦的生物安全級別(BSL)是多少?
我對你們的產品DT3C重組蛋白用于抗體內化檢測很感興趣,想知道這對于膠質母細胞瘤是否有效?
如果在孵育DT3C和抗體時,存在無血清培養(yǎng)基,是否會影響它們的結合?
DT3C蛋白能夠檢測ADC藥物抗體的內吞效率嗎?
DT3C的有效濃度是多少?
DT3C和抗體的結合表位是什么?
DT3C蛋白的特異性是什么(添加后對細胞的影響)?
您是否檢查了您的重組DT3C產品在抗體內化檢測中的生物活性?如果是的話,能否分享生物活性的驗證數據?
同樣作為ADC藥物抗體內化檢測的工具,與Mab-ZAP相比,DT3C的優(yōu)勢是什么?
我們已經訂購了1 mg的重組DT3C蛋白(CSB-EP360556CQR1),對測試結果非常滿意,我們希望批量訂購至少5 mg,是否有更多折扣?您是否有相同批號的現貨?如果沒有,交貨周期是多久?
剛剛收到你們關于介紹DT3C用于抗體內化檢測的郵件,想咨詢一下。我有一種類似于ADC的藥物,但連接體前面的所有部分都是合成的,可以用DT3C來檢測內化以及內化速率嗎?
DT3C是否只有BMK的殺傷效應?
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